使用低成本3D打印,可定制大规模HPLC馏分收集。

IF 2 Q3 ENGINEERING, ELECTRICAL & ELECTRONIC HardwareX Pub Date : 2024-12-10 DOI:10.1016/j.ohx.2024.e00612
William J. Crandall , Marco Caputo , Lewis Marquez , Zachery R. Jarrell , Cassandra L. Quave
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引用次数: 0

摘要

高效液相色谱(HPLC)是一种宝贵的技术,已用于分离各种分子几十年。通过分数收集系统的自动化,这些系统中洗脱液的可重复性收集得到了显着改善。目前商业上可用的馏分收集器不容易定制,与其他平台不兼容,并且具有很大的成本障碍,使许多研究人员无法使用。在这里,我们提出了一个低成本的可定制的馏分收集器,可以很容易地配对到任何高效液相色谱系统的有效建设。值得注意的是,它支持比商业替代品大得多的收集量。使用业余爱好者级三维(3D)打印机(Creality Ender 3 Pro)和铝型材,馏分收集器的建造成本不到280美元。额外的图形用户界面(GUI)支持对收集方法进行简单的编程,不需要编码经验就可以操作收集器。所提出的馏分收集器可以高度定制,并使用收集容器大470毫升(80倍),便于在预备规模上重复收集。该平台的使用将增加可扩展和迭代分数收集方法的可重复性,同时消除成本障碍,并允许高度的可定制性。
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Customizable large-scale HPLC fraction collection using low-cost 3D printing
High-performance liquid chromatography (HPLC) is an invaluable technique that has been used for many decades for the separation of various molecules. The reproducible collection of eluates from these systems has been significantly improved via its automation by fraction collection systems. Current commercially available fraction collectors are not easily customizable, incompatible with other platforms, and come with a large cost barrier making them inaccessible to many researchers. Here we present the efficient construction of a low-cost customizable fraction collector that can easily be paired to any HPLC system. Notably, it supports significantly larger volumes for collection than commercial alternatives. Using a hobbyist-grade three-dimensional (3D) printer (Creality Ender 3 Pro) and aluminum extrusions, the fraction collector can be built for less than $280 USD. An additional graphical user interface (GUI) enables simple programming of the collection methods, requiring no coding experience to operate the collector. The presented fraction collector can be highly customized and use collection vessels as large as 470 mL (80x), facilitating repeated collection at a preparatory scale. The use of this platform will increase the reproducibility of scalable and iterative fraction collection methods while removing the cost barrier and allowing for a high degree of customizability.
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来源期刊
HardwareX
HardwareX Engineering-Industrial and Manufacturing Engineering
CiteScore
4.10
自引率
18.20%
发文量
124
审稿时长
24 weeks
期刊介绍: HardwareX is an open access journal established to promote free and open source designing, building and customizing of scientific infrastructure (hardware). HardwareX aims to recognize researchers for the time and effort in developing scientific infrastructure while providing end-users with sufficient information to replicate and validate the advances presented. HardwareX is open to input from all scientific, technological and medical disciplines. Scientific infrastructure will be interpreted in the broadest sense. Including hardware modifications to existing infrastructure, sensors and tools that perform measurements and other functions outside of the traditional lab setting (such as wearables, air/water quality sensors, and low cost alternatives to existing tools), and the creation of wholly new tools for either standard or novel laboratory tasks. Authors are encouraged to submit hardware developments that address all aspects of science, not only the final measurement, for example, enhancements in sample preparation and handling, user safety, and quality control. The use of distributed digital manufacturing strategies (e.g. 3-D printing) is encouraged. All designs must be submitted under an open hardware license.
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