用于冰控制的聚（l-蛋氨酸）的侧功能化。

IF 5.4 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Biomacromolecules Pub Date : 2025-04-14 Epub Date: 2025-03-14 DOI:10.1021/acs.biomac.5c00086

Qingjing Niu , Ke Shang , Huimin Han , Binlin Chen , Kongying Zhu , Lixia Ren , Xiaoyan Yuan

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引用次数: 0

摘要

在细胞冷冻保存过程中，控制冰的生长是至关重要的，但目前小分子作为冷冻保护剂的应用仍然是一个挑战。受天然抗冻（糖）蛋白结构的启发，本研究合成了具有不同侧基的功能化聚（l-蛋氨酸）s (PMets)，包括羟基、甲基和羟基的拟苏氨酸（PMet-MOH）、羧基和磺酸的两性离子（PMet-COOH）、甘油和海藻糖垂链。结果表明，这些功能化的pmet倾向于在水中自组装成100-300 nm带正电荷的纳米颗粒。功能结构对其控冰性能有显著影响。推测PMet- moh可能通过相邻甲基和羟基的吸附机制抑制冰的生长，而海藻糖系结的PMet可以限制水分子的扩散，抑制冰的再结晶活性最强，两性离子PMet- cooh明显促进冰的成核。这项工作为开发功能性多肽作为有前途的生物相容性冷冻保护剂提供了有价值的见解。

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Side-Functionalization of Poly(l‑methionine) for Ice Control

Controlling ice growth is crucial during the cryopreservation of cells, but the current application of small molecules as cryoprotectants still remains a challenge. Inspired by structures of natural antifreeze (glyco)proteins, in this work, functionalized poly(l-methionine)s (PMets) are synthesized with different side groups including hydroxyl, threonine-mimetic with both methyl and hydroxyl groups (PMet-MOH), zwitterion with carboxyl and sulfonium (PMet-COOH), glycerol, and trehalose pendants. Results suggest that these functionalized PMets tend to self-assemble into 100–300 nm nanoparticles with positive charges in water. The functional structures have a remarkable influence on their ice control properties. It is supposed that PMet-MOH inhibits ice growth possibly through the adsorption mechanism by adjacent methyl and hydroxyl groups, whereas trehalose-tethered PMet can restrict diffusion of water molecules with the strongest ice recrystallization inhibition activity and zwitterionic PMet-COOH promotes ice nucleation obviously. This work offers valuable insight into the development of functional polypeptides as promising biocompatible cryoprotectants.

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来源期刊

Biomacromolecules 化学-高分子科学

CiteScore

10.60

自引率

4.80%

发文量

417

审稿时长

1.6 months

期刊介绍： Biomacromolecules is a leading forum for the dissemination of cutting-edge research at the interface of polymer science and biology. Submissions to Biomacromolecules should contain strong elements of innovation in terms of macromolecular design, synthesis and characterization, or in the application of polymer materials to biology and medicine. Topics covered by Biomacromolecules include, but are not exclusively limited to: sustainable polymers, polymers based on natural and renewable resources, degradable polymers, polymer conjugates, polymeric drugs, polymers in biocatalysis, biomacromolecular assembly, biomimetic polymers, polymer-biomineral hybrids, biomimetic-polymer processing, polymer recycling, bioactive polymer surfaces, original polymer design for biomedical applications such as immunotherapy, drug delivery, gene delivery, antimicrobial applications, diagnostic imaging and biosensing, polymers in tissue engineering and regenerative medicine, polymeric scaffolds and hydrogels for cell culture and delivery.