Hepatoprotective effect of Jasminum sambac decoction against paracetamol-induced toxicity: in silico, in vitro and in vivo studies.

Objective: We aimed to investigate the pharmacologic mechanism underlying this traditional remedy.

Method: We used a freshly produced hot water decoction of J. sambac leaves (HWDJS) to examine its hepatoprotective effects in laboratory animals, administering doses of HWDJS at 100, 200, and 300 mg/kg over 7 days. On the 8th day, paracetamol toxicity was induced at a dose of 2 g/kg. The test and control groups assessed biochemical indicators (alkaline phosphatase, aspartate transaminase, alanine transaminase, and C-reactive protein), liver-to-body weight ratio, and cytoarchitecture. Oxidative biomarkers (gamma-glutamyl-glycine, superoxide dismutases, and malondialdehyde) were measured after 24 hours of toxicity induction to judge the extent of hepatoprotection.

Result: HWDJS confirmed a significant (P ≤ 0.05) decrease in biochemical markers compared to the intoxicated group: histopathologic differences and antioxidant measures. The antioxidant potential of HWDJS was confirmed by a 2,2-diphenyl-1-picrylhydrazyl and nitric oxide scavenging assay.

Conclusion: The findings prove that the decoction of J. sambac has extensive hepatoprotection against paracetamol-induced toxicity and can be considered as an alternative therapy in hepatic disorders.