Lung seven transmembrane receptors are involved in Arabidopsis root growth mediated by Danger-associated peptide Pep1

Lung seven transmembrane receptor family is a small part of Arabidopsis gene family. So far, the function of some members of the this family is unknown. Plant elicitor peptide1 (Pep1) is one of damage-associated molecular patterns (DAMPs), which could trigger root growth inhibition and plant immunity responses. Here, we identified members of the Lung seven transmembrane family, and proved they are important for Pep1-induced root growth and development. We found that the expression levels of 7TM2 and 7TM6 were elevated in wild-type treated with Pep1. Phenotypic analysis showed that the growth phenotypes of 7tm2 and 7tm6 were similar to the wild-type under Pep1 treatment, but the 7tm2 7tm6 had a Pep1 hypersensitivity phenotype compared to wild-type. Furthermore, the complementation lines were able to restore the Pep1 phenotype of 7tm2 7tm6 to that similar to wild-type. These results suggest that 7TM2 and 7TM6 are involved in the regulation of root growth by Pep1. This study revealed the new functions of the Lung seven transmembrane receptor family and provided new ideas for further revealing the molecular mechanism of Pep1-regulated root growth and development.