{"title":"鼠尾草籽油抗氧化和酶抑制作用：LC-HR/MS综合植物化学筛选","authors":"Muzaffer Mutlu , Zeynebe Bingol , Eda Mehtap Ozden , Ekrem Köksal , Adem Erturk , Ahmet C. Goren , Saleh Alwasel , İlhami Gulcin","doi":"10.1016/j.ejbt.2024.12.002","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><div>In this study, the antioxidant and anti-Alzheimer's disease properties of Chia (<em>Salvia hispanica</em>) seed oil (CSO) were determined for the first time. Three different metal reduction and two different radical scavenging methods were used to determine the antioxidant properties of CSO. It exhibited higher antioxidant activity than vitamins E and C in the CUPRAC method.</div></div><div><h3>Results</h3><div>CSO has shown excellent efficacy in the antioxidant methods used in this study. In the DPPH radical scavenging method, CSO exhibited higher radical scavenging potential than BHT, a standard and synthetic antioxidant. The anti-Alzheimer's disease properties of CSO were determined by inhibition of acetylcholinesterase enzyme and its IC<sub>50</sub> value (17.60 µg/mL) was found to be close to the IC<sub>50</sub> value of tacrine (8.82 µg/mL), the standard inhibitor of the enzyme. Inhibition properties of α-glycosidase enzyme and human carbonic anhydrase II isoenzymes were also studied. It was understood that CSO inhibited both enzymes at a lower rate than standard inhibitors. Also, the total phenolic and flavonoid contents of CSO were determined as 784.44 µg gallic acid equivalent (GAE)/mL oil and quercetin 150.00 µg QE)/mL oil, respectively. In addition, LC-HRMS chromatography application was performed to understand the phenolic content of CSO. It was determined that isosakuranetin (29.07 mg/L oil) was the most abundant polyphenolic compound in CSA. Also, seven polyphenolics of the studied remained below the detectable amount.</div></div><div><h3>Conclusions</h3><div>It was found that CSO had effective antioxidant activity, polyphenolic contents and potent enzyme inhibition properties, associated with some global disease.</div><div><strong>How to cite:</strong> Mutlu M, Bingol Z, Ozden EM, et al. Antioxidant, and enzyme inhibition effects of chia (<em>Salvia hispanica</em>) seed oil: A comprehensive phytochemical screening using LC-HR/MS. Electron J Biotechnol 2025;74. <span><span>https://doi.org/10.1016/j.ejbt.2024.12.002</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"74 ","pages":"Pages 41-53"},"PeriodicalIF":2.5000,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Antioxidant, and enzyme inhibition effects of chia (Salvia hispanica) seed oil: A comprehensive phytochemical screening using LC-HR/MS\",\"authors\":\"Muzaffer Mutlu , Zeynebe Bingol , Eda Mehtap Ozden , Ekrem Köksal , Adem Erturk , Ahmet C. Goren , Saleh Alwasel , İlhami Gulcin\",\"doi\":\"10.1016/j.ejbt.2024.12.002\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><div>In this study, the antioxidant and anti-Alzheimer's disease properties of Chia (<em>Salvia hispanica</em>) seed oil (CSO) were determined for the first time. Three different metal reduction and two different radical scavenging methods were used to determine the antioxidant properties of CSO. It exhibited higher antioxidant activity than vitamins E and C in the CUPRAC method.</div></div><div><h3>Results</h3><div>CSO has shown excellent efficacy in the antioxidant methods used in this study. In the DPPH radical scavenging method, CSO exhibited higher radical scavenging potential than BHT, a standard and synthetic antioxidant. The anti-Alzheimer's disease properties of CSO were determined by inhibition of acetylcholinesterase enzyme and its IC<sub>50</sub> value (17.60 µg/mL) was found to be close to the IC<sub>50</sub> value of tacrine (8.82 µg/mL), the standard inhibitor of the enzyme. Inhibition properties of α-glycosidase enzyme and human carbonic anhydrase II isoenzymes were also studied. It was understood that CSO inhibited both enzymes at a lower rate than standard inhibitors. Also, the total phenolic and flavonoid contents of CSO were determined as 784.44 µg gallic acid equivalent (GAE)/mL oil and quercetin 150.00 µg QE)/mL oil, respectively. In addition, LC-HRMS chromatography application was performed to understand the phenolic content of CSO. It was determined that isosakuranetin (29.07 mg/L oil) was the most abundant polyphenolic compound in CSA. Also, seven polyphenolics of the studied remained below the detectable amount.</div></div><div><h3>Conclusions</h3><div>It was found that CSO had effective antioxidant activity, polyphenolic contents and potent enzyme inhibition properties, associated with some global disease.</div><div><strong>How to cite:</strong> Mutlu M, Bingol Z, Ozden EM, et al. Antioxidant, and enzyme inhibition effects of chia (<em>Salvia hispanica</em>) seed oil: A comprehensive phytochemical screening using LC-HR/MS. Electron J Biotechnol 2025;74. <span><span>https://doi.org/10.1016/j.ejbt.2024.12.002</span><svg><path></path></svg></span>.</div></div>\",\"PeriodicalId\":11529,\"journal\":{\"name\":\"Electronic Journal of Biotechnology\",\"volume\":\"74 \",\"pages\":\"Pages 41-53\"},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2025-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Electronic Journal of Biotechnology\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S071734582500003X\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Electronic Journal of Biotechnology","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S071734582500003X","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Antioxidant, and enzyme inhibition effects of chia (Salvia hispanica) seed oil: A comprehensive phytochemical screening using LC-HR/MS
Background
In this study, the antioxidant and anti-Alzheimer's disease properties of Chia (Salvia hispanica) seed oil (CSO) were determined for the first time. Three different metal reduction and two different radical scavenging methods were used to determine the antioxidant properties of CSO. It exhibited higher antioxidant activity than vitamins E and C in the CUPRAC method.
Results
CSO has shown excellent efficacy in the antioxidant methods used in this study. In the DPPH radical scavenging method, CSO exhibited higher radical scavenging potential than BHT, a standard and synthetic antioxidant. The anti-Alzheimer's disease properties of CSO were determined by inhibition of acetylcholinesterase enzyme and its IC50 value (17.60 µg/mL) was found to be close to the IC50 value of tacrine (8.82 µg/mL), the standard inhibitor of the enzyme. Inhibition properties of α-glycosidase enzyme and human carbonic anhydrase II isoenzymes were also studied. It was understood that CSO inhibited both enzymes at a lower rate than standard inhibitors. Also, the total phenolic and flavonoid contents of CSO were determined as 784.44 µg gallic acid equivalent (GAE)/mL oil and quercetin 150.00 µg QE)/mL oil, respectively. In addition, LC-HRMS chromatography application was performed to understand the phenolic content of CSO. It was determined that isosakuranetin (29.07 mg/L oil) was the most abundant polyphenolic compound in CSA. Also, seven polyphenolics of the studied remained below the detectable amount.
Conclusions
It was found that CSO had effective antioxidant activity, polyphenolic contents and potent enzyme inhibition properties, associated with some global disease.
How to cite: Mutlu M, Bingol Z, Ozden EM, et al. Antioxidant, and enzyme inhibition effects of chia (Salvia hispanica) seed oil: A comprehensive phytochemical screening using LC-HR/MS. Electron J Biotechnol 2025;74. https://doi.org/10.1016/j.ejbt.2024.12.002.
期刊介绍：
Electronic Journal of Biotechnology is an international scientific electronic journal, which publishes papers from all areas related to Biotechnology. It covers from molecular biology and the chemistry of biological processes to aquatic and earth environmental aspects, computational applications, policy and ethical issues directly related to Biotechnology.
The journal provides an effective way to publish research and review articles and short communications, video material, animation sequences and 3D are also accepted to support and enhance articles. The articles will be examined by a scientific committee and anonymous evaluators and published every two months in HTML and PDF formats (January 15th , March 15th, May 15th, July 15th, September 15th, November 15th).
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