Aptamer-based biosensors for wastewater surveillance of influenza virus, SARS-CoV-2, and norovirus: A comprehensive review

Wastewater-based epidemiological (WBE) surveillance has emerged as a crucial tool for monitoring infectious diseases within communities. However, its broader application is frequently constrained by the high costs, labor-intensive processes, and extended timeframes required for sample collection, transportation, and processing. Aptamer-based biosensors offer a promising alternative, leveraging the specific binding properties of aptamers to biomolecules for the on-site and rapid quantification of disease biomarkers in wastewater. This review systematically evaluates recent advancements in the application of aptamer-based biosensors for the detection of key pathogens, including influenza viruses, SARS-CoV-2, and norovirus, within wastewater matrices. The discussion encompasses the technical stability and reliability of signal transmission associated with these biosensors, as well as the current challenges faced in real-world implementation. Noteworthy progress has been made in the development of these biosensors for WBE, achieving detection limits as low as femtomolar (fM) levels in buffer and linear dynamic ranges extending up to five orders of magnitude for viruses such as influenza and SARS-CoV-2. Despite this progress, considerable hurdles remain to be addressed before these technologies can be effectively deployed in practical settings, especially within complex wastewater environments. Key factors affecting detection performance include matrix interference, environmental variability, and the diminished stability of both viral targets and aptamer-target interactions in wastewater. This review not only highlights these challenges but also outlines potential avenues for future research aimed at enhancing the functionality and applicability of aptamer-based biosensors in WBE, ultimately contributing to more effective public health surveillance and disease monitoring strategies.