噪声性听力损失豚鼠耳蜗液的代谢组修饰和潜在生物标志物。

IF 1.2 4区 医学 Q3 OTORHINOLARYNGOLOGY Acta Oto-Laryngologica Pub Date : 2025-02-01 Epub Date: 2025-01-15 DOI:10.1080/00016489.2024.2445738
Guowei Qi, Jinge Tang, Handai Qin, Runnan Han, Qingqing Jiang, Ning Yu, Shiming Yang, Dongyi Han
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引用次数: 0

摘要

背景:噪声性听力损失(NIHL)是一种获得性感音神经性听力损失,近年来发病率呈上升趋势。因此,阐明确切的病理生理机制,提出有效的治疗和预防方法成为当务之急。虽然对NIHL进行了大量的研究,但很少有针对代谢物的研究。目的:揭示噪声损伤后耳蜗液代谢组学的变化,寻找NIHL的内耳生物标志物。材料与方法:本研究对脉冲噪声暴露后豚鼠耳蜗液进行GC-MS和LC-MS非靶向代谢组学分析。结果:脉冲噪声暴露后,从豚鼠耳蜗液中筛选出62种显著改变的代谢物,并选择脱氧核糖1-磷酸作为NIHL的关键代谢物和潜在生物标志物。KEGG通路分析显示,噪声后各观察时间点氧化磷酸化、甘油磷脂代谢、嘧啶代谢和戊糖磷酸途径均发生显著变化。结论及意义:本研究有效地促进了代谢组学在听力研究中的应用。内耳NIHL的病理生理过程与氧化磷酸化、甘油磷脂代谢、嘧啶代谢和戊糖磷酸途径密切相关,脱氧核糖1-磷酸可作为NIHL的生物标志物。
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Metabolome modification and underlying biomarker of noise-induced hearing loss Guinea pig cochlear fluid.

Background: Noise-induced hearing loss (NIHL) is a kind of acquired sensorineural hearing loss and has shown an increasing incidence in recent years. Hence, elucidating the exact pathophysiological mechanisms and proposing effective treatment and prevention methods become the top priority. Though a great number of researches have been carried out on NIHL, few of them were focused on metabolites.

Aims/objectives: To reveal the metabolomic changes in cochlear fluid after noise injury and search for underlying inner ear biomarkers of NIHL.

Material and methods: In this study, cochlea fluid extracted from guinea pigs after impulse noise exposure were subjected to GC-MS and LC-MS untargeted metabolomics analysis.

Results: After impulse noise exposure, 62 significantly changed metabolites in guinea pig cochlea fluid were screened out and deoxyribose 1-phosphate was selected as the key metabolite and underlying biomarker for NIHL. KEGG pathway analysis showed that oxidative phosphorylation, glycerophospholipid metabolism, pyrimidine metabolism and pentose phosphate pathway were significantly changed at all observed time points after noise.

Conclusions and significance: This study effectively promoted the application of metabolomics in hearing research. The pathophysiology process of NIHL in the inner ear was closely connected with oxidative phosphorylation, glycerophospholipid metabolism, pyrimidine metabolism and pentose phosphate pathway and deoxyribose 1-phosphate could be the biomarker for NIHL.

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来源期刊
Acta Oto-Laryngologica
Acta Oto-Laryngologica 医学-耳鼻喉科学
CiteScore
2.50
自引率
0.00%
发文量
99
审稿时长
3-6 weeks
期刊介绍: Acta Oto-Laryngologica is a truly international journal for translational otolaryngology and head- and neck surgery. The journal presents cutting-edge papers on clinical practice, clinical research and basic sciences. Acta also bridges the gap between clinical and basic research.
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