Qinyan Cai , Min Chen , Zhendi Wang , Xuan Xu , Tingting Zheng
{"title":"海胆样Bi2S3@Co3O4复合材料表面增强拉曼散射探针用于神经干细胞分化过程中多巴胺的检测","authors":"Qinyan Cai , Min Chen , Zhendi Wang , Xuan Xu , Tingting Zheng","doi":"10.1016/j.saa.2025.125845","DOIUrl":null,"url":null,"abstract":"<div><div>Dopamine (DA) is a significant neurotransmitter involved in various functions of the central nervous system, containing the regulation of motor functions, arousal, motivation and reward processing. Therefore, precise measurement of DA concentrations is vital for studying different dopaminergic neural pathways and for the diagnosis of neurological disorders. Herein, we developed a new semiconductor surface-enhanced Raman spectroscopic (SERS) platform designed for the real-time monitoring DA release from live cells with high selectivity and sensitivity. The sea urchin-like Bi<sub>2</sub>S<sub>3</sub>@Co<sub>3</sub>O<sub>4</sub> composite substrate exhibited not only a wide linear range from 1.00 to 1.00 × 10<sup>3</sup> nmol/L but also a limit of detection (LOD) as low as 0.92 nmol/L for Methylene Blue (MB) molecules. In addition, we monitored DA release during the differentiation of neural stem cells (NSCs) into dopaminergic neurons by virtue of our constructed SERS platform in a nondestructive and in situ manner. Consequently, the developed SERS platform facilitates the direct detection of exocytotic DA from neuronal cells, enabling real-time assessment of cell viability in models of neurodegenerative disease-related cellular damage.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"332 ","pages":"Article 125845"},"PeriodicalIF":4.6000,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Surface-enhanced Raman scattering probes based on sea urchin-like Bi2S3@Co3O4 composite for the detection of dopamine during neural stem cell differentiation\",\"authors\":\"Qinyan Cai , Min Chen , Zhendi Wang , Xuan Xu , Tingting Zheng\",\"doi\":\"10.1016/j.saa.2025.125845\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Dopamine (DA) is a significant neurotransmitter involved in various functions of the central nervous system, containing the regulation of motor functions, arousal, motivation and reward processing. Therefore, precise measurement of DA concentrations is vital for studying different dopaminergic neural pathways and for the diagnosis of neurological disorders. Herein, we developed a new semiconductor surface-enhanced Raman spectroscopic (SERS) platform designed for the real-time monitoring DA release from live cells with high selectivity and sensitivity. The sea urchin-like Bi<sub>2</sub>S<sub>3</sub>@Co<sub>3</sub>O<sub>4</sub> composite substrate exhibited not only a wide linear range from 1.00 to 1.00 × 10<sup>3</sup> nmol/L but also a limit of detection (LOD) as low as 0.92 nmol/L for Methylene Blue (MB) molecules. In addition, we monitored DA release during the differentiation of neural stem cells (NSCs) into dopaminergic neurons by virtue of our constructed SERS platform in a nondestructive and in situ manner. Consequently, the developed SERS platform facilitates the direct detection of exocytotic DA from neuronal cells, enabling real-time assessment of cell viability in models of neurodegenerative disease-related cellular damage.</div></div>\",\"PeriodicalId\":433,\"journal\":{\"name\":\"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy\",\"volume\":\"332 \",\"pages\":\"Article 125845\"},\"PeriodicalIF\":4.6000,\"publicationDate\":\"2025-05-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1386142525001519\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/2/2 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q1\",\"JCRName\":\"SPECTROSCOPY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1386142525001519","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/2/2 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"SPECTROSCOPY","Score":null,"Total":0}
Surface-enhanced Raman scattering probes based on sea urchin-like Bi2S3@Co3O4 composite for the detection of dopamine during neural stem cell differentiation
Dopamine (DA) is a significant neurotransmitter involved in various functions of the central nervous system, containing the regulation of motor functions, arousal, motivation and reward processing. Therefore, precise measurement of DA concentrations is vital for studying different dopaminergic neural pathways and for the diagnosis of neurological disorders. Herein, we developed a new semiconductor surface-enhanced Raman spectroscopic (SERS) platform designed for the real-time monitoring DA release from live cells with high selectivity and sensitivity. The sea urchin-like Bi2S3@Co3O4 composite substrate exhibited not only a wide linear range from 1.00 to 1.00 × 103 nmol/L but also a limit of detection (LOD) as low as 0.92 nmol/L for Methylene Blue (MB) molecules. In addition, we monitored DA release during the differentiation of neural stem cells (NSCs) into dopaminergic neurons by virtue of our constructed SERS platform in a nondestructive and in situ manner. Consequently, the developed SERS platform facilitates the direct detection of exocytotic DA from neuronal cells, enabling real-time assessment of cell viability in models of neurodegenerative disease-related cellular damage.
期刊介绍：
Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science.
The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments.
Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate.
Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to:
Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences,
Novel experimental techniques or instrumentation for molecular spectroscopy,
Novel theoretical and computational methods,
Novel applications in photochemistry and photobiology,
Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.
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