Heatwave enhance the adaptability of Chlorella pyrenoidosa to zinc oxide nanoparticles: Regulation of interfacial interactions and metabolic mechanisms

Wide application of zinc oxide nanoparticles (ZnO NPs) and increasing frequency of heatwaves (HWs) have posed a great threat to freshwater ecosystems, while phytotoxicity of ZnO NPs mediated by HWs remains unclear. This study aims to link the physiological responses, bio-nano interactions, and metabolic mechanisms of Chlorella pyrenoidosa with ZnO NPs under heat stress. Results demonstrated a temperature-dependent growth inhibition against ZnO NPs, with a higher reduction of growth rate at 24 °C than 28 °C. Accompanied with lower reactive oxidative stress and cell damage at 28 °C, our results indicated that HW could enhance the adaptability of C. pyrenoidosa to ZnO NPs stress. Furthermore, HW induced the variation of algal surface properties, altered interfacial interactions in the bio-nano system, and decreased cellular Zn uptake. Metabolomics analysis supported the temperature-dependent influences of ZnO NPs on C. pyrenoidosa. The phytotoxicity of ZnO NPs was associated with the disturbance of amino acids, fatty acids, and energy metabolic processes, which were mitigated under HW condition, enhancing the responsiveness of algae to the adverse effects. These results emphasize the importance of taking the impacts of HWs into account when evaluating the environmental risks of ZnO NPs.