牛乳桥素通过改变肠道菌群组成和胆汁酸代谢促进生长大鼠骨发育。

IF 5.1 1区 农林科学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Food & Function Pub Date : 2025-02-03 DOI:10.1039/d4fo05555a
Yipin Lyu, Jianqiang Hu, Xinyan Wang, Jie Zhang, Xue Li, Mengjun Cui, Dasong Liu, Xue Tang, Peng Zhou
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引用次数: 0

摘要

乳桥蛋白(LPN)是一种重要的乳蛋白,具有改善骨骼健康的潜力;然而,其具体效果尚未确定。本研究旨在探讨LPN对早期骨生长发育的影响。将3只周龄SD大鼠(n = 32)分为对照组、乳清蛋白浓度组、低剂量LPN组、高剂量LPN组,分别灌胃去离子水、65.8 mg kg-1 WPC、5 mg kg-1 LPN和45 mg kg-1 LPN,持续30 d。补充LPN-H增加了体长和股骨长度,改善了股骨体积和微结构,上调了股骨中成骨细胞活性相关mRNA (Bmp2、Smad8和Runx-2)的表达。LPN-H干预后,粪便和血清中二次胆汁酸-糖colcolthocholic acid (GLCA)含量显著升高,且与粪便中拟副杆菌丰度升高呈正相关。此外,在LPN- h组中,股骨胆汁酸受体FXR也被激活,提示LPN可能通过微生物-代谢-骨轴调节骨骼健康。本研究结果提示LPN在调节生长过程中骨代谢方面具有潜在的应用价值。
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Bovine lactopontin promotes bone development in growing rats by altering the composition of intestinal flora and bile acid metabolism.

Lactopontin (LPN) is an important milk protein with the potential to improve bone health; however, its specific effects have not been determined. This study aims to investigate the effects of LPN on early bone growth and development. 3 week-old SD rats (n = 32) were assigned to the control group, whey protein concentration (WPC) group, LPN-L (low-dose LPN) group, and LPN-H (high-dose LPN) group, with intragastric administration of deionized water, 65.8 mg kg-1 WPC, and 5 and 45 mg kg-1 LPN for 30 days, respectively. LPN-H supplementation increased body length and femur length, improved femoral bone volume and microarchitecture, and upregulated osteoblast activity-related mRNA (Bmp2, Smad8, and Runx-2) expression in the femur. The content of secondary bile acid glycolithocholic acid (GLCA) in stool and serum was significantly increased after LPN-H intervention and positively correlated with the increased abundance of Parabacteroides in feces. In addition, the bile acid receptor FXR in femur was also activated in the LPN-H group, suggesting that LPN may regulate bone health through the microbiota-metabolite-bone axis. The results of this study suggest that LPN has potential application value in regulating bone metabolism during growth.

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来源期刊
Food & Function
Food & Function BIOCHEMISTRY & MOLECULAR BIOLOGY-FOOD SCIENCE & TECHNOLOGY
CiteScore
10.10
自引率
6.60%
发文量
957
审稿时长
1.8 months
期刊介绍: Food & Function provides a unique venue for physicists, chemists, biochemists, nutritionists and other food scientists to publish work at the interface of the chemistry, physics and biology of food. The journal focuses on food and the functions of food in relation to health.
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