{"title":"长链非编码RNA PDIA3P与FAK/TGF-β通路在口腔黏膜下纤维化大鼠中的表达及相关性","authors":"Fan di Zhu, Yu Xuan Li, Ru Qian Guo, Yun Xu","doi":"10.1016/j.jormas.2025.102214","DOIUrl":null,"url":null,"abstract":"<p><p>Oral Submucous Fibrosis constitutes a severe condition affecting the lining of the mouth, which carries a risk of developing into oral squamous cell carcinoma, and seriously threaten individuals' lives. Currently, no specific treatment has been utilized to prevent OSF. The detailed mechanism of OSF is still elusive, and this study aims to clarify the mechanism of OSF.</p><p><strong>Methods: </strong>This study was conducted to elucidate the molecular mechanisms of the long noncoding RNA PDIA3P (lncRNA PDIA3P) and FAK/TGF-β pathway in OSF. The OSF mice model was established by injecting with injected with 1000 mg/L arecoline solution, then the blood samples and oral submucosal tissues were collected from all groups of rats for further testing. HE staining, immunofluorescence, Western blot, and qRT-PCR were then used to elucidate the molecular mechanisms of the molecular mechanisms of the lncRNA PDIA3P and FAK/TGF-β pathway in OSF.</p><p><strong>Results: </strong>The results showed that upregulation of the FAK/TGF-β signaling pathway can accelerate OSF process, while the downregulation of the FAK/TGF-β signaling pathway can prevent the OSF process. Similarly, enhanced activity of the lncRNA PDIA3P is implicated with OSF process, while reducing its level can served as a preventative method against the OSF development.</p><p><strong>Conclusion: </strong>Upregulating the lncRNA PDIA3P and FAK/TGF-β pathway can accelerate the process of OSF.</p>","PeriodicalId":56038,"journal":{"name":"Journal of Stomatology Oral and Maxillofacial Surgery","volume":" ","pages":"102214"},"PeriodicalIF":2.2000,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Expression and correlation of the long noncoding RNA PDIA3P and FAK/TGF-β pathway in oral submucous fibrosis rats.\",\"authors\":\"Fan di Zhu, Yu Xuan Li, Ru Qian Guo, Yun Xu\",\"doi\":\"10.1016/j.jormas.2025.102214\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Oral Submucous Fibrosis constitutes a severe condition affecting the lining of the mouth, which carries a risk of developing into oral squamous cell carcinoma, and seriously threaten individuals' lives. Currently, no specific treatment has been utilized to prevent OSF. The detailed mechanism of OSF is still elusive, and this study aims to clarify the mechanism of OSF.</p><p><strong>Methods: </strong>This study was conducted to elucidate the molecular mechanisms of the long noncoding RNA PDIA3P (lncRNA PDIA3P) and FAK/TGF-β pathway in OSF. The OSF mice model was established by injecting with injected with 1000 mg/L arecoline solution, then the blood samples and oral submucosal tissues were collected from all groups of rats for further testing. HE staining, immunofluorescence, Western blot, and qRT-PCR were then used to elucidate the molecular mechanisms of the molecular mechanisms of the lncRNA PDIA3P and FAK/TGF-β pathway in OSF.</p><p><strong>Results: </strong>The results showed that upregulation of the FAK/TGF-β signaling pathway can accelerate OSF process, while the downregulation of the FAK/TGF-β signaling pathway can prevent the OSF process. Similarly, enhanced activity of the lncRNA PDIA3P is implicated with OSF process, while reducing its level can served as a preventative method against the OSF development.</p><p><strong>Conclusion: </strong>Upregulating the lncRNA PDIA3P and FAK/TGF-β pathway can accelerate the process of OSF.</p>\",\"PeriodicalId\":56038,\"journal\":{\"name\":\"Journal of Stomatology Oral and Maxillofacial Surgery\",\"volume\":\" \",\"pages\":\"102214\"},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2025-01-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Stomatology Oral and Maxillofacial Surgery\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1016/j.jormas.2025.102214\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"Dentistry\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Stomatology Oral and Maxillofacial Surgery","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1016/j.jormas.2025.102214","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Dentistry","Score":null,"Total":0}
Expression and correlation of the long noncoding RNA PDIA3P and FAK/TGF-β pathway in oral submucous fibrosis rats.
Oral Submucous Fibrosis constitutes a severe condition affecting the lining of the mouth, which carries a risk of developing into oral squamous cell carcinoma, and seriously threaten individuals' lives. Currently, no specific treatment has been utilized to prevent OSF. The detailed mechanism of OSF is still elusive, and this study aims to clarify the mechanism of OSF.
Methods: This study was conducted to elucidate the molecular mechanisms of the long noncoding RNA PDIA3P (lncRNA PDIA3P) and FAK/TGF-β pathway in OSF. The OSF mice model was established by injecting with injected with 1000 mg/L arecoline solution, then the blood samples and oral submucosal tissues were collected from all groups of rats for further testing. HE staining, immunofluorescence, Western blot, and qRT-PCR were then used to elucidate the molecular mechanisms of the molecular mechanisms of the lncRNA PDIA3P and FAK/TGF-β pathway in OSF.
Results: The results showed that upregulation of the FAK/TGF-β signaling pathway can accelerate OSF process, while the downregulation of the FAK/TGF-β signaling pathway can prevent the OSF process. Similarly, enhanced activity of the lncRNA PDIA3P is implicated with OSF process, while reducing its level can served as a preventative method against the OSF development.
Conclusion: Upregulating the lncRNA PDIA3P and FAK/TGF-β pathway can accelerate the process of OSF.
期刊介绍：
J Stomatol Oral Maxillofac Surg publishes research papers and techniques - (guest) editorials, original articles, reviews, technical notes, case reports, images, letters to the editor, guidelines - dedicated to enhancing surgical expertise in all fields relevant to oral and maxillofacial surgery: from plastic and reconstructive surgery of the face, oral surgery and medicine, … to dentofacial and maxillofacial orthopedics.
Original articles include clinical or laboratory investigations and clinical or equipment reports. Reviews include narrative reviews, systematic reviews and meta-analyses.
All manuscripts submitted to the journal are subjected to peer review by international experts, and must:
Be written in excellent English, clear and easy to understand, precise and concise;
Bring new, interesting, valid information - and improve clinical care or guide future research;
Be solely the work of the author(s) stated;
Not have been previously published elsewhere and not be under consideration by another journal;
Be in accordance with the journal''s Guide for Authors'' instructions: manuscripts that fail to comply with these rules may be returned to the authors without being reviewed.
Under no circumstances does the journal guarantee publication before the editorial board makes its final decision.
The journal is indexed in the main international databases and is accessible worldwide through the ScienceDirect and ClinicalKey Platforms.
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