Marisa Mercadante, Armin Scheben, Jacob Estrada, Jan Savas-Carstens, William Sullivan, Nicholas Housel, Tatiana Volpari, Jax Hebner, Maria Sapar, Tom Rusielewicz, Frederick J Monsma, Stefan Semrau, Yinan Wang, Laura A Martin
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A patient-derived ovarian cancer organoid platform to study susceptibility to natural killer cells.
Intratumoral heterogeneity drives therapy resistance and relapses in advanced stage cancers, such as ovarian cancer. Here, we present a live cell imaging assay using patient-derived ovarian cancer organoids for real time capture and quantification of natural killer cell-mediated apoptotic events in >500 organoids simultaneously. Our assay revealed significant inter- and intratumor response heterogeneity and identified a rare resistant organoid population, opening avenues to test immunomodulatory strategies that overcome resistance.
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