Myceliophthora thermophila as promising fungal cell factories for industrial bioproduction: From rational design to industrial applications.

Myceliophthora thermophila stands out as a prominent fungal cell factory, garnering growing interest due to its distinctive traits advantageous. Currently, M. thermophila has been developed as an efficient cell factory, producing a variety of products from various raw materials. In this review, we firstly discuss the potential advantages of M. thermophila as a platform for metabolic engineering and industrial applications, with special emphasis on its physiological characteristics, the development of genetic modification techniques and tools, gene expression and regulation strategies. Then, the latest progress in industrial application of M. thermophila as microbial cell factory was systematically summarized, including biochemical synthesis platform, enzyme expression platform, antibody protein and vaccine production platform, bio-organic fertilizer production platform, and efficient enzyme element library. Finally, the current challenges of M. thermophila as a cell factory and its corresponding strategies are proposed, aiming to achieve green biomanufacturing of multiple products with higher efficiency.