Fungal endophytes modulate the negative effects induced by Persistent Organic Pollutants in the antarctic plant Colobanthus quitensis.

Antarctica has one of the most sensitive ecosystems to the negative effects of Persistent Organic Pollutants (POPs) on its biodiversity. This is because of the lower temperatures and the persistence of POPs that promote their accumulation or even biomagnification. However, the impact of POPs on vascular plants is unknown. Moreover, fungal symbionts could modulate the effects on host plants to cope with this stress factor. This study investigates the molecular and ecophysiological responses of the Sub-Antarctic and Antarctic plant Colobanthus quitensis to POPs in different populations along a latitudinal gradient (53°- 67° S), emphasizing the role of endophytic fungi. The results show that exposure of POPs in C. quitensis generates oxidative stress and alters its ecophysiological performance. Nevertheless, C. quitensis in association with fungal endophytes and POPs exposure, shows lower lipid peroxidation, higher proline content and higher photosynthetic capacity, as well as higher biomass and survival percentage, compared to plants in the absence of fungal endophytes. On the other hand, the antarctic plant population (67°S) with endophytic fungi presents better stress modulating upon POPs exposure. Endophytic fungi would be more necessary for plant performance towards higher latitudes with extreme conditions, contributing significantly to their general functional adaptation. We develop a transcriptomics analyses n the C. quitensis-fungal endophytes association from the Peninsula population. We observed that fungal endophytes promote tolerance to POPs stress through upregulated genes for the redox regulation based on ascorbate and scavenging mechanisms (peroxidases, MDAR, VTC4, CCS), transformation (monooxygenases) and conjugation of compounds or metabolites (glutathione transferases, glycosyltransferases, S-transferases), and the storage or elimination of conjugates (ABC transporters, C and G family) that contribute to detoxification cell. This work highlights the contribution of endophytic fungi to plant resistance in situations of environmental stress, especially in extreme conditions such as in antarctica exposed to anthropogenic impact. The implications of these findings are relevant for the biosecurity of one of the last pristine bastions worldwide.