Yui Mizumoto-Teramura , Akira Leon Yoshikawa , Naoyuki Matsumoto , Yoko Murayama , Ken Akashi
{"title":"使用近红外波长的甲状旁腺和甲状腺组织的荧光显微镜定位自身荧光物质。","authors":"Yui Mizumoto-Teramura , Akira Leon Yoshikawa , Naoyuki Matsumoto , Yoko Murayama , Ken Akashi","doi":"10.1016/j.anl.2024.12.007","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><div>The parathyroid gland emits autofluorescence with a peak at 822 nm when excited using near-infrared light at 785 nm; this observation of autofluorescence using a near-infrared detection device is useful for identifying the parathyroid gland during surgery. We aimed to clarify the localization of autofluorescent substances in parathyroid and thyroid tissues by observing them under a fluorescence microscope through filters that selectively pass specific near-infrared wavelengths.</div></div><div><h3>Methods</h3><div>Four cases of parathyroid and three cases of thyroid were examined under a fluorescence microscope. The frozen, formalin-fixed paraffin-embedded, and unfixed, unstained sections of parathyroid were observed through filters that selectively pass specific near-infrared wavelengths. Images were acquired at excitation 775 ± 50 nm and absorption 845 ± 55 nm in five randomly selected fields of view, avoiding tumor and inflammatory areas. Autofluorescence was measured as the ratio of fluorescent area to tissue area using hybrid cell counting.</div></div><div><h3>Results</h3><div>Autofluorescence was observed in all sections. In the parathyroid tissue, the frozen sections showed significantly more autofluorescence than the formalin-fixed paraffin-embedded sections, and in the thyroid tissue, although no significant difference was observed, the frozen sections showed more autofluorescence than the formalin-fixed paraffin-embedded sections. In addition, the single unfixed, unstained section showed stronger autofluorescence than the frozen sections, although no significant difference was found. The areas of autofluorescence in the parathyroid and thyroid tissues were thought to be the Golgi area and lipofuscin, respectively.</div></div><div><h3>Conclusion</h3><div>Fluorescence microscopy of parathyroid and thyroid tissues revealed the localization of autofluorescent substances in each tissue.</div></div>","PeriodicalId":55627,"journal":{"name":"Auris Nasus Larynx","volume":"52 1","pages":"Pages 71-75"},"PeriodicalIF":1.6000,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Fluorescence microscopy of parathyroid and thyroid tissues for localization of autofluorescent substances using near-infrared wavelengths\",\"authors\":\"Yui Mizumoto-Teramura , Akira Leon Yoshikawa , Naoyuki Matsumoto , Yoko Murayama , Ken Akashi\",\"doi\":\"10.1016/j.anl.2024.12.007\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objective</h3><div>The parathyroid gland emits autofluorescence with a peak at 822 nm when excited using near-infrared light at 785 nm; this observation of autofluorescence using a near-infrared detection device is useful for identifying the parathyroid gland during surgery. We aimed to clarify the localization of autofluorescent substances in parathyroid and thyroid tissues by observing them under a fluorescence microscope through filters that selectively pass specific near-infrared wavelengths.</div></div><div><h3>Methods</h3><div>Four cases of parathyroid and three cases of thyroid were examined under a fluorescence microscope. The frozen, formalin-fixed paraffin-embedded, and unfixed, unstained sections of parathyroid were observed through filters that selectively pass specific near-infrared wavelengths. Images were acquired at excitation 775 ± 50 nm and absorption 845 ± 55 nm in five randomly selected fields of view, avoiding tumor and inflammatory areas. Autofluorescence was measured as the ratio of fluorescent area to tissue area using hybrid cell counting.</div></div><div><h3>Results</h3><div>Autofluorescence was observed in all sections. In the parathyroid tissue, the frozen sections showed significantly more autofluorescence than the formalin-fixed paraffin-embedded sections, and in the thyroid tissue, although no significant difference was observed, the frozen sections showed more autofluorescence than the formalin-fixed paraffin-embedded sections. In addition, the single unfixed, unstained section showed stronger autofluorescence than the frozen sections, although no significant difference was found. The areas of autofluorescence in the parathyroid and thyroid tissues were thought to be the Golgi area and lipofuscin, respectively.</div></div><div><h3>Conclusion</h3><div>Fluorescence microscopy of parathyroid and thyroid tissues revealed the localization of autofluorescent substances in each tissue.</div></div>\",\"PeriodicalId\":55627,\"journal\":{\"name\":\"Auris Nasus Larynx\",\"volume\":\"52 1\",\"pages\":\"Pages 71-75\"},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2025-02-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Auris Nasus Larynx\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0385814624001548\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"OTORHINOLARYNGOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Auris Nasus Larynx","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0385814624001548","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"OTORHINOLARYNGOLOGY","Score":null,"Total":0}
Fluorescence microscopy of parathyroid and thyroid tissues for localization of autofluorescent substances using near-infrared wavelengths
Objective
The parathyroid gland emits autofluorescence with a peak at 822 nm when excited using near-infrared light at 785 nm; this observation of autofluorescence using a near-infrared detection device is useful for identifying the parathyroid gland during surgery. We aimed to clarify the localization of autofluorescent substances in parathyroid and thyroid tissues by observing them under a fluorescence microscope through filters that selectively pass specific near-infrared wavelengths.
Methods
Four cases of parathyroid and three cases of thyroid were examined under a fluorescence microscope. The frozen, formalin-fixed paraffin-embedded, and unfixed, unstained sections of parathyroid were observed through filters that selectively pass specific near-infrared wavelengths. Images were acquired at excitation 775 ± 50 nm and absorption 845 ± 55 nm in five randomly selected fields of view, avoiding tumor and inflammatory areas. Autofluorescence was measured as the ratio of fluorescent area to tissue area using hybrid cell counting.
Results
Autofluorescence was observed in all sections. In the parathyroid tissue, the frozen sections showed significantly more autofluorescence than the formalin-fixed paraffin-embedded sections, and in the thyroid tissue, although no significant difference was observed, the frozen sections showed more autofluorescence than the formalin-fixed paraffin-embedded sections. In addition, the single unfixed, unstained section showed stronger autofluorescence than the frozen sections, although no significant difference was found. The areas of autofluorescence in the parathyroid and thyroid tissues were thought to be the Golgi area and lipofuscin, respectively.
Conclusion
Fluorescence microscopy of parathyroid and thyroid tissues revealed the localization of autofluorescent substances in each tissue.
期刊介绍：
The international journal Auris Nasus Larynx provides the opportunity for rapid, carefully reviewed publications concerning the fundamental and clinical aspects of otorhinolaryngology and related fields. This includes otology, neurotology, bronchoesophagology, laryngology, rhinology, allergology, head and neck medicine and oncologic surgery, maxillofacial and plastic surgery, audiology, speech science.
Original papers, short communications and original case reports can be submitted. Reviews on recent developments are invited regularly and Letters to the Editor commenting on papers or any aspect of Auris Nasus Larynx are welcomed.
Founded in 1973 and previously published by the Society for Promotion of International Otorhinolaryngology, the journal is now the official English-language journal of the Oto-Rhino-Laryngological Society of Japan, Inc. The aim of its new international Editorial Board is to make Auris Nasus Larynx an international forum for high quality research and clinical sciences.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
