Tricarbonylrhenium(I) complexes with aggregation-induced phosphorescence emission (AIPE) properties: Application to the selective detection of heparin and its main contaminant

Phosphorescent tricarbonylrhenium(I) complexes that are more emissive in the aggregate state than in solution could be very valuable probes for biological analyses, but their development is delicate. The present work focuses on the synthesis and spectroscopic study of three new complexes that differ by the nature of their positively charged substituent. The dissolved complexes were very weakly emissive. Due to electrostatic interaction and aggregation, they showed a strong aggregation-induced phosphorescence enhancement (AIPE) effect in the presence of heparin, a polyanionic macromolecule of biological interest, and a weaker effect in the presence of chondroitin sulfate, which contains fewer negative charges. The magnitude of the AIPE effect depended on the nature and number of the cationic groups borne by the complex. However, it was weaker than expected from the behavior of the parent neutral complex studied in a conventional acetonitrile/water system, which highlights the challenge of accurately predicting solid-state emission properties for this class of molecules. This work introduces rhenium(I) complexes in the field of AIPE-active probes for the detection of polyanionic biomolecules.