Małgorzata Duda , Patrycja Maciak , Grzegorz Tylko , Kamil Wartalski , Monika Trzcińska , Marek Romek , Marcin Samiec
{"title":"研究报告：利用鸡胚皮细胞的潜力作为家禽科学和生物技术的新前沿——胚胎干细胞的建立及其向雪旺细胞样细胞系的分化。","authors":"Małgorzata Duda , Patrycja Maciak , Grzegorz Tylko , Kamil Wartalski , Monika Trzcińska , Marek Romek , Marcin Samiec","doi":"10.1016/j.psj.2024.104701","DOIUrl":null,"url":null,"abstract":"<div><div>The nervous system's regenerative potential has sparked interest in exploring novel approaches to generate Schwann cell-like cells (<strong>SC-LCs</strong>) from chicken blastoderm (<strong>B</strong>)-derived embryonic stem cells (<strong>B-ESCs</strong>). This study investigates the hypothesis that specific growth factors, when used during ex-ovo culture, can induce the differentiation of chicken B-ESCs into cells resembling Schwann cells (<strong>SCs</strong>). Blastodermal cells (<strong>BCs</strong>) were isolated from <em>in vivo</em>-fertilized eggs at stage X followed by 14-d proliferative culture (<strong>PRC</strong>) of B-ESCs and subsequent 14-d glial/neurolemmogenic differentiation culture (<strong>DFC</strong>). Western blot and immunofluorescence analyses were applied to identify ESC-related markers and SC-specific proteins. Ultimately, slender or triangular cells resembling early SCs, designated as SC-LCs, were generated. Pluripotency-related markers OCT4, SOX2, SSEA-4 and TRA-1-60 were detected in B-ESCs, while SC-specific markers such as GFAP and S-100β were identified in neurolemmogenically differentiated B-ESC derivatives (<strong>SC-LCs</strong>). The current study demonstrates, for the first time, the successful differentiation of chicken B-ESCs into SC-LCs through ex-ovo sequential culture. After PRC termination, B-ESCs exhibited pluripotent characteristics as shown by the presence of OCT4, SOX2, SSEA-4 and TRA-1-60 markers. Subsequent DFC led to the acquisition of SC-like morphology by B-ESCs, confirmed by the expression of SC-specific markers GFAP and S-100β in the resulting SC-LCs. These findings highlight the potential of B-ESCs as a valuable source for propagating SC-LCs, with implications for regenerative medicine and neural/glial tissue engineering applications. Further research exploring the functional attributes of B-ESC-derived SC-LCs is required to elucidate their therapeutic potential in nerve reconstruction/repair.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 2","pages":"Article 104701"},"PeriodicalIF":3.8000,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11729637/pdf/","citationCount":"0","resultStr":"{\"title\":\"Research note: Harnessing the potential of chicken blastodermal cells as a new frontier in poultry science and biotechnology – Establishment of embryonic stem cells and their differentiability towards Schwann cell-like cell lineages\",\"authors\":\"Małgorzata Duda , Patrycja Maciak , Grzegorz Tylko , Kamil Wartalski , Monika Trzcińska , Marek Romek , Marcin Samiec\",\"doi\":\"10.1016/j.psj.2024.104701\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>The nervous system's regenerative potential has sparked interest in exploring novel approaches to generate Schwann cell-like cells (<strong>SC-LCs</strong>) from chicken blastoderm (<strong>B</strong>)-derived embryonic stem cells (<strong>B-ESCs</strong>). This study investigates the hypothesis that specific growth factors, when used during ex-ovo culture, can induce the differentiation of chicken B-ESCs into cells resembling Schwann cells (<strong>SCs</strong>). Blastodermal cells (<strong>BCs</strong>) were isolated from <em>in vivo</em>-fertilized eggs at stage X followed by 14-d proliferative culture (<strong>PRC</strong>) of B-ESCs and subsequent 14-d glial/neurolemmogenic differentiation culture (<strong>DFC</strong>). Western blot and immunofluorescence analyses were applied to identify ESC-related markers and SC-specific proteins. Ultimately, slender or triangular cells resembling early SCs, designated as SC-LCs, were generated. Pluripotency-related markers OCT4, SOX2, SSEA-4 and TRA-1-60 were detected in B-ESCs, while SC-specific markers such as GFAP and S-100β were identified in neurolemmogenically differentiated B-ESC derivatives (<strong>SC-LCs</strong>). The current study demonstrates, for the first time, the successful differentiation of chicken B-ESCs into SC-LCs through ex-ovo sequential culture. After PRC termination, B-ESCs exhibited pluripotent characteristics as shown by the presence of OCT4, SOX2, SSEA-4 and TRA-1-60 markers. Subsequent DFC led to the acquisition of SC-like morphology by B-ESCs, confirmed by the expression of SC-specific markers GFAP and S-100β in the resulting SC-LCs. These findings highlight the potential of B-ESCs as a valuable source for propagating SC-LCs, with implications for regenerative medicine and neural/glial tissue engineering applications. Further research exploring the functional attributes of B-ESC-derived SC-LCs is required to elucidate their therapeutic potential in nerve reconstruction/repair.</div></div>\",\"PeriodicalId\":20459,\"journal\":{\"name\":\"Poultry Science\",\"volume\":\"104 2\",\"pages\":\"Article 104701\"},\"PeriodicalIF\":3.8000,\"publicationDate\":\"2025-02-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11729637/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Poultry Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0032579124012793\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURE, DAIRY & ANIMAL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Poultry Science","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0032579124012793","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
Research note: Harnessing the potential of chicken blastodermal cells as a new frontier in poultry science and biotechnology – Establishment of embryonic stem cells and their differentiability towards Schwann cell-like cell lineages
The nervous system's regenerative potential has sparked interest in exploring novel approaches to generate Schwann cell-like cells (SC-LCs) from chicken blastoderm (B)-derived embryonic stem cells (B-ESCs). This study investigates the hypothesis that specific growth factors, when used during ex-ovo culture, can induce the differentiation of chicken B-ESCs into cells resembling Schwann cells (SCs). Blastodermal cells (BCs) were isolated from in vivo-fertilized eggs at stage X followed by 14-d proliferative culture (PRC) of B-ESCs and subsequent 14-d glial/neurolemmogenic differentiation culture (DFC). Western blot and immunofluorescence analyses were applied to identify ESC-related markers and SC-specific proteins. Ultimately, slender or triangular cells resembling early SCs, designated as SC-LCs, were generated. Pluripotency-related markers OCT4, SOX2, SSEA-4 and TRA-1-60 were detected in B-ESCs, while SC-specific markers such as GFAP and S-100β were identified in neurolemmogenically differentiated B-ESC derivatives (SC-LCs). The current study demonstrates, for the first time, the successful differentiation of chicken B-ESCs into SC-LCs through ex-ovo sequential culture. After PRC termination, B-ESCs exhibited pluripotent characteristics as shown by the presence of OCT4, SOX2, SSEA-4 and TRA-1-60 markers. Subsequent DFC led to the acquisition of SC-like morphology by B-ESCs, confirmed by the expression of SC-specific markers GFAP and S-100β in the resulting SC-LCs. These findings highlight the potential of B-ESCs as a valuable source for propagating SC-LCs, with implications for regenerative medicine and neural/glial tissue engineering applications. Further research exploring the functional attributes of B-ESC-derived SC-LCs is required to elucidate their therapeutic potential in nerve reconstruction/repair.
期刊介绍：
First self-published in 1921, Poultry Science is an internationally renowned monthly journal, known as the authoritative source for a broad range of poultry information and high-caliber research. The journal plays a pivotal role in the dissemination of preeminent poultry-related knowledge across all disciplines. As of January 2020, Poultry Science will become an Open Access journal with no subscription charges, meaning authors who publish here can make their research immediately, permanently, and freely accessible worldwide while retaining copyright to their work. Papers submitted for publication after October 1, 2019 will be published as Open Access papers.
An international journal, Poultry Science publishes original papers, research notes, symposium papers, and reviews of basic science as applied to poultry. This authoritative source of poultry information is consistently ranked by ISI Impact Factor as one of the top 10 agriculture, dairy and animal science journals to deliver high-caliber research. Currently it is the highest-ranked (by Impact Factor and Eigenfactor) journal dedicated to publishing poultry research. Subject areas include breeding, genetics, education, production, management, environment, health, behavior, welfare, immunology, molecular biology, metabolism, nutrition, physiology, reproduction, processing, and products.
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