{"title":"基于增强过氧化物酶样活性的碳点纳米酶的超灵敏比色/荧光/光热传感检测平台","authors":"Wei Wu, Chen Ni, Yan-Li Huang, Hui-Ling Cheng, Yu-Han Shi, Ya-Hui Zhang, Zhi-Hong Xu, Guo-Qi Zhang","doi":"10.1007/s00604-025-06961-0","DOIUrl":null,"url":null,"abstract":"<div><p>Based on the enhanced peroxidase-like activity of carbon dots nanozymes (CDszymes), with a specific oxidation reaction of D-amino acid oxidase catalysing the formation of H<sub>2</sub>O<sub>2</sub> from D-amino acid, an ultrasensitive sensing platform, was constructed for the quantitative detection of D-amino acids in saliva. With the increase of D-amino acids concentration, the blue color of catalytic product gradually deepend, the fluorescence CDszymes gradually quenched, and the temperature gradually increased. Using D-alanine as D-amino acid models, the detection limits of D-alanine in colorimetric/photothermal/fluorescent mode were 0.3 μM, 1.8 μM, and 0.04 μM, respectively. The proposed detection platform exhibits promising application potential in clinical diagnostics. The exceptional sensing performance can be attributed to the utilization of CDszymes with outstanding POD activity. Revolving around the blind synthesis of CDszymes exhibiting high-efficiency POD activity, this study delved into the underlying mechanism governing the regulation of the POD activity of CDszymes by precursor functional groups. This work investigates the valence band theory to enhance the peroxidase-like of CDszymes, thereby offering a rational approach for designing CDszymes.</p><h3>Graphical abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 2","pages":""},"PeriodicalIF":5.3000,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"An ultrasensitive colorimetric/fluorescent/photothermal sensing platform for the detection of D-amino acids based on carbon dots nanozymes with enhanced peroxidase-like activity\",\"authors\":\"Wei Wu, Chen Ni, Yan-Li Huang, Hui-Ling Cheng, Yu-Han Shi, Ya-Hui Zhang, Zhi-Hong Xu, Guo-Qi Zhang\",\"doi\":\"10.1007/s00604-025-06961-0\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Based on the enhanced peroxidase-like activity of carbon dots nanozymes (CDszymes), with a specific oxidation reaction of D-amino acid oxidase catalysing the formation of H<sub>2</sub>O<sub>2</sub> from D-amino acid, an ultrasensitive sensing platform, was constructed for the quantitative detection of D-amino acids in saliva. With the increase of D-amino acids concentration, the blue color of catalytic product gradually deepend, the fluorescence CDszymes gradually quenched, and the temperature gradually increased. Using D-alanine as D-amino acid models, the detection limits of D-alanine in colorimetric/photothermal/fluorescent mode were 0.3 μM, 1.8 μM, and 0.04 μM, respectively. The proposed detection platform exhibits promising application potential in clinical diagnostics. The exceptional sensing performance can be attributed to the utilization of CDszymes with outstanding POD activity. Revolving around the blind synthesis of CDszymes exhibiting high-efficiency POD activity, this study delved into the underlying mechanism governing the regulation of the POD activity of CDszymes by precursor functional groups. This work investigates the valence band theory to enhance the peroxidase-like of CDszymes, thereby offering a rational approach for designing CDszymes.</p><h3>Graphical abstract</h3>\\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>\",\"PeriodicalId\":705,\"journal\":{\"name\":\"Microchimica Acta\",\"volume\":\"192 2\",\"pages\":\"\"},\"PeriodicalIF\":5.3000,\"publicationDate\":\"2025-01-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microchimica Acta\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s00604-025-06961-0\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microchimica Acta","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s00604-025-06961-0","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
An ultrasensitive colorimetric/fluorescent/photothermal sensing platform for the detection of D-amino acids based on carbon dots nanozymes with enhanced peroxidase-like activity
Based on the enhanced peroxidase-like activity of carbon dots nanozymes (CDszymes), with a specific oxidation reaction of D-amino acid oxidase catalysing the formation of H2O2 from D-amino acid, an ultrasensitive sensing platform, was constructed for the quantitative detection of D-amino acids in saliva. With the increase of D-amino acids concentration, the blue color of catalytic product gradually deepend, the fluorescence CDszymes gradually quenched, and the temperature gradually increased. Using D-alanine as D-amino acid models, the detection limits of D-alanine in colorimetric/photothermal/fluorescent mode were 0.3 μM, 1.8 μM, and 0.04 μM, respectively. The proposed detection platform exhibits promising application potential in clinical diagnostics. The exceptional sensing performance can be attributed to the utilization of CDszymes with outstanding POD activity. Revolving around the blind synthesis of CDszymes exhibiting high-efficiency POD activity, this study delved into the underlying mechanism governing the regulation of the POD activity of CDszymes by precursor functional groups. This work investigates the valence band theory to enhance the peroxidase-like of CDszymes, thereby offering a rational approach for designing CDszymes.
期刊介绍:
As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.