在COVID-19中，帕金森病相关蛋白帕金森氏蛋白的血清水平特异性升高

IF 1.4 Q4 INFECTIOUS DISEASES Journal of clinical virology plus Pub Date : 2025-02-01 Epub Date: 2025-01-27 DOI:10.1016/j.jcvp.2025.100206

Nadezhda G. Gumanova, Natalya L. Bogdanova, Alexander Yu. Gorshkov

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引用次数: 0

摘要

冠状病毒病2019 （COVID-19）是由严重急性呼吸综合征冠状病毒2 （SARS-CoV-2）引起的，已知会导致各种不利影响，包括神经退行性疾病。本研究的目的是评估与COVID-19特异性相关的比较血清蛋白谱。方法检测2019-2021年采集的患者血清中针对SARS-CoV-2 S1蛋白受体结合域的免疫球蛋白G (IgG)-SARS抗体，确认感染。分析了2016-2018年COVID-19大流行发病前采集的血清样本中腺病毒IgG抗体（IgG- adv）。在igg - sars阳性或阴性的匹配血清样本中，使用包括两个重复的1358种特异性抗体的Signaling Explorer Antibody Array （SET100）进行比较蛋白谱分析。该分析确定Parkin是区分sars阳性和阴性状态的顶级蛋白。在2019- 2021年招募的参与者的血清中使用内部ELISA对结果进行了验证。在IgG-AdV抗体阳性或阴性的血清样本中检测对另一种病毒感染的特异性。结果血清中高水平的帕金森病相关蛋白Parkin与sars阳性状态相关(P <；0.05)，但与igg - adv阳性状态无关(P >；0.05)。结论帕金蛋白与COVID-19的特异性关联有助于识别与COVID-19效应相关的信号通路。

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Serum levels of the Parkinson's disease-linked protein Parkin are specifically elevated in COVID-19

Background

Coronavirus disease 2019 (COVID-19) is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and is known to cause various unfavorable effects, including neurodegenerative disorders. The aim of the present study was to assess comparative serum protein profiles specifically associated with COVID-19.

Methods

COVID-19 infection was confirmed by the detection of immunoglobulin G (IgG)-SARS antibodies against SARS-CoV-2 S1 protein receptor-binding domain in serum samples collected in 2019–2021. IgG antibodies to adenovirus (IgG-AdV) were analyzed in serum samples collected in 2016–2018 prior the onset of the COVID-19 pandemic. Comparative protein profiling was conducted in matched serum samples with positive or negative IgG-SARS-status using the Signaling Explorer Antibody Array (SET100) that included 1358 specific antibodies in two replicates. This analysis identified Parkin as a top protein discriminating between the SARS-positive and negative status. The results were validated using in-house ELISA in the serum of participants recruited in 2019- 2021. Specificity versus another viral infection was tested in the serum samples with positive or negative status of IgG-AdV antibodies.

Results

High serum levels of the Parkinson's disease-linked protein Parkin were associated with SARS-positive status (P < 0.05) but were not associated with IgG-AdV-positive-status (P > 0.05).