细菌糖蛋白工程的进展:对当前技术、新出现的挑战和未来方向的评述。

IF 12.1 1区 工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biotechnology advances Pub Date : 2025-01-02 DOI:10.1016/j.biotechadv.2024.108514
Ziyu Li, Yujie Wang, Xiaojing Zhao, Qing Meng, Guozhen Ma, Lijie Xie, Xiaolong Jiang, Yutao Liu, Di Huang
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引用次数: 0

摘要

蛋白质糖基化是指在氨基酸侧链上添加碳水化合物链,赋予蛋白质基本特性,在合成生物学应用中具有巨大潜力。尽管它很重要,但天然糖基化途径存在一些局限性,强调需要新的工具来更好地理解糖基结构、识别、代谢和生物合成,并促进生物相关糖蛋白的产生。由于细菌糖基化系统的不断发现和研究,细菌糖工程领域受到了极大的关注。通过利用蛋白聚糖偶联技术,已经成功地设计了一系列具有临床和诊断用途的有价值的糖蛋白。本文从合成生物学和代谢工程的角度综述了近年来细菌蛋白糖基化的研究进展,重点介绍了新的糖蛋白治疗药物和疫苗的开发。我们概述了使用原核糖基化平台生产高价值定制糖蛋白的概况,特别强调了四个关键要素:(i)糖基转移酶,(ii)载体蛋白,(iii)糖基供体和(iv)宿主细菌。这些元素的优化可以精确控制糖基化模式,从而提高最终产物的潜力。最后,我们讨论了利用合成生物学技术开发微生物糖工厂和无细胞系统以高效生产糖蛋白的挑战和未来前景。
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Advances in bacterial glycoprotein engineering: A critical review of current technologies, emerging challenges, and future directions.

Protein glycosylation, which involves the addition of carbohydrate chains to amino acid side chains, imparts essential properties to proteins, offering immense potential in synthetic biology applications. Despite its importance, natural glycosylation pathways present several limitations, highlighting the need for new tools to better understand glycan structures, recognition, metabolism, and biosynthesis, and to facilitate the production of biologically relevant glycoproteins. The field of bacterial glycoengineering has gained significant attention due to the ongoing discovery and study of bacterial glycosylation systems. By utilizing protein glycan coupling technology, a wide range of valuable glycoproteins for clinical and diagnostic purposes have been successfully engineered. This review outlines the recent advances in bacterial protein glycosylation from the perspective of synthetic biology and metabolic engineering, focusing on the development of new glycoprotein therapeutics and vaccines. We provide an overview of the production of high-value, customized glycoproteins using prokaryotic glycosylation platforms, with particular emphasis on four key elements: (i) glycosyltransferases, (ii) carrier proteins, (iii) glycosyl donors, and (iv) host bacteria. Optimization of these elements enables precise control over glycosylation patterns, thus enhancing the potential of the resulting products. Finally, we discuss the challenges and future prospects of leveraging synthetic biology technologies to develop microbial glyco-factories and cell-free systems for efficient glycoprotein production.

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来源期刊
Biotechnology advances
Biotechnology advances 工程技术-生物工程与应用微生物
CiteScore
25.50
自引率
2.50%
发文量
167
审稿时长
37 days
期刊介绍: Biotechnology Advances is a comprehensive review journal that covers all aspects of the multidisciplinary field of biotechnology. The journal focuses on biotechnology principles and their applications in various industries, agriculture, medicine, environmental concerns, and regulatory issues. It publishes authoritative articles that highlight current developments and future trends in the field of biotechnology. The journal invites submissions of manuscripts that are relevant and appropriate. It targets a wide audience, including scientists, engineers, students, instructors, researchers, practitioners, managers, governments, and other stakeholders in the field. Additionally, special issues are published based on selected presentations from recent relevant conferences in collaboration with the organizations hosting those conferences.
期刊最新文献
Electrode functional microorganisms in bioelectrochemical systems and its regulation: A review. Advancements of astaxanthin production in Haematococcus pluvialis: Update insight and way forward. Recent advances in synthetic biology toolkits and metabolic engineering of Ralstonia eutropha H16 for production of value-added chemicals. Advancement in synthetic gene circuits engineering: An alternative strategy for microRNA imaging and disease theranostics. Corrigendum to "Recent advances in enzyme-enhanced immunosensors" [Biotechnology Advances 53 (2021) 107867].
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