Dongming Wang, Zhitao Geng, Jiming Shen, Tingting Xie, Fan Wu, Yu Zhang, Yiwei Dong, Dongmei Mao, Yanling Ji, Yan Huang, Zhoukun Li, Yongheng Liang, Xianfeng Ye, Zhongli Cui
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引用次数: 0
摘要
草莓炭疽病是由草莓炭疽菌（Colletotrichum gloeosporioides）引起的一种真菌病，可给草莓采后造成重大经济损失。生物防治是一种更环保、更安全的策略。本研究发现，太阳伯克霍尔德菌（Burkholderia sola NAU20）产生的挥发性化合物（VOCs）显著抑制了gloeosporioides的菌丝生长和分生孢子萌发。反式-2-辛酸乙酯的抑菌活性最强，最低抑菌浓度（MIC）为0.448 mL L−1。反式-2-辛酸乙酯处理后，真菌细胞壁结构被严重破坏，ROS的大量积累和DNA的断裂表明细胞可能被诱导凋亡。反式-2-辛酸乙酯能在5 d内以2 ×MIC的水平完全抑制草莓对gloeosporioides的侵染。此外，反式-2-辛酸乙酯除具有直接的抗真菌作用外，还能诱导苯丙素和黄酮类生物合成增强对真菌侵染的抑制作用。
Biocontrol effects of volatile organic compounds released from Burkholderia sola NAU20 to strawberry anthracnose caused by Colletotrichum gloeosporioides
Strawberry anthracnose is a fungal disease caused by Colletotrichum gloeosporioides, which can lead to significant economic losses in postharvest strawberries. Biological control is a greener and safer strategy. In this study, the hyphal growth and conidial germination of Colletotrichum gloeosporioides were found to be significantly inhibited by volatile compounds (VOCs) produced by Burkholderia sola NAU20. Ethyl trans-2-octenoate demonstrated the strongest antifungal activity among the tested VOCs, with a minimum inhibitory concentration (MIC) of 0.448 mL L−1. The structure of fungal cell wall was severely disrupted, and a significant accumulation of ROS and DNA fragmentation indicated that apoptosis may be induced in ethyl trans-2-octenoate treated cells. Ethyl trans-2-octenoate can completely inhibit the infection of C. gloeosporioides on strawberries at a level of 2 ×MIC within 5 d. Furthermore, in addition to the direct antifungal effect of ethyl trans-2-octenoate, the induction of phenylpropanoids and flavonoids biosynthesis enhanced the suppression of fungal infection.
期刊介绍：
The journal is devoted exclusively to the publication of original papers, review articles and frontiers articles on biological and technological postharvest research. This includes the areas of postharvest storage, treatments and underpinning mechanisms, quality evaluation, packaging, handling and distribution of fresh horticultural crops including fruit, vegetables, flowers and nuts, but excluding grains, seeds and forages.
Papers reporting novel insights from fundamental and interdisciplinary research will be particularly encouraged. These disciplines include systems biology, bioinformatics, entomology, plant physiology, plant pathology, (bio)chemistry, engineering, modelling, and technologies for nondestructive testing.
Manuscripts on fresh food crops that will be further processed after postharvest storage, or on food processes beyond refrigeration, packaging and minimal processing will not be considered.
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