Dalia E Rodríguez-Torres, Anilu M Saucedo-Sariñana, Alejandra Palacios-Ramírez, Miriam Y Godínez-Rodríguez, Efraín Salas-González, Aldo A Alcaraz-Wong, José E García-Ortiz, Mónica A Rosales-Reynoso
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引用次数: 0
摘要
背景：循环游离DNA （cfDNA）、核DNA （nDNA）和线粒体DNA （mtDNA）作为癌症潜在生物标志物的有效性仍存在争议。目的：测定乳腺癌（BC）患者血浆cfDNA、nDNA和mtDNA水平。材料和方法：本研究共纳入86名女性（69名BC患者和17名对照组）。采用荧光法测定cfDNA，采用实时聚合酶链反应（qPCR）测定mtDNA和nDNA水平。结果：BC患者cfDNA浓度高于对照组（p = 0.001）。50岁以上的患者cfDNA浓度较高（p = 0.001）。CM患者的nDNA水平高于对照组（p = 0.004）。mtDNA水平差异无统计学意义（p < 0.05）。cfDNA和nDNA的浓度具有显著的区分力（AUC = 0.968, CI = 0.93-1.00, p = 0.001）；AUC = 0.724, CI = 0.59 ~ 0.85, p = 0.004。结论：cfDNA浓度及cfDNA与nDNA联合检测是诊断BC的有效生物标志物。
Analysis of circulating cell-free nuclear and mitochondrial DNA in plasma of Mexican patients with breast cancer.
Background: The usefulness of circulating free DNA (cfDNA), nuclear DNA (nDNA) and mitochondrial DNA (mtDNA) as potential biomarkers in cancer remains controversial.
Objective: To determine the concentration of cfDNA and plasma nDNA and mtDNA levels in breast cancer (BC) patients.
Material and methods: This study included a total of 86 women (69 patients with BC and 17 women as a control group). The cfDNA was determined using fluorometry, and the levels of mtDNA and nDNA were quantified using real-time polymerase chain reaction (qPCR).
Results: The concentration of cfDNA was higher in patients with BC compared to the control group (p = 0.001). A higher concentration of cfDNA was also associated with patients over 50 years old (p = 0.001). The level of nDNA was higher in patients with CM compared to the control group (p = 0.004). Levels of mtDNA did not show significant differences (p > 0.005). The concentration of cfDNA and nDNA showed significant discriminatory power (AUC = 0.968, CI = 0.93-1.00, p = 0.001; and AUC = 0.724, CI = 0.59-0.85, p = 0.004, respectively.
Conclusions: The concentration of cfDNA and the combination of cfDNA and nDNA are useful biomarkers for diagnosing patients with BC.
期刊介绍：
Gaceta Médica de México México is the official scientific journal of the Academia Nacional de Medicina de México, A.C. Its goal is to contribute to health professionals by publishing the most relevant progress both in research and clinical practice.
Gaceta Médica de México is a bimonthly peer reviewed journal, published both in paper and online in open access, both in Spanish and English. It has a brilliant editorial board formed by national and international experts.
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