5-羟色胺在肠期旋毛虫排出中的作用。

IF 2 2区 农林科学 Q2 PARASITOLOGY Veterinary parasitology Pub Date : 2025-01-13 DOI:10.1016/j.vetpar.2025.110396
Tongxuan Zhang, Zhiyu Zhu, Zhiyuan Zhang, Xueting Wang, Xuanrui Liu, Wentao Yang, Xin Cao, Yanlong Jiang, Jianzhong Wang, Yan Zeng, Chunwei Shi, Haibin Huang, Chunfeng Wang, Nan Wang, Guilian Yang
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引用次数: 0

摘要

在旋毛虫感染的肠道阶段,它可以刺激宿主的肠道蠕动排出蠕虫。5-羟色胺(5-HT)是一种调节肠平滑肌收缩的神经递质。IL-33特异性结合ST2受体,促进肠嗜铬细胞分泌5-HT。然而,在螺旋体感染的肠道阶段,宿主是否能够通过IL-33-ST2信号通路调节5-HT的分泌来驱逐蠕虫,目前尚不清楚。因此,我们将ST2抑制剂iST2应用于螺旋体感染小鼠模型和MODE-K细胞,分析IL-33-ST2信号通路在螺旋体感染肠道期5-HT分泌中的作用。结果表明,在螺旋体感染肠期,ST2、IL-33和TPH1(5-HT生物合成的限制性酶-色氨酸羟化酶1)在小肠中的表达升高。同时,小肠分泌的5-HT和IL-33水平显著升高。经iST2处理后,5-HT水平显著降低,导致驱虫能力下降。经iST2处理后螺旋螺旋体的排泄产物处理的MODE-K细胞中5-HT水平下降。上述结果表明,IL-33-ST2信号通路可能在螺旋体肠期促进5-HT分泌,增强肠道驱虫能力方面发挥重要作用。
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The role of 5-hydroxytryptamine on expulsion of Trichinella spiralis during the intestinal stage.

During the intestinal stage of Trichinella spiralis (T. spiralis) infection, it can stimulate host's intestinal peristalsis to expulse worms. 5-hydroxytryptamine (5-HT) is a neurotransmitter which can regulate the contraction of intestinal smooth muscle. IL-33 specifically binds to ST2 receptor to promote the secretion of 5-HT by intestinal enterochromaffin cells. However, it remains unclear whether the host is able to modulate the secretion of 5-HT to expulse worms through the IL-33-ST2 signaling pathway during the intestinal stage of T. spiralis infection. Therefore, ST2 inhibitor iST2 was used in a T. spiralis infected mouse model and MODE-K cells to analyze the role of IL-33-ST2 signaling pathway in the secretion of 5-HT during the intestinal phase of T. spiralis infection. The results indicated that the expression of ST2, IL-33, and TPH1(tryptophan hydroxylase 1, the rate-limiting enzyme in the biosynthesis of 5-HT) in the small intestine were increased during the intestinal phase of T. spiralis infection. Meanwhile the levels of secretory 5-HT and IL-33 in the small intestine were significantly increased. After iST2 treatment, the level of 5-HT was significantly decreased, resulting in diminished worms expulsion capability. The decrease of 5-HT was observed in MODE-K cells treated with excretory-secretory products of T. spiralis post iST2 treatment. The above results demonstrated that IL-33-ST2 signaling pathway might play a crucial role in promoting the secretion of 5-HT which enhancing the ability of the intestine to expulse worms during the intestinal stage of T. spiralis.

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来源期刊
Veterinary parasitology
Veterinary parasitology 农林科学-寄生虫学
CiteScore
5.30
自引率
7.70%
发文量
126
审稿时长
36 days
期刊介绍: The journal Veterinary Parasitology has an open access mirror journal,Veterinary Parasitology: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. This journal is concerned with those aspects of helminthology, protozoology and entomology which are of interest to animal health investigators, veterinary practitioners and others with a special interest in parasitology. Papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites in all domesticated animals, fall within the scope of the journal. Papers of geographically limited (local) interest which are not of interest to an international audience will not be accepted. Authors who submit papers based on local data will need to indicate why their paper is relevant to a broader readership. Parasitological studies on laboratory animals fall within the scope of the journal only if they provide a reasonably close model of a disease of domestic animals. Additionally the journal will consider papers relating to wildlife species where they may act as disease reservoirs to domestic animals, or as a zoonotic reservoir. Case studies considered to be unique or of specific interest to the journal, will also be considered on occasions at the Editors'' discretion. Papers dealing exclusively with the taxonomy of parasites do not fall within the scope of the journal.
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