Effective detection of kanamycin residues by target recycling-based enzyme-free signal dual amplification electrochemical aptasensor

Abuse of kanamycin is still present in livestock and poultry farming, thereby causing serious damage to the human body through food chain. Therefore, it is necessary to effectively detect kanamycin in animal sourced food. Here, four different versions of electrochemical aptasensors were developed for efficient detection of kanamycin; especially, a target recycling-based enzyme-free signal dual amplification electrochemical aptasensor (i.e., named as Ver. 4 aptasensor) targeting kanamycin was developed. Under the optimized conditions, the Ver. 4 aptasensor exhibited a high sensitivity toward kanamycin with a limit of detection (LOD) of 0.10 ng/mL (linear range from 0.48 to 4844.99 ng/mL), and a high selectivity against the kanamycin’s structural analogs. Meanwhile, good reusability and excellent detection repeatability further indicated the application feasibility of the Ver. 4 aptasensor. Moreover, the recovery rate of kanamycin from spiked milk samples ranged from 93.24 % to 103.80 %. Results presented here suggested the application potential of the Ver. 4 aptasensor for effectively detecting kanamycin in animal sourced foods.