Mersa Darbandsari, Majid Asgari, Mohammad R Abaei, Zahra Ghorbanzadeh, Maryam Derikvand, Patrick Okwarah, Navid Dinparast Djadid, Abbasali Raz
{"title":"白纹伊蚊中肠微生物群的分离和鉴定,以确定合适的准突变候选物。","authors":"Mersa Darbandsari, Majid Asgari, Mohammad R Abaei, Zahra Ghorbanzadeh, Maryam Derikvand, Patrick Okwarah, Navid Dinparast Djadid, Abbasali Raz","doi":"10.1093/eurpub/ckae110","DOIUrl":null,"url":null,"abstract":"<p><p>Aedes albopictus is a widely recognized carrier of various pathogens. Its resilient characteristics enable it to easily spread across diverse climates. The microbiota in the midgut of mosquitoes plays a crucial role in the interactions between the host and pathogens and can either enhance or reduce the ability of the insect to transmit diseases. Hence, determining the microorganisms present in the mosquito's digestive system could be a promising approach to developing an effective method of controlling them. Hence, the aim of this study was to investigate the microbial compositions in the midguts of Ae. albopictus mosquitoes collected from the fields of Sistan and Baluchestan Province. The midguts of 60 female mosquitoes were dissected, and their related bacteria were determined using the culture-dependent method. Different colonies were differentiated using the biochemical tests followed by 16S rRNA gene sequencing. The isolated bacteria were identified as belonging to the Asaia, Delftia, Serratia, Aeromonas, Paracoccus, and Planomicrobium genera based on biochemical and molecular analysis. The findings obtained in this study were largely consistent with earlier studies conducted on mosquitoes gathered from different regions throughout the world. Overall, the findings could enhance our understanding of the microbial diversity in Ae. albopictus and aid in the identification of a potent and widespread bacterium for the development of a paratransgenesis tool to combat Aedes-borne infectious diseases.</p>","PeriodicalId":12059,"journal":{"name":"European Journal of Public Health","volume":"35 Supplement_1","pages":"i60-i65"},"PeriodicalIF":3.7000,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725955/pdf/","citationCount":"0","resultStr":"{\"title\":\"Isolation and characterization of the midgut microbiota of Aedes albopictus to identify suitable candidates for paratransgenesis.\",\"authors\":\"Mersa Darbandsari, Majid Asgari, Mohammad R Abaei, Zahra Ghorbanzadeh, Maryam Derikvand, Patrick Okwarah, Navid Dinparast Djadid, Abbasali Raz\",\"doi\":\"10.1093/eurpub/ckae110\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Aedes albopictus is a widely recognized carrier of various pathogens. Its resilient characteristics enable it to easily spread across diverse climates. The microbiota in the midgut of mosquitoes plays a crucial role in the interactions between the host and pathogens and can either enhance or reduce the ability of the insect to transmit diseases. Hence, determining the microorganisms present in the mosquito's digestive system could be a promising approach to developing an effective method of controlling them. Hence, the aim of this study was to investigate the microbial compositions in the midguts of Ae. albopictus mosquitoes collected from the fields of Sistan and Baluchestan Province. The midguts of 60 female mosquitoes were dissected, and their related bacteria were determined using the culture-dependent method. Different colonies were differentiated using the biochemical tests followed by 16S rRNA gene sequencing. The isolated bacteria were identified as belonging to the Asaia, Delftia, Serratia, Aeromonas, Paracoccus, and Planomicrobium genera based on biochemical and molecular analysis. The findings obtained in this study were largely consistent with earlier studies conducted on mosquitoes gathered from different regions throughout the world. Overall, the findings could enhance our understanding of the microbial diversity in Ae. albopictus and aid in the identification of a potent and widespread bacterium for the development of a paratransgenesis tool to combat Aedes-borne infectious diseases.</p>\",\"PeriodicalId\":12059,\"journal\":{\"name\":\"European Journal of Public Health\",\"volume\":\"35 Supplement_1\",\"pages\":\"i60-i65\"},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2025-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725955/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"European Journal of Public Health\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1093/eurpub/ckae110\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"European Journal of Public Health","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1093/eurpub/ckae110","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH","Score":null,"Total":0}
Isolation and characterization of the midgut microbiota of Aedes albopictus to identify suitable candidates for paratransgenesis.
Aedes albopictus is a widely recognized carrier of various pathogens. Its resilient characteristics enable it to easily spread across diverse climates. The microbiota in the midgut of mosquitoes plays a crucial role in the interactions between the host and pathogens and can either enhance or reduce the ability of the insect to transmit diseases. Hence, determining the microorganisms present in the mosquito's digestive system could be a promising approach to developing an effective method of controlling them. Hence, the aim of this study was to investigate the microbial compositions in the midguts of Ae. albopictus mosquitoes collected from the fields of Sistan and Baluchestan Province. The midguts of 60 female mosquitoes were dissected, and their related bacteria were determined using the culture-dependent method. Different colonies were differentiated using the biochemical tests followed by 16S rRNA gene sequencing. The isolated bacteria were identified as belonging to the Asaia, Delftia, Serratia, Aeromonas, Paracoccus, and Planomicrobium genera based on biochemical and molecular analysis. The findings obtained in this study were largely consistent with earlier studies conducted on mosquitoes gathered from different regions throughout the world. Overall, the findings could enhance our understanding of the microbial diversity in Ae. albopictus and aid in the identification of a potent and widespread bacterium for the development of a paratransgenesis tool to combat Aedes-borne infectious diseases.
期刊介绍:
The European Journal of Public Health (EJPH) is a multidisciplinary journal aimed at attracting contributions from epidemiology, health services research, health economics, social sciences, management sciences, ethics and law, environmental health sciences, and other disciplines of relevance to public health. The journal provides a forum for discussion and debate of current international public health issues, with a focus on the European Region. Bi-monthly issues contain peer-reviewed original articles, editorials, commentaries, book reviews, news, letters to the editor, announcements of events, and various other features.