Ballue S.T. Dadjo , Armelle T. Mbaveng , Michael F. Kengne , Ornella D. Tsobeng , Giresse D.T. Fonjou , Victor Kuete
{"title":"喀麦隆沿海地区杜阿拉laquininie医院慢性肾病患者ESBL耐药基因的流行和多重耐药铜绿假单胞菌的粪便携带","authors":"Ballue S.T. Dadjo , Armelle T. Mbaveng , Michael F. Kengne , Ornella D. Tsobeng , Giresse D.T. Fonjou , Victor Kuete","doi":"10.1016/j.jiph.2024.102651","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><div><em>Pseudomonas aeruginosa</em> is an opportunistic Gram-negative bacterium capable of causing severe infections in immunocompromised patients such as those suffering from chronic kidney disease (CKD). This study aimed to determine the resistance profile of <em>Pseudomonas aeruginosa</em>, and the prevalence of extended-spectrum β-lactamase (ESBL) resistance genes in patients with chronic kidney disease.</div></div><div><h3>Methods</h3><div>The prevalence of <em>Pseudomonas aeruginosa</em> was investigated in 458 patients, including 197 CKD patients and 261 patients suffering from gastrointestinal infections. The study was conducted at Laquintinie Hospital in Douala from January 2022 to December 2023. Stool samples were used to isolate <em>Pseudomonas aeruginosa</em> on Cetrimide agar. Antibiotic susceptibility testing was carried out using the Kirby-Bauer diffusion method. extended-spectrum β-lactamase (ESBL) resistance genes were detected by the polymerase chain reaction (PCR) method.</div></div><div><h3>Results</h3><div>The prevalence of fecal carriage of <em>Pseudomonas aeruginosa</em> was 9.17 % (n = 42/458), including twenty-nine (69.05 %) in patients with chronic kidney disease and thirteen (30.95 %) in patients without chronic kidney disease. The <em>Pseudomonas aeruginosa</em> isolates had a high rate of resistance to ceftazidime (72.41 %) in patients with CKD compared to patients without CKD (69.23 %). All isolates had a high resistance to ticarcillin (93.10 % and 92.31 %). The prevalence of multidrug-resistant (MDR) <em>Pseudomonas aeruginosa</em> isolates was 73.81 %. The MDR <em>Pseudomonas aeruginosa</em> were higher (70.97 % <em>vs.</em> 29.03 %) in patients suffering from CKD compared to patients without CKD. Up to 85.71 % of the <em>Pseudomonas aeruginosa</em> isolates harbored at least one ESBL gene. The <em>bla</em><sub>TEM</sub> type gene (66.67 %) was the most frequently detected gene, followed by <em>bla</em><sub>CTX-M</sub> (61.90 %) and <em>bla</em><sub>SHV</sub> (47.62 %). ESBL resistance genes were more common in CKD patients (72.22 %) compared to patients without CKD (27.78 %).</div></div><div><h3>Conclusion</h3><div>These results demonstrate that antibiotics belonging to the carbapenem and aminoglycoside classes could be used for <em>Pseudomonas aeruginosa</em> infection. This highlights the importance of regular surveillance of multidrug resistance and extended-spectrum β-lactamase production for <em>Pseudomonas aeruginosa</em> infections in patients with chronic kidney disease.</div></div>","PeriodicalId":16087,"journal":{"name":"Journal of Infection and Public Health","volume":"18 2","pages":"Article 102651"},"PeriodicalIF":4.7000,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Prevalence of ESBL resistance genes and fecal carriage of multidrug-resistant Pseudomonas aeruginosa isolates from patients with chronic kidney disease at the Laquintinie Hospital in Douala, Littoral Region, Cameroon\",\"authors\":\"Ballue S.T. Dadjo , Armelle T. Mbaveng , Michael F. Kengne , Ornella D. Tsobeng , Giresse D.T. Fonjou , Victor Kuete\",\"doi\":\"10.1016/j.jiph.2024.102651\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><div><em>Pseudomonas aeruginosa</em> is an opportunistic Gram-negative bacterium capable of causing severe infections in immunocompromised patients such as those suffering from chronic kidney disease (CKD). This study aimed to determine the resistance profile of <em>Pseudomonas aeruginosa</em>, and the prevalence of extended-spectrum β-lactamase (ESBL) resistance genes in patients with chronic kidney disease.</div></div><div><h3>Methods</h3><div>The prevalence of <em>Pseudomonas aeruginosa</em> was investigated in 458 patients, including 197 CKD patients and 261 patients suffering from gastrointestinal infections. The study was conducted at Laquintinie Hospital in Douala from January 2022 to December 2023. Stool samples were used to isolate <em>Pseudomonas aeruginosa</em> on Cetrimide agar. Antibiotic susceptibility testing was carried out using the Kirby-Bauer diffusion method. extended-spectrum β-lactamase (ESBL) resistance genes were detected by the polymerase chain reaction (PCR) method.</div></div><div><h3>Results</h3><div>The prevalence of fecal carriage of <em>Pseudomonas aeruginosa</em> was 9.17 % (n = 42/458), including twenty-nine (69.05 %) in patients with chronic kidney disease and thirteen (30.95 %) in patients without chronic kidney disease. The <em>Pseudomonas aeruginosa</em> isolates had a high rate of resistance to ceftazidime (72.41 %) in patients with CKD compared to patients without CKD (69.23 %). All isolates had a high resistance to ticarcillin (93.10 % and 92.31 %). The prevalence of multidrug-resistant (MDR) <em>Pseudomonas aeruginosa</em> isolates was 73.81 %. The MDR <em>Pseudomonas aeruginosa</em> were higher (70.97 % <em>vs.</em> 29.03 %) in patients suffering from CKD compared to patients without CKD. Up to 85.71 % of the <em>Pseudomonas aeruginosa</em> isolates harbored at least one ESBL gene. The <em>bla</em><sub>TEM</sub> type gene (66.67 %) was the most frequently detected gene, followed by <em>bla</em><sub>CTX-M</sub> (61.90 %) and <em>bla</em><sub>SHV</sub> (47.62 %). ESBL resistance genes were more common in CKD patients (72.22 %) compared to patients without CKD (27.78 %).</div></div><div><h3>Conclusion</h3><div>These results demonstrate that antibiotics belonging to the carbapenem and aminoglycoside classes could be used for <em>Pseudomonas aeruginosa</em> infection. This highlights the importance of regular surveillance of multidrug resistance and extended-spectrum β-lactamase production for <em>Pseudomonas aeruginosa</em> infections in patients with chronic kidney disease.</div></div>\",\"PeriodicalId\":16087,\"journal\":{\"name\":\"Journal of Infection and Public Health\",\"volume\":\"18 2\",\"pages\":\"Article 102651\"},\"PeriodicalIF\":4.7000,\"publicationDate\":\"2025-02-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Infection and Public Health\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S187603412400385X\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Infection and Public Health","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S187603412400385X","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
Prevalence of ESBL resistance genes and fecal carriage of multidrug-resistant Pseudomonas aeruginosa isolates from patients with chronic kidney disease at the Laquintinie Hospital in Douala, Littoral Region, Cameroon
Background
Pseudomonas aeruginosa is an opportunistic Gram-negative bacterium capable of causing severe infections in immunocompromised patients such as those suffering from chronic kidney disease (CKD). This study aimed to determine the resistance profile of Pseudomonas aeruginosa, and the prevalence of extended-spectrum β-lactamase (ESBL) resistance genes in patients with chronic kidney disease.
Methods
The prevalence of Pseudomonas aeruginosa was investigated in 458 patients, including 197 CKD patients and 261 patients suffering from gastrointestinal infections. The study was conducted at Laquintinie Hospital in Douala from January 2022 to December 2023. Stool samples were used to isolate Pseudomonas aeruginosa on Cetrimide agar. Antibiotic susceptibility testing was carried out using the Kirby-Bauer diffusion method. extended-spectrum β-lactamase (ESBL) resistance genes were detected by the polymerase chain reaction (PCR) method.
Results
The prevalence of fecal carriage of Pseudomonas aeruginosa was 9.17 % (n = 42/458), including twenty-nine (69.05 %) in patients with chronic kidney disease and thirteen (30.95 %) in patients without chronic kidney disease. The Pseudomonas aeruginosa isolates had a high rate of resistance to ceftazidime (72.41 %) in patients with CKD compared to patients without CKD (69.23 %). All isolates had a high resistance to ticarcillin (93.10 % and 92.31 %). The prevalence of multidrug-resistant (MDR) Pseudomonas aeruginosa isolates was 73.81 %. The MDR Pseudomonas aeruginosa were higher (70.97 % vs. 29.03 %) in patients suffering from CKD compared to patients without CKD. Up to 85.71 % of the Pseudomonas aeruginosa isolates harbored at least one ESBL gene. The blaTEM type gene (66.67 %) was the most frequently detected gene, followed by blaCTX-M (61.90 %) and blaSHV (47.62 %). ESBL resistance genes were more common in CKD patients (72.22 %) compared to patients without CKD (27.78 %).
Conclusion
These results demonstrate that antibiotics belonging to the carbapenem and aminoglycoside classes could be used for Pseudomonas aeruginosa infection. This highlights the importance of regular surveillance of multidrug resistance and extended-spectrum β-lactamase production for Pseudomonas aeruginosa infections in patients with chronic kidney disease.
期刊介绍：
The Journal of Infection and Public Health, first official journal of the Saudi Arabian Ministry of National Guard Health Affairs, King Saud Bin Abdulaziz University for Health Sciences and the Saudi Association for Public Health, aims to be the foremost scientific, peer-reviewed journal encompassing infection prevention and control, microbiology, infectious diseases, public health and the application of healthcare epidemiology to the evaluation of health outcomes. The point of view of the journal is that infection and public health are closely intertwined and that advances in one area will have positive consequences on the other.
The journal will be useful to all health professionals who are partners in the management of patients with communicable diseases, keeping them up to date. The journal is proud to have an international and diverse editorial board that will assist and facilitate the publication of articles that reflect a global view on infection control and public health, as well as emphasizing our focus on supporting the needs of public health practitioners.
It is our aim to improve healthcare by reducing risk of infection and related adverse outcomes by critical review, selection, and dissemination of new and relevant information in the field of infection control, public health and infectious diseases in all healthcare settings and the community.
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