Andrei V. Chaplin, Alla P. Pikina, Victoria A. Shcherbakova, Sofia R. Sokolova, Irina V. Podoprigora, Milana S. Das, Filipp A. Koshkin, Galit A. Ilyina, Iuliia A. Vasiliadis, Boris A. Efimov
{"title":"从特应性皮炎患儿粪便中分离的隐状副芽孢杆菌","authors":"Andrei V. Chaplin, Alla P. Pikina, Victoria A. Shcherbakova, Sofia R. Sokolova, Irina V. Podoprigora, Milana S. Das, Filipp A. Koshkin, Galit A. Ilyina, Iuliia A. Vasiliadis, Boris A. Efimov","doi":"10.1007/s00203-024-04221-y","DOIUrl":null,"url":null,"abstract":"<div><p>An obligate anaerobic, Gram-negative rod-shaped bacterial strain designated AD58<sup>T</sup> was isolated from the feces of a 3-year-old boy with atopic dermatitis. The closest species is <i>Parabacteroides fecalis</i> with 96.98% 16S rRNA gene identity. The average nucleotide identity value between AD58<sup>T</sup> and <i>P. fecalis</i> AGMB00274<sup>T</sup> is 85.0–85.4%. The circular genome sequence is 3.77 Mbp long with 43.5 mol% G + C content. The strain AD58<sup>T</sup> grows at 32–42 °C, its pH range for growth is 6.0–7.5. No growth is observed in the presence of 1% or higher NaCl concentrations. The major fatty acids are anteiso-C15: 0, iso-C15: 0, and summed feature 11 (iso-C17: 0 3–OH and/or C18: 2 DMA), and the predominant respiratory quinone is MK-9. Conditioned media from AD58<sup>T</sup> increased expression of IL-8 but decreased expression of TLR-4 in HT29 cells. Based on the described properties, we propose AD58<sup>T</sup> as the type strain of <i>Parabacteroides absconsus</i> sp. nov. (= VKM B-3630<sup>T</sup> = JCM 35468<sup>T</sup>).</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 1","pages":""},"PeriodicalIF":2.3000,"publicationDate":"2025-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00203-024-04221-y.pdf","citationCount":"0","resultStr":"{\"title\":\"Parabacteroides absconsus sp. nov., isolated from feces of a child with atopic dermatitis\",\"authors\":\"Andrei V. Chaplin, Alla P. Pikina, Victoria A. Shcherbakova, Sofia R. Sokolova, Irina V. Podoprigora, Milana S. Das, Filipp A. Koshkin, Galit A. Ilyina, Iuliia A. Vasiliadis, Boris A. Efimov\",\"doi\":\"10.1007/s00203-024-04221-y\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>An obligate anaerobic, Gram-negative rod-shaped bacterial strain designated AD58<sup>T</sup> was isolated from the feces of a 3-year-old boy with atopic dermatitis. The closest species is <i>Parabacteroides fecalis</i> with 96.98% 16S rRNA gene identity. The average nucleotide identity value between AD58<sup>T</sup> and <i>P. fecalis</i> AGMB00274<sup>T</sup> is 85.0–85.4%. The circular genome sequence is 3.77 Mbp long with 43.5 mol% G + C content. The strain AD58<sup>T</sup> grows at 32–42 °C, its pH range for growth is 6.0–7.5. No growth is observed in the presence of 1% or higher NaCl concentrations. The major fatty acids are anteiso-C15: 0, iso-C15: 0, and summed feature 11 (iso-C17: 0 3–OH and/or C18: 2 DMA), and the predominant respiratory quinone is MK-9. Conditioned media from AD58<sup>T</sup> increased expression of IL-8 but decreased expression of TLR-4 in HT29 cells. Based on the described properties, we propose AD58<sup>T</sup> as the type strain of <i>Parabacteroides absconsus</i> sp. nov. (= VKM B-3630<sup>T</sup> = JCM 35468<sup>T</sup>).</p></div>\",\"PeriodicalId\":8279,\"journal\":{\"name\":\"Archives of Microbiology\",\"volume\":\"207 1\",\"pages\":\"\"},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2025-01-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://link.springer.com/content/pdf/10.1007/s00203-024-04221-y.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Archives of Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s00203-024-04221-y\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Archives of Microbiology","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1007/s00203-024-04221-y","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Parabacteroides absconsus sp. nov., isolated from feces of a child with atopic dermatitis
An obligate anaerobic, Gram-negative rod-shaped bacterial strain designated AD58T was isolated from the feces of a 3-year-old boy with atopic dermatitis. The closest species is Parabacteroides fecalis with 96.98% 16S rRNA gene identity. The average nucleotide identity value between AD58T and P. fecalis AGMB00274T is 85.0–85.4%. The circular genome sequence is 3.77 Mbp long with 43.5 mol% G + C content. The strain AD58T grows at 32–42 °C, its pH range for growth is 6.0–7.5. No growth is observed in the presence of 1% or higher NaCl concentrations. The major fatty acids are anteiso-C15: 0, iso-C15: 0, and summed feature 11 (iso-C17: 0 3–OH and/or C18: 2 DMA), and the predominant respiratory quinone is MK-9. Conditioned media from AD58T increased expression of IL-8 but decreased expression of TLR-4 in HT29 cells. Based on the described properties, we propose AD58T as the type strain of Parabacteroides absconsus sp. nov. (= VKM B-3630T = JCM 35468T).
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