首例使用MALDI-TOF质谱法直接从全血中鉴定血液感染儿童肿瘤血液病患者的微生物。

IF 5.5 2区医学 Q1 INFECTIOUS DISEASES Antibiotics-Basel Pub Date : 2025-02-04 DOI:10.3390/antibiotics14020149

Venere Cortazzo, Maria Del Carmen Pereyra Boza, Vanessa Tuccio Guarna Assanti, Gianluca Foglietta, Gianluca Vrenna, Marilena Agosta, Elena Chaiter, Martina Rossitto, Barbara Lucignano, Manuela Onori, Valeria Fox, Marco Becilli, Pietro Merli, Filippo Frioni, Carlo Federico Perno, Paola Bernaschi

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引用次数: 0

摘要

背景：血液感染影响了多达20%接受重症监护的儿科癌症患者，显著增加了发病率和死亡率，据报道，与感染相关的死亡率高达16%。方法：由于宿主基因组物质的干扰、低细菌负荷、全血的内源性成分和外源性物质的干扰等因素，直接从全血中鉴定微生物是困难的。然而，快速的微生物诊断对这些患者仍然至关重要。结果和结论：在这里，我们报告了第一例使用MALDI-TOF质谱法直接从全血中鉴定细菌病原体的病例，该病例是意大利罗马Bambino Gesù儿童医院（IRCCS）收治的一名患有败血症的肿瘤血液学儿童患者。

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The First Case of the Identification of a Microorganism Directly from Whole Blood Using MALDI-TOF Mass Spectrometry in an Onco-Hematological Pediatric Patient with Bloodstream Infection.

Background: Bloodstream infections affect up to 20% of pediatric cancer patients receiving intensive care, contributing significantly to morbidity and mortality, with infection-related mortality rates reported to be as high as 16%.

Methods: The identification of microorganisms directly from whole blood is difficult due to several factors, such as interference from host genomic material, low bacterial load, the endogenous components of whole blood and exogenous substances, which can interfere with the identification process. Nevertheless, rapid microbial diagnosis remains of paramount importance in these patients.

Results and conclusion: Here, we present the first case of bacterial pathogen identification directly from whole blood using MALDI-TOF mass spectrometry in an onco-hematological pediatric patient affected by sepsis and admitted to Bambino Gesù Children's Hospital (IRCCS) in Rome, Italy.

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来源期刊

Antibiotics-Basel Pharmacology, Toxicology and Pharmaceutics-General Pharmacology, Toxicology and Pharmaceutics

CiteScore

7.30

自引率

14.60%

发文量

1547

审稿时长

11 weeks

期刊介绍： Antibiotics (ISSN 2079-6382) is an open access, peer reviewed journal on all aspects of antibiotics. Antibiotics is a multi-disciplinary journal encompassing the general fields of biochemistry, chemistry, genetics, microbiology and pharmacology. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on the length of papers.