Ran Wu , Ya-Fei Song , Yanlong Gu, Jiang Li, Zhipeng Niu, Dong-Hui Zhou
{"title":"基于GRA6和SAG1的猪刚地弓形虫抗体化学发光免疫检测","authors":"Ran Wu , Ya-Fei Song , Yanlong Gu, Jiang Li, Zhipeng Niu, Dong-Hui Zhou","doi":"10.1016/j.vetpar.2025.110437","DOIUrl":null,"url":null,"abstract":"<div><div>Toxoplasmosis is a zoonotic protozoan disease caused by <em>Toxoplasma gondii</em>. Swine serve as intermediate hosts in the transmission of <em>T. gondii</em>, rendering the detection of this pathogen in swine populations is essential for public health. The chemiluminescence immunoassay (CLIA) is a highly regarded quantitative serological technique recognized for its exceptional sensitivity, specificity, and extensive linear range, thereby facilitating its application in the detection of swine diseases. In this study, we developed CLIA assays using GRA6 (dense granule protein 6), SAG1 (surface antigens 1), and GRA6-SAG1 antigens to detect <em>T. gondii</em> in pigs, achieving results in just 20 minutes. Testing 148 serum samples revealed that the GRA6-SAG1 CLIA had the highest diagnostic performance, with a sensitivity of 97.96 %, specificity of 100.00 %, and an intra-batch coefficient of variation below 8.00 %. Furthermore, analysis of 300 serum samples collected from swine in Fujian province showed the positive rates the <em>T. gondii</em> detection by the GRA6, SAG1, and GRA6-SAG1 CLIA assays is 66.00 %, 68.67 %, and 74.67 %, respectively. The <em>T. gondiii</em> positive rates were higher than those of commercial ELISA kits A (61.00 %) and B (16.33 %) as well as indirect hemagglutination (IHA) kit C (8.33 %). The GRA6-SAG1 CLIA developed in this study presents a rapid, specific, sensitive, and stable method for the detection of antibodies against <em>T. gondii</em>, holding significant potential for the accurate diagnosis of toxoplasmosis in swine.</div></div>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"335 ","pages":"Article 110437"},"PeriodicalIF":2.7000,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A chemiluminescence immunoassay for detection of Toxoplasma gondii antibody in porcine based on GRA6 and SAG1\",\"authors\":\"Ran Wu , Ya-Fei Song , Yanlong Gu, Jiang Li, Zhipeng Niu, Dong-Hui Zhou\",\"doi\":\"10.1016/j.vetpar.2025.110437\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Toxoplasmosis is a zoonotic protozoan disease caused by <em>Toxoplasma gondii</em>. Swine serve as intermediate hosts in the transmission of <em>T. gondii</em>, rendering the detection of this pathogen in swine populations is essential for public health. The chemiluminescence immunoassay (CLIA) is a highly regarded quantitative serological technique recognized for its exceptional sensitivity, specificity, and extensive linear range, thereby facilitating its application in the detection of swine diseases. In this study, we developed CLIA assays using GRA6 (dense granule protein 6), SAG1 (surface antigens 1), and GRA6-SAG1 antigens to detect <em>T. gondii</em> in pigs, achieving results in just 20 minutes. Testing 148 serum samples revealed that the GRA6-SAG1 CLIA had the highest diagnostic performance, with a sensitivity of 97.96 %, specificity of 100.00 %, and an intra-batch coefficient of variation below 8.00 %. Furthermore, analysis of 300 serum samples collected from swine in Fujian province showed the positive rates the <em>T. gondii</em> detection by the GRA6, SAG1, and GRA6-SAG1 CLIA assays is 66.00 %, 68.67 %, and 74.67 %, respectively. The <em>T. gondiii</em> positive rates were higher than those of commercial ELISA kits A (61.00 %) and B (16.33 %) as well as indirect hemagglutination (IHA) kit C (8.33 %). The GRA6-SAG1 CLIA developed in this study presents a rapid, specific, sensitive, and stable method for the detection of antibodies against <em>T. gondii</em>, holding significant potential for the accurate diagnosis of toxoplasmosis in swine.</div></div>\",\"PeriodicalId\":23716,\"journal\":{\"name\":\"Veterinary parasitology\",\"volume\":\"335 \",\"pages\":\"Article 110437\"},\"PeriodicalIF\":2.7000,\"publicationDate\":\"2025-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Veterinary parasitology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0304401725000482\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/2/27 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"PARASITOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary parasitology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0304401725000482","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/2/27 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"PARASITOLOGY","Score":null,"Total":0}
A chemiluminescence immunoassay for detection of Toxoplasma gondii antibody in porcine based on GRA6 and SAG1
Toxoplasmosis is a zoonotic protozoan disease caused by Toxoplasma gondii. Swine serve as intermediate hosts in the transmission of T. gondii, rendering the detection of this pathogen in swine populations is essential for public health. The chemiluminescence immunoassay (CLIA) is a highly regarded quantitative serological technique recognized for its exceptional sensitivity, specificity, and extensive linear range, thereby facilitating its application in the detection of swine diseases. In this study, we developed CLIA assays using GRA6 (dense granule protein 6), SAG1 (surface antigens 1), and GRA6-SAG1 antigens to detect T. gondii in pigs, achieving results in just 20 minutes. Testing 148 serum samples revealed that the GRA6-SAG1 CLIA had the highest diagnostic performance, with a sensitivity of 97.96 %, specificity of 100.00 %, and an intra-batch coefficient of variation below 8.00 %. Furthermore, analysis of 300 serum samples collected from swine in Fujian province showed the positive rates the T. gondii detection by the GRA6, SAG1, and GRA6-SAG1 CLIA assays is 66.00 %, 68.67 %, and 74.67 %, respectively. The T. gondiii positive rates were higher than those of commercial ELISA kits A (61.00 %) and B (16.33 %) as well as indirect hemagglutination (IHA) kit C (8.33 %). The GRA6-SAG1 CLIA developed in this study presents a rapid, specific, sensitive, and stable method for the detection of antibodies against T. gondii, holding significant potential for the accurate diagnosis of toxoplasmosis in swine.
期刊介绍：
The journal Veterinary Parasitology has an open access mirror journal,Veterinary Parasitology: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review.
This journal is concerned with those aspects of helminthology, protozoology and entomology which are of interest to animal health investigators, veterinary practitioners and others with a special interest in parasitology. Papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites in all domesticated animals, fall within the scope of the journal. Papers of geographically limited (local) interest which are not of interest to an international audience will not be accepted. Authors who submit papers based on local data will need to indicate why their paper is relevant to a broader readership.
Parasitological studies on laboratory animals fall within the scope of the journal only if they provide a reasonably close model of a disease of domestic animals. Additionally the journal will consider papers relating to wildlife species where they may act as disease reservoirs to domestic animals, or as a zoonotic reservoir. Case studies considered to be unique or of specific interest to the journal, will also be considered on occasions at the Editors'' discretion. Papers dealing exclusively with the taxonomy of parasites do not fall within the scope of the journal.
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