Adriane Toledo da Silva , Debora Castro de Souza , Lisseth Bibiana Puentes Figueroa , Amanda do Carmo Alves , Tiago Moreira Facury , Eustáquio Souza Dias , Fábio Ribeiro Braga , Filippe Elias de Freitas Soares
{"title":"侧耳侧耳在共培养过程中产生的蛋白酶的蛋白水解谱和杀线虫潜能。","authors":"Adriane Toledo da Silva , Debora Castro de Souza , Lisseth Bibiana Puentes Figueroa , Amanda do Carmo Alves , Tiago Moreira Facury , Eustáquio Souza Dias , Fábio Ribeiro Braga , Filippe Elias de Freitas Soares","doi":"10.1016/j.rvsc.2025.105528","DOIUrl":null,"url":null,"abstract":"<div><div>The aim of this study was to evaluate the proteolytic profile of the cell-free crude extract (CFCE) of <em>Pleurotus djamor</em> and its nematicidal action on <em>Haemonchus</em> spp. and <em>Trichostrongylus</em> spp. larvae in coprocultures. Solid-state fermentation was used to produce proteases by <em>P. djamor</em>. The proteolytic and specific activity of CFCE was measured, and its proteolytic profile was revealed using a zymogram. The nematicidal assay of CFCE in coprocultures was evaluated using two groups: the control group (G1) containing 1 mL of CFCE with denatured enzymes +10 g of positive feces, and the treated group (G2) containing 1 mL of active CFCE +10 g of positive feces. For each group, 10 repetitions were carried out. A proteolytic activity of 7.5 ± 0.9 U mL<sup>−1</sup> and a specific activity of 30 ± U mg<sup>−1</sup> were measured. With regard to the nematicidal assay, there was a significant 35 % reduction in the number of larvae recovered from G2 compared to G1. The zymogram showed the presence of at least two proteases in the active CFCE of <em>P. djamor</em> with molecular weights between 75 and 100 kDa. The results suggest that the CFCE of <em>P. djamor</em> has nematicidal potential even in the feces environment.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"184 ","pages":"Article 105528"},"PeriodicalIF":2.2000,"publicationDate":"2025-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Proteolytic profile and nematicidal potential of proteases produced by Pleurotus djamor in coprocultures\",\"authors\":\"Adriane Toledo da Silva , Debora Castro de Souza , Lisseth Bibiana Puentes Figueroa , Amanda do Carmo Alves , Tiago Moreira Facury , Eustáquio Souza Dias , Fábio Ribeiro Braga , Filippe Elias de Freitas Soares\",\"doi\":\"10.1016/j.rvsc.2025.105528\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>The aim of this study was to evaluate the proteolytic profile of the cell-free crude extract (CFCE) of <em>Pleurotus djamor</em> and its nematicidal action on <em>Haemonchus</em> spp. and <em>Trichostrongylus</em> spp. larvae in coprocultures. Solid-state fermentation was used to produce proteases by <em>P. djamor</em>. The proteolytic and specific activity of CFCE was measured, and its proteolytic profile was revealed using a zymogram. The nematicidal assay of CFCE in coprocultures was evaluated using two groups: the control group (G1) containing 1 mL of CFCE with denatured enzymes +10 g of positive feces, and the treated group (G2) containing 1 mL of active CFCE +10 g of positive feces. For each group, 10 repetitions were carried out. A proteolytic activity of 7.5 ± 0.9 U mL<sup>−1</sup> and a specific activity of 30 ± U mg<sup>−1</sup> were measured. With regard to the nematicidal assay, there was a significant 35 % reduction in the number of larvae recovered from G2 compared to G1. The zymogram showed the presence of at least two proteases in the active CFCE of <em>P. djamor</em> with molecular weights between 75 and 100 kDa. The results suggest that the CFCE of <em>P. djamor</em> has nematicidal potential even in the feces environment.</div></div>\",\"PeriodicalId\":21083,\"journal\":{\"name\":\"Research in veterinary science\",\"volume\":\"184 \",\"pages\":\"Article 105528\"},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2025-01-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Research in veterinary science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0034528825000025\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Research in veterinary science","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0034528825000025","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
Proteolytic profile and nematicidal potential of proteases produced by Pleurotus djamor in coprocultures
The aim of this study was to evaluate the proteolytic profile of the cell-free crude extract (CFCE) of Pleurotus djamor and its nematicidal action on Haemonchus spp. and Trichostrongylus spp. larvae in coprocultures. Solid-state fermentation was used to produce proteases by P. djamor. The proteolytic and specific activity of CFCE was measured, and its proteolytic profile was revealed using a zymogram. The nematicidal assay of CFCE in coprocultures was evaluated using two groups: the control group (G1) containing 1 mL of CFCE with denatured enzymes +10 g of positive feces, and the treated group (G2) containing 1 mL of active CFCE +10 g of positive feces. For each group, 10 repetitions were carried out. A proteolytic activity of 7.5 ± 0.9 U mL−1 and a specific activity of 30 ± U mg−1 were measured. With regard to the nematicidal assay, there was a significant 35 % reduction in the number of larvae recovered from G2 compared to G1. The zymogram showed the presence of at least two proteases in the active CFCE of P. djamor with molecular weights between 75 and 100 kDa. The results suggest that the CFCE of P. djamor has nematicidal potential even in the feces environment.
期刊介绍：
Research in Veterinary Science is an International multi-disciplinary journal publishing original articles, reviews and short communications of a high scientific and ethical standard in all aspects of veterinary and biomedical research.
The primary aim of the journal is to inform veterinary and biomedical scientists of significant advances in veterinary and related research through prompt publication and dissemination. Secondly, the journal aims to provide a general multi-disciplinary forum for discussion and debate of news and issues concerning veterinary science. Thirdly, to promote the dissemination of knowledge to a broader range of professions, globally.
High quality papers on all species of animals are considered, particularly those considered to be of high scientific importance and originality, and with interdisciplinary interest. The journal encourages papers providing results that have clear implications for understanding disease pathogenesis and for the development of control measures or treatments, as well as those dealing with a comparative biomedical approach, which represents a substantial improvement to animal and human health.
Studies without a robust scientific hypothesis or that are preliminary, or of weak originality, as well as negative results, are not appropriate for the journal. Furthermore, observational approaches, case studies or field reports lacking an advancement in general knowledge do not fall within the scope of the journal.
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