接力投影显微望远镜

IF 23.4 Q1 OPTICS Light-Science & Applications Pub Date : 2025-03-07 DOI:10.1038/s41377-025-01800-6
Wenjun Yi, Shuyue Zhu, Meicheng Fu, Nan Gu, Junli Qi, Siyu Liu, Mengjun Zhu, Ping Wang, Xin Chen, Yi Zhang, Hongyu Zhang, Yao Xu, Junyi Du, Peng Xiong, Zhaohua Dong, Luobing Dong, Qiong Liu, Xiujian Li
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引用次数: 0

摘要

空间分辨率和成像距离之间的基本权衡对当前的成像技术,例如用于现代生物医学诊断和遥感的成像技术提出了重大挑战。本文介绍了一种用于成像动态幅相混合物体的新概念方法,称为中继投影显微望远镜(rPMT),它从根本上挑战了传统的光收集技术,通过平方律中继投影机制采用非视线光收集。我们成功地从中继屏幕上捕获的单次空间功率谱图像中分辨出距离分别为1019.0 mm、26.4 m和96.0 m的物体的2.76 μm、22.10 μm和35.08 μm的微小特征;结果表明,在不同距离上,rPMT的分辨能力明显超过了25 mm孔径相机镜头的阿贝衍射极限,分别达到了7.9、25.4和58.2的分辨提升系数。rPMT具有长距离、宽范围、高分辨率的成像能力,即使在物体被散射介质遮挡的情况下,其成像能力也超过了相机镜头的衍射极限和对焦范围极限。rPMT可以使用简单的设备,包括激光二极管、便携式相机和漫反射白板,实现从厘米到数百米的微米级分辨率的望远镜成像。与当代高分辨率成像技术不同,我们的方法不需要标记试剂、波前调制、合成接收孔径或平面扫描,这大大降低了成像系统的复杂性,提高了应用的实用性。这种方法对体内无标签动态生物医学显微成像诊断和小物体的远程监测具有特殊的前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Relay-projection microscopic telescopy

The fundamental trade-off between spatial resolution and imaging distance poses a significant challenge for current imaging techniques, such as those used in modern biomedical diagnosis and remote sensing. Here, we introduce a new conceptual method for imaging dynamic amplitude-phase-mixed objects, termed relay-projection microscopic telescopy (rPMT), which fundamentally challenges conventional light collection techniques by employing non-line-of-sight light collection through square-law relay-projection mechanisms. We successfully resolved tiny features measuring 2.76 μm, 22.10 μm, and 35.08 μm for objects positioned at distances of 1019.0 mm, 26.4 m, and 96.0 m, respectively, from single-shot spatial power spectrum images captured on the relay screen; these results demonstrate that the resolution capabilities of rPMT significantly surpass the Abbe diffraction limit of the 25 mm-aperture camera lens at the respective distances, achieving resolution improvement factors of 7.9, 25.4, and 58.2. The rPMT exhibits long-distance, wide-range, high-resolution imaging capabilities that exceed the diffraction limit of the camera lens and the focusing range limit, even when the objects are obscured by a scattering medium. The rPMT enables telescopic imaging from centimeters to beyond hundreds of meters with micrometer-scale resolution using simple devices, including a laser diode, a portable camera, and a diffusely reflecting whiteboard. Unlike contemporary high-resolution imaging techniques, our method does not require labeling reagents, wavefront modulation, synthetic receive aperture, or ptychography scanning, which significantly reduce the complexity of the imaging system and enhance the application practicality. This method holds particular promise for in-vivo label-free dynamic biomedical microscopic imaging diagnosis and remote surveillance of small objects.

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来源期刊
Light-Science & Applications
Light-Science & Applications 数理科学, 物理学I, 光学, 凝聚态物性 II :电子结构、电学、磁学和光学性质, 无机非金属材料, 无机非金属类光电信息与功能材料, 工程与材料, 信息科学, 光学和光电子学, 光学和光电子材料, 非线性光学与量子光学
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803
审稿时长
2.1 months
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