利用多元单细胞拉曼光谱研究右旋糖酐包被氧化铈纳米颗粒对肺成纤维细胞的影响。

IF 3.6 3区 医学 Q3 NANOSCIENCE & NANOTECHNOLOGY Nanotoxicology Pub Date : 2025-01-22 DOI:10.1080/17435390.2025.2453576
Mirjana Mićević, Sonja Čalija, Lela Korićanac, Jelena Žakula, Aleksandra Vilotić, Marko Radović, Igor Golić, Aleksandra Korać, Mirjana Nacka-Aleksić, Bojan Stojadinović, Zorana Dohčević-Mitrović
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引用次数: 0

摘要

在这项研究中,我们研究了高可溶性葡聚糖包被的CeO2纳米颗粒对人胎儿肺成纤维细胞MRC-5的细胞毒性作用。我们通过拉曼光谱检查了单个纳米颗粒处理的细胞,并使用非负主成分分析和k-means聚类分析了拉曼光谱。通过这种方式,我们确定了处理细胞之间的剂量依赖性差异,这种差异通过脂质、磷脂和rna相关拉曼模式的强度变化来反映。通过对细胞生长、活力和诱导凋亡进行标准生物学测试,这些变化与纳米颗粒诱导的凋亡过程相关。还检测到具有特定光谱特征的细胞,即非凋亡但可能是自噬细胞死亡模式的细胞。此外,拉曼成像结合主成分和顶点成分分析来绘制生物分子在处理和未处理细胞中的空间分布。这项工作提供了根据葡聚糖包被CeO2纳米颗粒剂量的不同处理细胞的不同结果状态的描述,这可以在稍后用于工业或医疗应用的纳米颗粒设计中使用。单细胞拉曼光谱产生的广泛信息内容有可能检测由纳米颗粒引起的生化变化,否则将需要一系列昂贵且耗时的标准生物技术。
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Probing the effects of dextran-coated CeO2 nanoparticles on lung fibroblasts using multivariate single-cell Raman spectroscopy.

In this study, we investigated the cytotoxic effect of highly soluble dextran-coated CeO2 nanoparticles on human fetal lung fibroblasts MRC-5. We examined individual nanoparticle-treated cells by Raman spectroscopy and analyzed Raman spectra using non-negative principal component analysis and k-means clustering. In this way, we determined dose-dependent differences between treated cells, which were reflected through the intensity change of lipid, phospholipid and RNA-related Raman modes. Performing standard biological tests for cell growth, viability and induction of apoptosis in parallel, these changes were correlated with nanoparticle-induced apoptotic processes. The cells with specific spectral characteristics, referring to non-apoptotic, but possibly autophagic cell death modality, were also detected. Additionally, Raman imaging combined with principal component and vertex component analysis was used to map the spatial distribution of biological molecules in treated and untreated cells. This work provided the description of different resulting states of the treated cells depending on the dextran-coated CeO2 nanoparticles dose, which can be later used in the design of the nanoparticles for industrial or medical applications. The wide content of information resulting from single-cell Raman spectroscopy has the potential to detect biochemical changes caused by nanoparticles that would otherwise require a series of expensive and time-consuming standard biological techniques.

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来源期刊
Nanotoxicology
Nanotoxicology 医学-毒理学
CiteScore
10.10
自引率
4.00%
发文量
45
审稿时长
3.5 months
期刊介绍: Nanotoxicology invites contributions addressing research relating to the potential for human and environmental exposure, hazard and risk associated with the use and development of nano-structured materials. In this context, the term nano-structured materials has a broad definition, including ‘materials with at least one dimension in the nanometer size range’. These nanomaterials range from nanoparticles and nanomedicines, to nano-surfaces of larger materials and composite materials. The range of nanomaterials in use and under development is extremely diverse, so this journal includes a range of materials generated for purposeful delivery into the body (food, medicines, diagnostics and prosthetics), to consumer products (e.g. paints, cosmetics, electronics and clothing), and particles designed for environmental applications (e.g. remediation). It is the nano-size range if these materials which unifies them and defines the scope of Nanotoxicology . While the term ‘toxicology’ indicates risk, the journal Nanotoxicology also aims to encompass studies that enhance safety during the production, use and disposal of nanomaterials. Well-controlled studies demonstrating a lack of exposure, hazard or risk associated with nanomaterials, or studies aiming to improve biocompatibility are welcomed and encouraged, as such studies will lead to an advancement of nanotechnology. Furthermore, many nanoparticles are developed with the intention to improve human health (e.g. antimicrobial agents), and again, such articles are encouraged. In order to promote quality, Nanotoxicology will prioritise publications that have demonstrated characterisation of the nanomaterials investigated.
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