Haoqi Li , Zijie Cheng , Huimin Li , Yun Yin , Yuqing Li , Tao Chen , Xin Dong , Qingxun Hu , Dan Wu
{"title":"一种新的遗传编码指标，用于破译活细胞中的细胞质和线粒体一氧化氮。","authors":"Haoqi Li , Zijie Cheng , Huimin Li , Yun Yin , Yuqing Li , Tao Chen , Xin Dong , Qingxun Hu , Dan Wu","doi":"10.1016/j.bbrc.2025.151345","DOIUrl":null,"url":null,"abstract":"<div><div>Nitric oxide (NO) has been highlighted as a key gaseous signaling molecule in the body, playing a central role in various physiological and pathological processes. However, a comprehensive analysis of NO metabolism dynamics in living cells remains a significant challenge. To address this, we have developed and characterized a novel genetically encoded NO fluorescence sensor, GefiNO, to investigate NO metabolism dynamics in living cells and subcellular organelles. In the study, we described the protein detection capabilities and subcellular spatiotemporal localization of GefiNO sensors. By analyzing NO metabolic responses in macrophages of different subtypes, we provide a comprehensive view of NO dynamics at the cellular level. The GefiNO sensor represents a valuable tool for monitoring extensive NO dynamics in living cells and offers potential for uncovering new insights into cellular metabolic mechanisms.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"749 ","pages":"Article 151345"},"PeriodicalIF":2.2000,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A novel genetically encoded indicator for deciphering cytosolic and mitochondrial nitric oxide in live cells\",\"authors\":\"Haoqi Li , Zijie Cheng , Huimin Li , Yun Yin , Yuqing Li , Tao Chen , Xin Dong , Qingxun Hu , Dan Wu\",\"doi\":\"10.1016/j.bbrc.2025.151345\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Nitric oxide (NO) has been highlighted as a key gaseous signaling molecule in the body, playing a central role in various physiological and pathological processes. However, a comprehensive analysis of NO metabolism dynamics in living cells remains a significant challenge. To address this, we have developed and characterized a novel genetically encoded NO fluorescence sensor, GefiNO, to investigate NO metabolism dynamics in living cells and subcellular organelles. In the study, we described the protein detection capabilities and subcellular spatiotemporal localization of GefiNO sensors. By analyzing NO metabolic responses in macrophages of different subtypes, we provide a comprehensive view of NO dynamics at the cellular level. The GefiNO sensor represents a valuable tool for monitoring extensive NO dynamics in living cells and offers potential for uncovering new insights into cellular metabolic mechanisms.</div></div>\",\"PeriodicalId\":8779,\"journal\":{\"name\":\"Biochemical and biophysical research communications\",\"volume\":\"749 \",\"pages\":\"Article 151345\"},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2025-02-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochemical and biophysical research communications\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0006291X25000592\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/1/19 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochemical and biophysical research communications","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0006291X25000592","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/19 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
A novel genetically encoded indicator for deciphering cytosolic and mitochondrial nitric oxide in live cells
Nitric oxide (NO) has been highlighted as a key gaseous signaling molecule in the body, playing a central role in various physiological and pathological processes. However, a comprehensive analysis of NO metabolism dynamics in living cells remains a significant challenge. To address this, we have developed and characterized a novel genetically encoded NO fluorescence sensor, GefiNO, to investigate NO metabolism dynamics in living cells and subcellular organelles. In the study, we described the protein detection capabilities and subcellular spatiotemporal localization of GefiNO sensors. By analyzing NO metabolic responses in macrophages of different subtypes, we provide a comprehensive view of NO dynamics at the cellular level. The GefiNO sensor represents a valuable tool for monitoring extensive NO dynamics in living cells and offers potential for uncovering new insights into cellular metabolic mechanisms.
期刊介绍：
Biochemical and Biophysical Research Communications is the premier international journal devoted to the very rapid dissemination of timely and significant experimental results in diverse fields of biological research. The development of the "Breakthroughs and Views" section brings the minireview format to the journal, and issues often contain collections of special interest manuscripts. BBRC is published weekly (52 issues/year).Research Areas now include: Biochemistry; biophysics; cell biology; developmental biology; immunology
; molecular biology; neurobiology; plant biology and proteomics
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