利用SSR标记探索杏(Prunus armeniaca L.)居群的遗传多样性

IF 2.7 3区 生物学 Q2 PLANT SCIENCES South African Journal of Botany Pub Date : 2025-02-01 Epub Date: 2024-12-03 DOI:10.1016/j.sajb.2024.11.030
Bunty Shylla , Dharam Paul Sharma , Anil Handa , Manisha Thakur , Pooja Sharma , Narender Negi
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引用次数: 0

摘要

利用SSR标记对喜马偕尔邦中山杏(Prunus armeniaca L.)种质资源的遗传多样性进行了研究。杏是一种以其高营养价值而闻名的果树,为了有效的育种和保护工作,需要进行遗传多样性分析。46个SSR标记共检测到974个等位基因,平均每个位点有33.59个等位基因,PIC值在0.16 ~ 0.50之间,多态性水平较高。15个本土植物和“Shakarpara”的PCR产物显示出独特的条带,并进行BLAST测序,显示出与李子高度同源性。杏的高质量参考基因组仍然不可用,从而限制了遗传背景的表型关联的阐明。总之,本研究为了解杏种质资源的遗传多样性提供了有价值的见解,对可持续育种策略至关重要,并且需要整合先进的基因组工具来进一步阐明杏的遗传结构。
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Exploring genetic diversity in apricot (Prunus armeniaca L.) populations using SSR markers
The genetic diversity of apricot (Prunus armeniaca L.) germplasm in mid-hills of Himachal Pradesh was investigated using Simple Sequence Repeats (SSR) markers. Apricot, a fruit-bearing tree known for its high nutritional value, requires genetic diversity analysis for effective breeding and conservation efforts. Molecular characterization using 46 SSR markers produced a total of 974 alleles with an average of 33.59 alleles per locus and PIC values ranging from 0.16 to 0.50, indicating a high level of polymorphism. PCR products of 15 indigenous collections and ‘Shakarpara’ revealed unique bands that were sequenced and subjected to BLAST, showing a high degree of homology with Prunus dulcis. A high quality reference genome for apricot is still unavailable, thereby limiting the elucidation of phenotypic associations with genetic background. Overall, this study provides valuable insights into the genetic diversity of apricot germplasm, crucial for sustainable breeding strategies, and need for integrating advanced genomic tools to further elucidate the genetic architecture of apricot.
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来源期刊
South African Journal of Botany
South African Journal of Botany 生物-植物科学
CiteScore
5.20
自引率
9.70%
发文量
709
审稿时长
61 days
期刊介绍: The South African Journal of Botany publishes original papers that deal with the classification, biodiversity, morphology, physiology, molecular biology, ecology, biotechnology, ethnobotany and other botanically related aspects of species that are of importance to southern Africa. Manuscripts dealing with significant new findings on other species of the world and general botanical principles will also be considered and are encouraged.
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