{"title":"对helveticus Lactobacillus， Kluyveromyces marxianus在单培养和共同培养中用于生产螺旋藻发酵产品的优化","authors":"Naciye Ozturk , Cansu Yay , Zeynep Özlem Cinar , Hazal Nazlıcan Atalay , Onur Guneser , Muge Isleten Hosoglu , Tugba Boyunegmez Tumer","doi":"10.1016/j.algal.2025.103948","DOIUrl":null,"url":null,"abstract":"<div><div>Present study was carried out to optimize certain fermentation parameters when <em>L. helveticus</em> B-4526 and <em>K. marxianus</em> Y-329 were used in monoculture and co-culture in a medium supplemented with dried <em>Spirulina platensis</em> biomass (Spirulina medium). Maximum cell number increase for <em>L. helveticus</em>, <em>K. marxianus</em> in Spirulina medium was calculated as 2.40 and 3.80 log CFU mL<sup>−1</sup>, respectively. It was observed that proteins were rapidly hydrolyzed by <em>L. helveticus</em>. Consequently, the concentrations of some specific amino acids changed according to product type. The fermented Spirulina (FS) by <em>K. marxi</em>anus resulted in higher trimethyl pyrazine content (2636.66 μg kg<sup>−1</sup>) than its unFS counterpart (2088.08 μg kg<sup>−1</sup>). All FS products resulted in increased inhibitory activity on acetylcholinesterase enzyme. FS product by co-culture of both organisms demonstrated a distinct suppressive effect on LPS-induced inflammatory NO secretion in RAW264.7 macrophage cell line. Besides, FS products did not show cytotoxicity in endothelial and macrophage cell lines, therefore presenting more biocompatible nature as compared to their unFS counterparts.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"86 ","pages":"Article 103948"},"PeriodicalIF":4.5000,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Optimizations related to the use of Lactobacillus helveticus, Kluyveromyces marxianus in monoculture, and co-culture for production of Spirulina-based fermented products\",\"authors\":\"Naciye Ozturk , Cansu Yay , Zeynep Özlem Cinar , Hazal Nazlıcan Atalay , Onur Guneser , Muge Isleten Hosoglu , Tugba Boyunegmez Tumer\",\"doi\":\"10.1016/j.algal.2025.103948\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Present study was carried out to optimize certain fermentation parameters when <em>L. helveticus</em> B-4526 and <em>K. marxianus</em> Y-329 were used in monoculture and co-culture in a medium supplemented with dried <em>Spirulina platensis</em> biomass (Spirulina medium). Maximum cell number increase for <em>L. helveticus</em>, <em>K. marxianus</em> in Spirulina medium was calculated as 2.40 and 3.80 log CFU mL<sup>−1</sup>, respectively. It was observed that proteins were rapidly hydrolyzed by <em>L. helveticus</em>. Consequently, the concentrations of some specific amino acids changed according to product type. The fermented Spirulina (FS) by <em>K. marxi</em>anus resulted in higher trimethyl pyrazine content (2636.66 μg kg<sup>−1</sup>) than its unFS counterpart (2088.08 μg kg<sup>−1</sup>). All FS products resulted in increased inhibitory activity on acetylcholinesterase enzyme. FS product by co-culture of both organisms demonstrated a distinct suppressive effect on LPS-induced inflammatory NO secretion in RAW264.7 macrophage cell line. Besides, FS products did not show cytotoxicity in endothelial and macrophage cell lines, therefore presenting more biocompatible nature as compared to their unFS counterparts.</div></div>\",\"PeriodicalId\":7855,\"journal\":{\"name\":\"Algal Research-Biomass Biofuels and Bioproducts\",\"volume\":\"86 \",\"pages\":\"Article 103948\"},\"PeriodicalIF\":4.5000,\"publicationDate\":\"2025-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Algal Research-Biomass Biofuels and Bioproducts\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2211926425000578\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/2/4 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q1\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Algal Research-Biomass Biofuels and Bioproducts","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2211926425000578","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/2/4 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Optimizations related to the use of Lactobacillus helveticus, Kluyveromyces marxianus in monoculture, and co-culture for production of Spirulina-based fermented products
Present study was carried out to optimize certain fermentation parameters when L. helveticus B-4526 and K. marxianus Y-329 were used in monoculture and co-culture in a medium supplemented with dried Spirulina platensis biomass (Spirulina medium). Maximum cell number increase for L. helveticus, K. marxianus in Spirulina medium was calculated as 2.40 and 3.80 log CFU mL−1, respectively. It was observed that proteins were rapidly hydrolyzed by L. helveticus. Consequently, the concentrations of some specific amino acids changed according to product type. The fermented Spirulina (FS) by K. marxianus resulted in higher trimethyl pyrazine content (2636.66 μg kg−1) than its unFS counterpart (2088.08 μg kg−1). All FS products resulted in increased inhibitory activity on acetylcholinesterase enzyme. FS product by co-culture of both organisms demonstrated a distinct suppressive effect on LPS-induced inflammatory NO secretion in RAW264.7 macrophage cell line. Besides, FS products did not show cytotoxicity in endothelial and macrophage cell lines, therefore presenting more biocompatible nature as compared to their unFS counterparts.
期刊介绍：
Algal Research is an international phycology journal covering all areas of emerging technologies in algae biology, biomass production, cultivation, harvesting, extraction, bioproducts, biorefinery, engineering, and econometrics. Algae is defined to include cyanobacteria, microalgae, and protists and symbionts of interest in biotechnology. The journal publishes original research and reviews for the following scope: algal biology, including but not exclusive to: phylogeny, biodiversity, molecular traits, metabolic regulation, and genetic engineering, algal cultivation, e.g. phototrophic systems, heterotrophic systems, and mixotrophic systems, algal harvesting and extraction systems, biotechnology to convert algal biomass and components into biofuels and bioproducts, e.g., nutraceuticals, pharmaceuticals, animal feed, plastics, etc. algal products and their economic assessment
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