基于增强过氧化物酶样活性的碳点纳米酶的超灵敏比色/荧光/光热传感检测平台

IF 5.3 2区 化学 Q1 CHEMISTRY, ANALYTICAL Microchimica Acta Pub Date : 2025-01-20 DOI:10.1007/s00604-025-06961-0
Wei Wu, Chen Ni, Yan-Li Huang, Hui-Ling Cheng, Yu-Han Shi, Ya-Hui Zhang, Zhi-Hong Xu, Guo-Qi Zhang
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引用次数: 0

摘要

基于碳点纳米酶(CDszymes)的过氧化物酶样活性增强,通过d -氨基酸氧化酶的特异性氧化反应催化d -氨基酸生成H2O2,构建了唾液中d -氨基酸的超灵敏检测平台。随着d -氨基酸浓度的增加,催化产物的蓝色逐渐加深,荧光cds酶逐渐猝灭,温度逐渐升高。以d -丙氨酸为d -氨基酸模型,在比色/光热/荧光模式下,d -丙氨酸的检出限分别为0.3 μM、1.8 μM和0.04 μM。该检测平台在临床诊断中具有广阔的应用前景。这种特殊的传感性能可归因于利用了具有出色POD活性的cds酶。围绕盲合成具有高效POD活性的cds酶,本研究深入探讨了前体官能团调控cds酶POD活性的潜在机制。本研究探讨了价带理论增强cds酶的过氧化物酶样,从而为cds酶的设计提供了一种合理的方法。图形抽象
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An ultrasensitive colorimetric/fluorescent/photothermal sensing platform for the detection of D-amino acids based on carbon dots nanozymes with enhanced peroxidase-like activity

Based on the enhanced peroxidase-like activity of carbon dots nanozymes (CDszymes), with a specific oxidation reaction of D-amino acid oxidase catalysing the formation of H2O2 from D-amino acid, an ultrasensitive sensing platform, was constructed for the quantitative detection of D-amino acids in saliva. With the increase of D-amino acids concentration, the blue color of catalytic product gradually deepend, the fluorescence CDszymes gradually quenched, and the temperature gradually increased. Using D-alanine as D-amino acid models, the detection limits of D-alanine in colorimetric/photothermal/fluorescent mode were 0.3 μM, 1.8 μM, and 0.04 μM, respectively. The proposed detection platform exhibits promising application potential in clinical diagnostics. The exceptional sensing performance can be attributed to the utilization of CDszymes with outstanding POD activity. Revolving around the blind synthesis of CDszymes exhibiting high-efficiency POD activity, this study delved into the underlying mechanism governing the regulation of the POD activity of CDszymes by precursor functional groups. This work investigates the valence band theory to enhance the peroxidase-like of CDszymes, thereby offering a rational approach for designing CDszymes.

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来源期刊
Microchimica Acta
Microchimica Acta 化学-分析化学
CiteScore
9.80
自引率
5.30%
发文量
410
审稿时长
2.7 months
期刊介绍: As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.
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