Combination with isopentenyl diphosphate isomerase gene affects expression of two linalool/nerolidol synthases isoforms from Lingzhi

GlSTS21 and GsSTS41, derived from Ganoderma lucidum (Leyss. ex Fr.) Karst. and G. sinense Zhao, Xu et Zhang, respectively, have been identified as linalool/nerolidol synthases. Although both enzymes catalyze the synthesis of linalool and nerolidol, they exhibit distinct sequences and conserved structural domains, as well as variations in their secondary and tertiary structures, and differences in the location and number of substrate binding sites. When subjected to identical modification methods, GlSTS21 and GsSTS41 demonstrated divergent production trends. Specifically, GlSTS21 achieved the highest production of nerolidol when constructed in the sequence of pET28a-T7-GlSTS21-T7-E. coli isopentenyl diphosphate isomerase (IDI). Conversely, the highest production of linalool by GlSTS21 occurred when it was arranged in the sequence of pET28a-T7-GlSTS21-T7-E. coli IDI. For GsSTS41, the optimal production of both linalool and nerolidol was attained when it was ligated in the sequence of pET28a-T7-E. coli IDI-T7 −GsSTS41. These findings provide valuable insights for future efforts aimed at optimizing product-focused selection in industrial production processes.