Jiafei Qian, Chuanhong Ren, Fan Wang, Yunlin Cao, Yan Guo, Xiaoyong Zhao, Yilong Liu, Changqing Zhu, Xiaoying Li, Hongxia Xu, Junwei Chen, Kunsong Chen, Xian Li
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引用次数: 0
摘要
黄酮醇苷是植物的次生代谢产物，对植物的发育和抗UV-B辐射等胁迫具有重要作用。udp -糖基转移酶（UGT）催化黄酮醇苷生物合成的最后一步。枇杷黄酮醇苷含量丰富，但负责黄酮醇苷积累的UGTs尚不清楚。本文采用LC-MS/MS对枇杷不同组织中的13种黄酮醇苷类进行了表征，包括单糖苷类和二糖苷类。UV-B辐照显著增加了枇杷果实中4种槲皮素苷和2种山奈酚苷的积累。通过UGT基因家族分析、转录组分析、重组蛋白酶学分析以及在烟叶中的瞬时过表达分析，鉴定出3个UGT，分别为黄酮醇3- o -半乳糖基转移酶EjUGT78T4、黄酮醇3- o -葡萄糖基转移酶EjUGT78S3和黄酮醇1→6鼠李糖基转移酶EjUGT91AK7。这项工作阐明了通过ugt介导的糖基化在枇杷中形成黄酮醇苷。
Genome-wide identification of UDP-glycosyltransferases involved in flavonol glycosylation induced by UV-B irradiation in Eriobotrya japonica.
Flavonol glycosides are secondary metabolites important for plant development and stress defense such as UV-B irradiation. UDP-glycosyltransferase (UGT) catalyzes the last step in the biosynthesis of flavonol glycosides. Eriobotrya japonica is abundant in flavonol glycosides, but UGTs responsible for accumulation of flavonol glycosides remain unknown. Here, 13 flavonol glycosides including monoglycosides and diglycosides were characterized in different tissues of loquat by LC-MS/MS. UV-B irradiation significantly increased the accumulation of four quercetin glycosides and two kaempferol glycosides in loquat fruit. Based on UGT gene family analysis, transcriptome analysis, enzyme assays of recombinant proteins as well as transient overexpression assays in Nicotiana benthamiana, three UGTs were identified, i.e. EjUGT78T4 as flavonol 3-O-galactosyltransferase, EjUGT78S3 as flavonol 3-O-glucosyltransferase, and EjUGT91AK7 as flavonol 1 → 6 rhamnosyltransferase. This work elucidates the formation of flavonol glycosides in loquat through UGT-mediated glycosylation.
期刊介绍：
Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement.
Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB.
Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.
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