通过沉淀法脱盐寡核苷酸用于质谱分析。

IF 1.1 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-01-13 DOI:10.1080/15257770.2025.2452387

Raissa Sultana, Eline Darjinoff, Hamza Qureshi, Liqun Qiu, Jonathon Roepke, Hongbin Yan

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引用次数: 0

摘要

在质谱分析中，寡核苷酸中钠离子的污染经常引起问题。本研究考察了乙酸铵与醇联合脱盐寡核苷酸的效果。结果表明，在1 M或5 M乙酸铵存在下，乙醇或异丙醇沉淀法可以有效地脱盐4 M NaCl的寡核苷酸样品。通过原子发射光谱测定，钠离子的水平降低了2-3个数量级。采用直接输注电喷雾电离质谱法对脱盐样品进行了分析。

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Desalting of oligonucleotides through precipitation for mass spectrometric analysis.

Contamination of sodium ions in oligonucleotides often causes issues in mass spectrometric analysis. This study investigated the efficiency of the combination of ammonium acetate and alcohol in desalting oligonucleotides. It was found that oligonucleotide samples containing up to 4 M NaCl can be effectively desalted through precipitation with ethanol or isopropanol in the presence of 1 or 5 M ammonium acetate. The level of sodium ions was reduced by 2-3 orders of magnitude as determined by atomic emission spectroscopy. The desalted samples were successfully analyzed by direct-infusion electrospray ionization mass spectrometry.

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来源期刊

Nucleosides, Nucleotides & Nucleic Acids 生物-生化与分子生物学

CiteScore

2.60

自引率

7.70%

发文量

审稿时长

6 months

期刊介绍： Nucleosides, Nucleotides & Nucleic Acids publishes research articles, short notices, and concise, critical reviews of related topics that focus on the chemistry and biology of nucleosides, nucleotides, and nucleic acids. Complete with experimental details, this all-inclusive journal emphasizes the synthesis, biological activities, new and improved synthetic methods, and significant observations related to new compounds.