ZmNPF7.10决定了玉米中钾、氮从节点到叶片的分布

IF 8.1 1区生物学 Q1 PLANT SCIENCES New Phytologist Pub Date : 2025-01-30 DOI:10.1111/nph.20422

Yingying Hu, Man Zhang, Kangqi Wang, Peipei Tan, Si Jing, Wu Han, Shuwei Wang, Kaina Zhang, Xiaoming Zhao, Xiaohong Yang, Yi Wang

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引用次数: 0

摘要

在禾本科植物中，节点在养分分配中起着至关重要的作用，特别是在向发育中的叶片和生殖器官优先分配养分方面。然而，这种分布背后的分子机制仍然知之甚少。在这项研究中，我们发现了一个名为ZmNPF7.10的转运蛋白，它参与了玉米节点中钾(K)和氮(N)的分配。在爪蟾卵母细胞中，ZmNPF7.10以ph依赖的方式显示NO3−和K+转运活性。ZmNPF7.10主要表达于生殖生长期的茎节，优先表达于茎节增大维管束（EVBs）的木质部薄壁细胞。ZmNPF7.10的破坏导致玉米叶片中K和N的下降，但在节点中K和N的积累，说明ZmNPF7.10在玉米中引导K和N从节点到叶片的分配。我们在启动子区域发现了一个7.1 kb InDel的自然变异，该变异与ZmNPF7.10在节点中的转录水平、叶片K和N浓度以及籽粒产量显著相关。这些发现表明ZmNPF7.10作为一个双重转运体，在节点中介导K和N的分布。该研究为玉米养分分布的分子机制提供了重要的见解。

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ZmNPF7.10 confers potassium and nitrogen distribution from node to leaf in maize

In graminaceous plants, nodes play vital roles in nutrient allocation, especially for preferential nutrient distribution to developing leaves and reproductive organs. However, the molecular mechanisms underlying this distribution remain poorly understood.
In this study, we identified a transporter named ZmNPF7.10 that is involved in potassium (K) and nitrogen (N) distribution in maize nodes. In Xenopus oocytes, ZmNPF7.10 showed NO₃⁻ and K⁺ transport activity in a pH-dependent manner. ZmNPF7.10 is predominantly expressed in the nodes at the reproductive growth stage, and preferentially expressed in the xylem parenchyma cells of enlarged vascular bundles (EVBs) in nodes. Disruption of ZmNPF7.10 resulted in the decline of K and N in leaves, but accumulation of K and N in nodes, suggesting ZmNPF7.10 conducts K and N distribution from nodes to leaves in maize.
We identified a natural variant of 7.1-kb InDel in the promoter region that was significantly associated with ZmNPF7.10 transcript level in nodes, leaf K and N concentration, as well as grain yield.
These findings demonstrate that ZmNPF7.10 functions as a dual role transporter that mediates K and N distribution in nodes. This study provides important insights into the molecular mechanisms of nutrient distribution in maize.

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来源期刊

New Phytologist 生物-植物科学

自引率

5.30%

发文量

728

期刊介绍： New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.

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