IF 1.1 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Nucleosides, Nucleotides & Nucleic Acids Pub Date : 2025-03-07 DOI:10.1080/15257770.2025.2466427
Jiayue Ding, Chun Xiang
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引用次数: 0

摘要

目的:利用DNA芯片技术,比较人类增生性疤痕(HTS)与正常皮肤组织mRNA表达谱的差异。通过生物信息学分析差异基因,从分子水平探讨 HTS 的发病机制,为 HTS 的临床治疗提供新的靶点:方法:收集三份 HTS 样本及其邻近的正常皮肤样本。方法:采集三个 HTS 样本及其邻近的正常皮肤样本,提取总 RNA 进行 cDNA 芯片分析。利用 Genespring 10.0 软件筛选差异表达基因,并对组内 HTS 和正常皮肤组进行聚类分析,利用 DAVID 生物信息学资源 6.7 对差异表达基因进行基因本体(GO)和生物通路分析:在 3 个 HTS 样品中,有 3832 条 mRNA 变化超过 2 倍,1920 条 mRNA 上调超过 2 倍,1912 条 mRNA 下调超过 2 倍,18 条 mRNA 上调超过 5 倍,29 条 mRNA 下调超过 5 倍。GO分析结果显示,CDKN1C、CDKN2A、CTNNA3、COL6A3、HOXB4等差异表达基因与细胞周期、细胞增殖、细胞粘附等生物学过程密切相关。kegg通路富集分析表明,TGF-β1、CDKN1C、CDKN2A、CDC14A、ITGB6、EGF等差异表达基因主要参与粘附斑块的形成、β转化因子信号通路、细胞周期信号通路、P53信号通路以及肿瘤相关信号通路:人类 HTS 样本的 mRNA 表达谱与正常皮肤样本相比发生了显著变化。TGF-β1、SMAD2、SMAD7、BAX、IGF2、COL1A1、COL1A2、MMPs、CDC14A、ITGB6、EGF、CDKN1C、CDKN2A、CTNNA3、HOXA3及其他参与生物过程、分子功能、信号通路的相关基因可能与增生性疤痕的发生和发展密切相关。
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Bioinformatics analysis of differentially expressed genes in hyperplastic scars using microarray data.

Objective: Using DNA microarray technology, we compared the differences in mRNA expression profiles between human hypertrophic scars (HTS) and normal skin tissues. Analyzing the differential genes in bioinformatics, to explore the pathogenesis of HTS at the molecular level, and to provide new targets for clinical treatment of HTS.

Methods: Three HTS samples and their adjacent normal skin samples were collected. The extraction of total RNA was performed for cDNA microarray analysis. The screening of differentially expressed genes was carried out by using Genespring 10.0 software, and cluster analysis was performed between HTS and normal skin groups within the group, and Gene Ontology (GO) and biological pathway analysis were performed for differentially expressed genes by using DAVID Bioinformatics Resources 6.7.

Results: In the 3 HTS samples, 3832 mRNAs overlapped in 3 HTS samples with more than 2-fold changes, 1920 mRNAs with more than 2-fold up-regulation, 1912 mRNAs with more than 2-fold down-regulation, 18 mRNAs with more than 5-fold up-regulation, and 29 mRNAs with more than 5-fold down-regulation. The results of the GO analysis showed that CDKN1C, CDKN2A, CTNNA3, COL6A3, HOXB4 and other differentially expressed genes are closely related to biological processes such as cell cycle, cell proliferation, and cell adhesion. The kegg pathway enrichment analysis showed that TGF-β1, CDKN1C, CDKN2A, CDC14A, ITGB6, EGF and other differentially expressed genes are mainly involved in the formation of adhesion plaques, β transforming factor signaling pathways, cell cycle signaling pathways, P53 signaling pathways, and tumor-related signaling pathways.

Conclusion: The mRNA expression profile of human HTS samples showed significant changes compared to normal skin samples. TGF-β1, SMAD2, SMAD7, BAX, IGF2, COL1A1, COL1A2, MMPs, CDC14A, ITGB6, EGF, CDKN1C, CDKN2A, CTNNA3, HOXA3 and other related genes involved in biological processes, molecular functions, signaling pathways may be closely related to the occurrence and development of hypertrophic scars.

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来源期刊
Nucleosides, Nucleotides & Nucleic Acids
Nucleosides, Nucleotides & Nucleic Acids 生物-生化与分子生物学
CiteScore
2.60
自引率
7.70%
发文量
91
审稿时长
6 months
期刊介绍: Nucleosides, Nucleotides & Nucleic Acids publishes research articles, short notices, and concise, critical reviews of related topics that focus on the chemistry and biology of nucleosides, nucleotides, and nucleic acids. Complete with experimental details, this all-inclusive journal emphasizes the synthesis, biological activities, new and improved synthetic methods, and significant observations related to new compounds.
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