Research in veterinary science最新文献

{"title":"Investigation of surfactant apoproteins and Brucella sp. antigens in the lungs of aborted bovine fetuses and neonatal calves delivered weak","authors":"Enver Beytut ,&nbsp;Mahmut Sözmen ,&nbsp;Emin Karakurt ,&nbsp;Hilmi Nuhoğlu","doi":"10.1016/j.rvsc.2024.105445","DOIUrl":"10.1016/j.rvsc.2024.105445","url":null,"abstract":"<div><div>The main objectives of this study were to investigate surfactant apoprotein expression (SP) and to detect <em>Brucella</em> sp. antigens in the lungs of aborted bovine fetuses and neonatal calves delivered weak. The Avidin-Biotin-Peroxidase Complex (ABC) and the indirect immunofluorescence (IF) techniques were applied, using antibodies to the lung surfactant apoproteins (SP-A, SP-B, SP-C) and <em>Brucella</em> sp. antigens. Hyperplasia of type II cells was also assessed by evaluating Thyroid Transcription Factor-1 (TTF-1), Proliferating Cell Nuclear Antigen (PCNA), and Cytokeratin Pan Type I/II (CK-P) markers. The study materials were the lungs of 46 aborted bovine fetuses and 20 neonatal calves delivered weak. <em>Brucella</em> sp.-positive fetal lungs displayed bronchopneumonia in 24 cases. The lungs of the weak-delivered neonates which were positive for <em>Brucella</em> sp. also showed pneumonia. Bacterial culture detected positivity in 11 of 46 fetuses and two neonates. IHC for <em>Brucella</em> sp. antigens found positivity in 22 of 46 fetuses and four neonates. Thus, our research revealed that the IHC technique using anti-<em>Brucella</em> sp. antibodies was useful for detecting <em>Brucella</em> sp. in autolytic and culture-negative fetuses. The study also found that surfactant synthesis begins close to the 7th month of gestation in bovine fetuses. Immunolabeling to SPs occurred in the cytoplasm of both type II and Clara cells, along with SP-C only in type II pneumocytes. The IF yielded dense labeling for <em>Brucella</em> sp. antigens, SP-B, and CK-P, respectively, in the phagocytic cells and epithelium of the airways. Also, pneumonia in newborn calves indicates an intrauterine infection by <em>Brucella</em> sp.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"181 ","pages":"Article 105445"},"PeriodicalIF":2.2,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142626697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Treatment modalities for claw horn lesions and their effects on locomotion scores, gait properties, lesion progression, and nociceptive threshold in dairy cows: A systematic review","authors":"Mohammed Babatunde Sadiq ,&nbsp;Siti Zubaidah Ramanoon ,&nbsp;Sharifah Salmah Syed-Hussain ,&nbsp;Rozaihan Mansor ,&nbsp;Wan Mastura Shaik Mossadeq ,&nbsp;Nurhusien Yimer Degu","doi":"10.1016/j.rvsc.2024.105448","DOIUrl":"10.1016/j.rvsc.2024.105448","url":null,"abstract":"<div><div>This systematic review presents scientific evidence on treatment modalities for claw horn lesions (CHLs) in dairy cows, and their efficacy in restoring normal gait, resolution of claw lesions, and improving nociceptive threshold. A literature search was performed in four databases leading to the synthesis of 10 articles for the final analysis. The types of treatment administered include single (STM), bimodal (BTM) and multimodal treatment modalities (MTM). Locomotion scores (LS) were the most reported outcome measure, followed by the resolution of claw lesion, time to lameness cure, nociceptive threshold and gait properties. Most pairwise comparisons for LS and nociceptive threshold depicted no significant difference between the various treatment groups. Meanwhile, a few pairwise comparisons in terms of resolution of claw lesion and time to lameness cure revealed that cows receiving MTM (claw trimming + hoof block + NSAID) or BTM (claw trimming + hoof block) recorded complete resolution of CHL and significantly less time to recover compared to those treated with an STM (only claw trimming). Despite the various modalities used in CHL treatment, their effects on LS, nociceptive threshold, and gait properties are still unclear. While evidence suggests that MTM and BTM facilitate rapid resolution of CHL and duration of lameness cure, only limited data are available and the association with specific lesions is poorly understood. Several sources of heterogeneity were identified between the studies, particularly duration of follow-up after treatment, LS systems, severity and duration of lameness, and types of treatment administered.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"181 ","pages":"Article 105448"},"PeriodicalIF":2.2,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142626711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Incidence of puerperal metritis and associated risk factors in dairy cows in Hawassa, Southern Ethiopia","authors":"Melese Alemu ,&nbsp;Kassaye Aragaw ,&nbsp;Desie Sheferaw ,&nbsp;Berhanu Sibhat ,&nbsp;Rahmeto Abebe ,&nbsp;Mesele Abera ,&nbsp;Sharon Egan ,&nbsp;Kassahun Asmare","doi":"10.1016/j.rvsc.2024.105447","DOIUrl":"10.1016/j.rvsc.2024.105447","url":null,"abstract":"<div><div>Metritis has considerable economic impact on dairy herd profitability due largely to reduced reproductive performance. Studies in Ethiopia only report prevalence, with no available data on the incidence of metritis. The present prospective cohort study aimed to estimate the incidence of puerperal metritis, identify risk factors, and isolate the causative bacteria in dairy cows in Hawassa. Daily follow-up of 120 dairy cows from parturition to 21 days postpartum was carried out from November 2019 to February 2021. Of these, 21 developed puerperal metritis with an incidence rate of 0.94 cases per 100 cow days at risk (95 % CI: 0.6–1.4). The cumulative incidence was found to be 17.5 % (95 % CI: 11.8–25.6). Over 90 % of the puerperal metritis occurred within the first 10 days of parturition. The mean day of occurrence of puerperal metritis was 7.7 days postpartum and the mean rectal temperature in metritic cows was 40.5 °C. Retained fetal membranes (RFM) (<em>p</em> &lt; 0.001) emerged as the only predisposing factor for metritis in the final Cox regression model. Cows with RFM faced a 14.9 times higher risk of metritis compared to cows without RFM. A total of 52 bacterial isolates, belonging to 4 genera, were recovered from the aerobic culture of 21 uterine swab samples. <em>E. coli</em> and <em>Staphylococcus</em> spp. were the most frequently isolated bacteria, with recovery from 21 (100 %) and 20 (95.2 %) of the metritic uteri, respectively. Results of this study suggested the importance of reducing incidence of RFM to reduce incidence of metritis in dairy cows.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"181 ","pages":"Article 105447"},"PeriodicalIF":2.2,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142626604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficiency of infrared pyrometer and infrared thermography for assessing body surface temperature in hair sheep","authors":"Wilder Hernando Ortiz Vega ,&nbsp;Robson Mateus Freitas Silveira ,&nbsp;Ana Carolina Barros de Freitas ,&nbsp;Celia Raquel Quirino","doi":"10.1016/j.rvsc.2024.105450","DOIUrl":"10.1016/j.rvsc.2024.105450","url":null,"abstract":"<div><div>The objectives of this study were: (i) to verify and compare the coat surface temperature (CST) of regions of interest (ROI) measured through infrared pyrometer (IRP) and thermography (IRT) under two environmental conditions, to evaluate (ii) reliability and (iii) sensitivity of the two infrared equipments to the environmental effect. Twenty hair ewes <em>(Ovis aries),</em> multiparous, non-lactating, non-pregnant, with body weight 59.38 ± 6.23 kg and body score condition ranging from 2.5 to 4.5 were evaluated. All CST, in both infrared methods, were higher (<em>P</em> &lt; 0.001) in the afternoon (2 to 3 p.m.), with the exception of eyes surface temperature (TSEY) from IRT, which was higher (<em>P</em> &lt; 0.001) in the morning (8 to 9 a.m.). Regarding the infrared methods at different times (twice a day, morning and afternoon), of the day, CST values for all ROIs were significantly higher (<em>P</em> &lt; 0.001) in thermography, regardless of the time shift, except for ear pinna surface temperature (TSE) and rectum surface temperature (TSR), which showed no significant difference (<em>P</em> &gt; 0.05) between the infrared methods during the morning shift. The thermal environment significantly influences (<em>P</em> &lt; 0.001) the surface temperature of various anatomical regions in both infrared methods. The correlation coefficients between IRT and IRP were moderate. The IRT is more sensitive to the thermal environment, as it presented higher canonical coefficients in the environmental variables. Among surface temperatures, eye surface temperature is the most influenced by variables in the thermal environment, especially THI. All three methods (digital thermometer-DT, IRT and IRP) showed &gt;80 % of the data variation in the first two components. Using the DT, we observed an association between vaginal temperature (VT) and RT with air temperature (AT) and temperature humidity index (THI), which was similar to the infrared thermography pattern, in which all ROIs were highly correlated with AT and THI. The opposite was observed in the pyrometer, in which an association between eye temperature and RH and lower factor loadings of the other ROIs with AT and THI in the first component was observed. In conclusion, while IRP and IRT showed similar performance in the morning, IRT proved to be more accurate in the afternoon, demonstrating a higher success rate in classifying the group of origin. Therefore, it is concluded that IRT is more reliable than IRP in detecting surface temperature changes under high temperatures.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"181 ","pages":"Article 105450"},"PeriodicalIF":2.2,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142604611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anethole improves mitochondrial activity and quality parameters in fresh and frozen-thawed ovine semen","authors":"Rachel F. Braga ,&nbsp;Lucas F.L. Correia ,&nbsp;Mariana P.P. Guimarães ,&nbsp;Nathalia O. Barbosa ,&nbsp;Thais G. de Oliveira ,&nbsp;Andreza A. da Silva ,&nbsp;Glaucia M. Bragança ,&nbsp;Felipe Z. Brandão ,&nbsp;Bruna R.C. Alves ,&nbsp;Joanna M.G. Souza-Fabjan","doi":"10.1016/j.rvsc.2024.105446","DOIUrl":"10.1016/j.rvsc.2024.105446","url":null,"abstract":"<div><div>Anethole, an antioxidant found in plants, appears to improve the survival of spermatozoa during semen cryopreservation. This study assessed the effects of commercial trans-anethole in ram semen cryopreservation. Thirty ejaculates from six rams were diluted in media containing anethole at the following concentrations: CONT (0 μM), AN10 (10 μM), AN50 (50 μM), and AN100 (100 μM). Semen was slow-frozen, preserved in liquid nitrogen, and thawed. Anethole at 10 μM or 50 μM did not compromise any studied sperm quality parameter but increased pre-freezing functionality of membrane and mitochondrial activity. At 10 μM, anethole reduced post-thawing spermatozoa lipoperoxidation. At 50 μM, anethole sustained higher mitochondrial activity after thawing, reduced minor defects in sperm, and increased the number of sperm binding to perivitelline membrane, while keeping lipoperoxidation levels as in control. Anethole at 100 μM promoted higher pre- and post-freezing mitochondrial activity and higher number of sperm binding to perivitelline membrane, in comparison to control. Additionally, some post-thawing kinematic parameters were enhanced by anethole at 100 μM. Of note, mitochondrial activity and lipoperoxidation were higher with anethole at 100 μM in comparison to 50 μM, not differing from control. At the hypoosmotic test, the highest concentration (100 μM) tested reduced sperm osmotic resistance. The results of this study indicate that using anethole in cryopreservation media promoted mostly positive effects on the fresh and post-thawed ram semen, and the advantages vary according to its concentration.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"181 ","pages":"Article 105446"},"PeriodicalIF":2.2,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142626602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A replacement approach to rodent model for the toxicity evaluation of gram-positive autogenous vaccine","authors":"Martina Angela Checco ,&nbsp;Riccardo Villa ,&nbsp;Andrea Cacciamali ,&nbsp;Silvia Dotti","doi":"10.1016/j.rvsc.2024.105443","DOIUrl":"10.1016/j.rvsc.2024.105443","url":null,"abstract":"<div><div>The Abnormal Toxicity Test (ATT) is an <em>in vivo</em> assay traditionally used in the autogenous vaccine release. That test, commonly applied to verify vaccine quality, is subject to ethical and practical limitations and should be removed as reference methods.</div><div>This approach is necessary to update the regulatory framework and to apply the 3Rs principle in the pharmaceutical field.</div><div>In this study, two <em>in vitro</em> cell viability assays were used to evaluate the potential toxicity of autogenous vaccines. Cells were treated with base-2 dilutions of autogenous vaccines, and viability was detected by MTT and ATP assays.</div><div>Differences and similarities between these tests were analysed. The ATP test showed good cell viability at 1:32 or 1:64 vaccine dilutions. The MTT assay gave discordant results and vaccines were not cytotoxic from the first dilutions. For this reason, a preference has been highlighted for the ATP method, a more sensitive test, capable of providing more accurate results.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"180 ","pages":"Article 105443"},"PeriodicalIF":2.2,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142561375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation on the in vitro effects of resveratrol on peripheral blood mononuclear cells harvested from healthy and atopic dogs","authors":"Domenico Santoro,&nbsp;Peter Nadeau,&nbsp;Linda Archer","doi":"10.1016/j.rvsc.2024.105441","DOIUrl":"10.1016/j.rvsc.2024.105441","url":null,"abstract":"<div><div>Canine atopic dermatitis (AD) is a common skin disease. Many therapeutic options are available to decrease inflammation and ameliorate pruritus. However, those treatments are associated with side effects, a long lag phase for efficacy, or high expense. Natural plant extracts have been identified as possible, safer alternatives to traditional anti-inflammatory and antipruritic drugs. Resveratrol has been revisited as a new, possible alternative for its numerous beneficial properties. The purpose of this study was to evaluate the in vitro effects of resveratrol on peripheral blood mononuclear cells (PBMC) harvested from healthy and atopic privately-owned dogs.</div><div>The PBMC harvested from nine healthy and 11 atopic dogs were isolated and exposed to four concentrations (1.5-9 μg/mL) of resveratrol both with or without phytohemagglutinin stimulation. After 24 h cytotoxicity, host defense peptides (HDPs), as well as oxidative stress (catalase and superoxide dismutase), and pro-inflammatory cytokines were assessed. Cytotoxicity was not observed in either group under any experimental conditions. An increase in catalase was only seen in healthy PBMC after exposure to low concentrations of resveratrol (<em>p</em> ≤ 0.03). Resveratrol did not show any effect on canine HDPs. Compared to baseline, there was a significant reduction in monocyte chemotactic protein-1 and interleukin-6 after exposure to 9 μg/mL of resveratrol in unstimulated healthy (<em>p</em> = 0.029) and stimulated atopic (<em>p</em> = 0.0075) PBMC. In conclusion, these data confirm the overall lack of cytotoxicity of resveratrol on healthy and atopic PBMC at the tested concentrations. However, at the concentrations tested, there was only a minimal effect of resveratrol on the secretion of HDPs and pro-inflammatory cytokines.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"180 ","pages":"Article 105441"},"PeriodicalIF":2.2,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142553138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary prebiotic alleviates experimentally induced coccidiosis in broilers","authors":"Mahnaz Bayat,&nbsp;Hassan Darmani Kuhi,&nbsp;Mohammad Roostaei-Ali Mehr,&nbsp;Navid Ghavi Hossein-Zadeh","doi":"10.1016/j.rvsc.2024.105440","DOIUrl":"10.1016/j.rvsc.2024.105440","url":null,"abstract":"<div><div>An experiment was conducted to determine the effects of prebiotic on growth performance and coccidiosis prevention in challenged broilers with Eimeria. A total of 420 1-d-old male Ross 308 chicks were used in a completely randomized design with 7 treatments and 5 replicates with 12 birds in each replication. Dietary treatments were: 1) negative control (without prebiotic and without challenge), 2) positive control (challenged with sporulated oocysts of Eimeria (SOE) without prebiotic), 3) 0.2 % prebiotic in starter, 0.1 % in grower and 0.05 % in finisher challenged with SOE, 4) 0.2 % prebiotic in starter,0.1 % in grower and 0.05 % in finisher without challenge, 5) 0.2 % prebiotic in the whole rearing period challenged with SOE, 6) 0.2 % prebiotic in the whole rearing period without challenge and 7) and Salinomycin (0.05 % of diet). At 7 d of age, treatments were challenged with 20-fold dose of the EIMERIAVAX 4 m as a trivalent live attenuated coccidiosis vaccine. On d 28, intestinal coccidiosis lesions and dropping were scored in the scale of 0–3 and 0–4, respectively, and oocysts per gram feces (OPG) were measured. Prebiotic at either supplementation rate increased body weight gain and improved feed conversion ratio compared with PC group. Challenged broilers fed fixed level of prebiotic displayed lower OPG, dropping scores and coccidiosis lesions scores in upper and middle regions of intestine than PC group, with the effect being similar to unchallenged birds.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"180 ","pages":"Article 105440"},"PeriodicalIF":2.2,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142547122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiological surveillance of Neospora caninum in sheep (Ovis aries) and European mouflon (Ovis aries musimon) in southern Spain","authors":"Débora Jiménez-Martín ,&nbsp;Ana Huertas-López ,&nbsp;José Joaquín Cerón ,&nbsp;Carlos Martínez-Carrasco ,&nbsp;Silvia Martínez-Subiela ,&nbsp;Paloma Prieto ,&nbsp;Sonia Almería ,&nbsp;David Cano-Terriza ,&nbsp;Ignacio García-Bocanegra","doi":"10.1016/j.rvsc.2024.105444","DOIUrl":"10.1016/j.rvsc.2024.105444","url":null,"abstract":"<div><div><em>Neospora caninum</em> is a protozoan with a facultative heteroxenous life cycle, with canids as the definitive hosts and other mammals, mainly ruminants, acting as intermediate hosts. This parasite is recognized as one of the major abortifacient pathogens in cattle. Although reproductive disorders have also been reported in other domestic and wild ruminants, epidemiological data on <em>N. caninum</em> in ruminant species other than cattle is still limited. Here, we evaluate the seroprevalence and risk factors associated with <em>N. caninum</em> exposure in sheep (<em>Ovis aries</em>) and European mouflon (<em>Ovis aries musimon</em>) in southern Spain. Serum samples from 390 sheep and 387 free-ranging mouflons were tested for antibodies against <em>N. caninum</em> using in-house time-resolved fluorescence immunoassay based on NcGRA7 as a recombinant antigen. The individual seroprevalence was 26.2 % (95 %CI: 22.0–30.7) in sheep and 5.7 % (95 %CI: 3.8–8.5) in mouflons. At least one seropositive animal was detected in all the 26 sheep farms (100 %) and in eight of the 18 (44.4 %) hunting estates sampled. The presence of dogs on the farm (≥3) and the sheep breed (purebred) were potential risk factors associated with exposure to <em>N. caninum</em> in sheep. Our results indicate a high circulation of <em>N. caninum</em> in sheep farms in southern Spain. Control measures should be implemented to limit the exposure to this protozoan in sheep flocks. Although we have confirmed for the first time the presence of anti- <em>N. caninum</em> antibodies in European mouflon in Spain, and, despite the relatively low seroprevalence found, the impact of this parasite on mouflon populations should be further studied.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"180 ","pages":"Article 105444"},"PeriodicalIF":2.2,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142584164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of intra and postoperative analgesia efficacy of intraperitoneal levobupivacaine alone or in combination with dexmedetomidine in cats undergoing ovariohysterectomy; a randomized, prospective, blinded, clinical trial","authors":"Damla Tuğçe Okur ,&nbsp;Alper Yasin Çiplak ,&nbsp;Şifanur Aydin ,&nbsp;Sıtkıcan Okur ,&nbsp;Ayşe Gölgeli Bedir ,&nbsp;Ömer Tarık Orhun ,&nbsp;Şaab Elban ,&nbsp;Vefa Tohumcu","doi":"10.1016/j.rvsc.2024.105442","DOIUrl":"10.1016/j.rvsc.2024.105442","url":null,"abstract":"<div><div>This study aimed to compare the analgesic and cardiopulmonary effects of levobupivacaine with or without dexmedetomidine, in cats undergoing ovariohysterectomy. In this prospective, randomized, and blinded clinical trial, 24 healthy cats were recruited. Animals received either saline (S group; 2 mL NaCl), levobupivacaine alone (Lev; 0.35 mg/kg), or levobupivacaine with dexmedetomidine (LevDex group; 0.004 mg/kg), which was infiltrated intraoperatively into the subcutaneous tissue at various incision sites, including the right and left ovarian pedicles and the caudal aspect of the uterine body. Intraoperative analgesia was evaluated using a cumulative pain scale, while postoperative analgesia was assessed at various time points: before surgery (T_b), and at 0 (T₀), 1 (T_1h), 2 (T_2h), 4 (T_4h), 8 (T_8h), 12 (T_12h), and 24 (T_24h) hours after the procedure, using the UNESP-Botucatu multidimensional composite pain scale (MCPS). Significant decreases in heart rate, respiratory rate, and mean arterial pressure were observed in LevDex group as compared to S and Lev groups (<em>p</em> &lt; 0.05). The S group required significantly more rescue morphine doses than the LevDex group (<em>p</em> = 0.029), but the difference was not significant when compared to the Lev group (<em>p</em> = 0.123). At T_1h and T_2h, the S group had significantly higher MCPS scores than both the Lev and LevDex groups (<em>p</em> = 0.029). However, at T_8h, no significant difference was found between the S and LevDex groups (<em>p</em> = 0.741). While adding dexmedetomidine to levobupivacaine may slightly prolong postoperative analgesia, this combination does not provide significant additional benefit over levobupivacaine alone in terms of response to surgical stimulation.</div></div>","PeriodicalId":21083,"journal":{"name":"Research in veterinary science","volume":"180 ","pages":"Article 105442"},"PeriodicalIF":2.2,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142522849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}