“修复和折叠”DNA纳米镊子用于测量ALKBH介导的DNA烷基化修复

IF 3.3 3区化学 Q2 CHEMISTRY, ANALYTICAL Analyst Pub Date : 2025-02-24 DOI:10.1039/D5AN00099H

Fengze Jiang, Cui Zhang, Xiangnan Wang, Yuan Yin, Jieling Hong, Hao Tang, Li-Juan Tang and Zhenkun Wu

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引用次数: 0

摘要

我们报道了一种新的DNA损伤修饰纳米镊，它在ALKBH酶介导的损伤修复中经历了构象开关。我们证明了DNA损伤修饰的纳米镊子能够特异性和快速地监测ALKBH2和ALKBH3的活性并筛选抑制剂。重要的是，该策略允许评估细胞中alkbh2相关的耐药性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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“Repair and fold” DNA nanotweezers for measuring DNA alkylation repair mediated by ALKBH†

We report a novel DNA lesion-modified nanotweezer that underwent conformational switching upon ALKBH enzyme-mediated lesion repair. We demonstrated that the DNA lesion-modified nanotweezer enabled specific and rapid monitoring of ALKBH2 and ALKBH3 activities and screening of inhibitors. Importantly, this strategy allowed evaluating ALKBH2-associated drug resistance in cells.

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