Mehwish Shah , Zheng Linyan , Muhammad Qasim , Yemawaysh Zewdie , Liping Qiu , Muhammad Ayaz
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Furhter, we elucidated the inhibitory potentials of samples towards various oxidants, free radicals, microbial strains, and cancer cell lines to assess their potential use in nutraceuticals.</div></div><div><h3>Results</h3><div>Considerable amount of phenolic acids such as gallic acid (GA) and citric acid (CA) were found in the MeOH extract (0.5 and 16 µg/mg GA equivalent respectively). Moreover, flavonoids such as QU were abundantly found in the ethyl acetate (EA) extract (1.9 µg/mg QE equivalent). Highest TFC was found in EA and CL extracts (130.46 and 81.08 µg QE/mg respectively), while the highest TPC was observed in MeOH + WT extract (108.67 µg GA/mg). Extracts rich in phenolic acids revealed strong antioxidant capacity. In particular, the MeOH extract showed high activity (IC<sub>50</sub> = 5.48 µg/mL) in diphenylpicryl hydrazine (DPPH) free-radical scavenging assays. The MeOH + EtOH and MeOH + WT extracts exhibited considerable antibacterial activity against <em>Pseudomonas aeruginosa</em> and <em>Bacillus subtilis</em> (IC<sub>50</sub> = 11.1 and 11 µg/mL, respectively)<em>.</em> The extracts also demonstrated in vitro cytotoxicity against the HeLa cervical cancer cell line, with the NH extract demonstrating a high activity of IC<sub>50</sub> = 14.29 µg/mL. The CL extract demonstrated significant antifungal activity (DIZ = 11 ± 0.03) against <em>Mucor</em> species. The hemocompatibility results revealed that all extracts were safe. The structural-bioactivity relationship showed neuroprotective and anti-aging activity of the combination of different classes of phytochemicals in these extracts.</div></div><div><h3>Conclusions</h3><div>Our findings indicate that <em>S. quettense</em> plant extracts contain bioactive secondary metabolites which might contribute towards the pharmacological potentials of the plant. Further detailed studies are required to elucidate the molecular mechanism of these results and its potential applications in nutraceutical sector.</div></div>","PeriodicalId":371,"journal":{"name":"Journal of Molecular Liquids","volume":"426 ","pages":"Article 127349"},"PeriodicalIF":5.2000,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"HPLC/MALDI-TOF-MS characterization and nutraceutical potential of Seriphedium quettense extracts: A polarity directed solvent extraction and antioxidant kinetics\",\"authors\":\"Mehwish Shah , Zheng Linyan , Muhammad Qasim , Yemawaysh Zewdie , Liping Qiu , Muhammad Ayaz\",\"doi\":\"10.1016/j.molliq.2025.127349\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><div><em>Seriphedium quettense (S. quettense</em>) is traditionally used against various diseases and is rich in bioactive metabolities Although this plant has several known ethnomedicinal uses, its polarity-guided fingerprinting with kinetic modelling and biological evaluations are not yet been reported.</div></div><div><h3>Materials and Methods</h3><div>In this study, we performed polarity-directed extraction of fractions from <em>S. quettense</em> and samples were subjected to HPLC/MALDI-TOF MS analysis, total phenolic and flavonoids contents (TPC/TFC) following standard procedures. Furhter, we elucidated the inhibitory potentials of samples towards various oxidants, free radicals, microbial strains, and cancer cell lines to assess their potential use in nutraceuticals.</div></div><div><h3>Results</h3><div>Considerable amount of phenolic acids such as gallic acid (GA) and citric acid (CA) were found in the MeOH extract (0.5 and 16 µg/mg GA equivalent respectively). Moreover, flavonoids such as QU were abundantly found in the ethyl acetate (EA) extract (1.9 µg/mg QE equivalent). Highest TFC was found in EA and CL extracts (130.46 and 81.08 µg QE/mg respectively), while the highest TPC was observed in MeOH + WT extract (108.67 µg GA/mg). Extracts rich in phenolic acids revealed strong antioxidant capacity. In particular, the MeOH extract showed high activity (IC<sub>50</sub> = 5.48 µg/mL) in diphenylpicryl hydrazine (DPPH) free-radical scavenging assays. The MeOH + EtOH and MeOH + WT extracts exhibited considerable antibacterial activity against <em>Pseudomonas aeruginosa</em> and <em>Bacillus subtilis</em> (IC<sub>50</sub> = 11.1 and 11 µg/mL, respectively)<em>.</em> The extracts also demonstrated in vitro cytotoxicity against the HeLa cervical cancer cell line, with the NH extract demonstrating a high activity of IC<sub>50</sub> = 14.29 µg/mL. The CL extract demonstrated significant antifungal activity (DIZ = 11 ± 0.03) against <em>Mucor</em> species. The hemocompatibility results revealed that all extracts were safe. The structural-bioactivity relationship showed neuroprotective and anti-aging activity of the combination of different classes of phytochemicals in these extracts.</div></div><div><h3>Conclusions</h3><div>Our findings indicate that <em>S. quettense</em> plant extracts contain bioactive secondary metabolites which might contribute towards the pharmacological potentials of the plant. 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HPLC/MALDI-TOF-MS characterization and nutraceutical potential of Seriphedium quettense extracts: A polarity directed solvent extraction and antioxidant kinetics
Background
Seriphedium quettense (S. quettense) is traditionally used against various diseases and is rich in bioactive metabolities Although this plant has several known ethnomedicinal uses, its polarity-guided fingerprinting with kinetic modelling and biological evaluations are not yet been reported.
Materials and Methods
In this study, we performed polarity-directed extraction of fractions from S. quettense and samples were subjected to HPLC/MALDI-TOF MS analysis, total phenolic and flavonoids contents (TPC/TFC) following standard procedures. Furhter, we elucidated the inhibitory potentials of samples towards various oxidants, free radicals, microbial strains, and cancer cell lines to assess their potential use in nutraceuticals.
Results
Considerable amount of phenolic acids such as gallic acid (GA) and citric acid (CA) were found in the MeOH extract (0.5 and 16 µg/mg GA equivalent respectively). Moreover, flavonoids such as QU were abundantly found in the ethyl acetate (EA) extract (1.9 µg/mg QE equivalent). Highest TFC was found in EA and CL extracts (130.46 and 81.08 µg QE/mg respectively), while the highest TPC was observed in MeOH + WT extract (108.67 µg GA/mg). Extracts rich in phenolic acids revealed strong antioxidant capacity. In particular, the MeOH extract showed high activity (IC50 = 5.48 µg/mL) in diphenylpicryl hydrazine (DPPH) free-radical scavenging assays. The MeOH + EtOH and MeOH + WT extracts exhibited considerable antibacterial activity against Pseudomonas aeruginosa and Bacillus subtilis (IC50 = 11.1 and 11 µg/mL, respectively). The extracts also demonstrated in vitro cytotoxicity against the HeLa cervical cancer cell line, with the NH extract demonstrating a high activity of IC50 = 14.29 µg/mL. The CL extract demonstrated significant antifungal activity (DIZ = 11 ± 0.03) against Mucor species. The hemocompatibility results revealed that all extracts were safe. The structural-bioactivity relationship showed neuroprotective and anti-aging activity of the combination of different classes of phytochemicals in these extracts.
Conclusions
Our findings indicate that S. quettense plant extracts contain bioactive secondary metabolites which might contribute towards the pharmacological potentials of the plant. Further detailed studies are required to elucidate the molecular mechanism of these results and its potential applications in nutraceutical sector.
期刊介绍：
The journal includes papers in the following areas:
– Simple organic liquids and mixtures
– Ionic liquids
– Surfactant solutions (including micelles and vesicles) and liquid interfaces
– Colloidal solutions and nanoparticles
– Thermotropic and lyotropic liquid crystals
– Ferrofluids
– Water, aqueous solutions and other hydrogen-bonded liquids
– Lubricants, polymer solutions and melts
– Molten metals and salts
– Phase transitions and critical phenomena in liquids and confined fluids
– Self assembly in complex liquids.– Biomolecules in solution
The emphasis is on the molecular (or microscopic) understanding of particular liquids or liquid systems, especially concerning structure, dynamics and intermolecular forces. The experimental techniques used may include:
– Conventional spectroscopy (mid-IR and far-IR, Raman, NMR, etc.)
– Non-linear optics and time resolved spectroscopy (psec, fsec, asec, ISRS, etc.)
– Light scattering (Rayleigh, Brillouin, PCS, etc.)
– Dielectric relaxation
– X-ray and neutron scattering and diffraction.
Experimental studies, computer simulations (MD or MC) and analytical theory will be considered for publication; papers just reporting experimental results that do not contribute to the understanding of the fundamentals of molecular and ionic liquids will not be accepted. Only papers of a non-routine nature and advancing the field will be considered for publication.
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