在研究中收集微生物组样本的最佳实践指南。

IF 4.8 2区 医学 Q1 UROLOGY & NEPHROLOGY European urology focus Pub Date : 2025-01-13 DOI:10.1016/j.euf.2024.12.007
Ilaha Isali, Thomas R Wong, Shari Tian
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引用次数: 0

摘要

微生物组研究有可能为微生物群落与人体之间的复杂关系提供有价值的信息。为了帮助促进将这一潜在的革命性研究转化为临床医学,有必要制定共同的指导方针和最佳做法。这些指导方针应以该领域最近研究的主要发现为基础,并解决以前发表的文献中存在差异的主要领域。基于这篇综述的发现,未来的微生物组研究应该采用一致和明确的命名方法,使用标准化的术语来减少混淆。此外,只有经过证明可以降低污染风险的样品采集方法才能被使用。虽然样品储存经常给微生物组研究带来困难,但有一些经过验证的技术可以改善结果。有了文献中广泛的研究方法,元数据收集、工具包和分析软件的标准化也可能是有益的。最后,与所有基础研究一样,文件和记录对于确保可重复性至关重要。本文综述为微生物组研究的循证指南提供了基础。患者总结:本报告概述了进行微生物组研究的一般指导方针和最佳实践,这可能会显著推进医学领域。
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Best Practice Guidelines for Collecting Microbiome Samples in Research Studies.

Microbiome research has the potential to provide valuable information regarding the complex relationship between microbial communities and the human body. To help facilitate the translation of this potentially revolutionary research to clinical medicine, common guidelines and best practices are necessary. These guidelines should be based on the key findings of recent research in the field and address the primary areas of discrepancy in the previously published literature. Based on this review's findings, future microbiome research should adopt a consistent and clear approach to nomenclature, using standardized terminology to mitigate confusion. Additionally, only sample collection methods proven to reduce the risk of contamination should be utilized. While sample storage often creates difficulty in microbiome research, there are proven techniques that can improve results. With the wide array of research methodologies in the literature, standardization of metadata collection, kits, and analysis software could also be beneficial. Finally, documentation and records are critical in ensuring reproducibility, as is the case with all basic research. This brief review provides a foundation for evidence-based guidelines in microbiome research. PATIENT SUMMARY: This report outlines general guidelines and best practices for conducting microbiome research, which could significantly advance the field of medicine.

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来源期刊
European urology focus
European urology focus Medicine-Urology
CiteScore
10.40
自引率
3.70%
发文量
274
审稿时长
23 days
期刊介绍: European Urology Focus is a new sister journal to European Urology and an official publication of the European Association of Urology (EAU). EU Focus will publish original articles, opinion piece editorials and topical reviews on a wide range of urological issues such as oncology, functional urology, reconstructive urology, laparoscopy, robotic surgery, endourology, female urology, andrology, paediatric urology and sexual medicine. The editorial team welcome basic and translational research articles in the field of urological diseases. Authors may be solicited by the Editor directly. All submitted manuscripts will be peer-reviewed by a panel of experts before being considered for publication.
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