Saetbyeol Jeon, Hwira Baek, Seulgi Kim, Youngseok Kim, Junoh Kim, Jin Woong Kim
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Microalgae-Derived Microparticles Improve Immunomodulation via Combined Glycolysis and MAPK Activation
Natural polysaccharides possess potent immunostimulatory properties, but their poor solubility impedes efficiency of cellular delivery. This study focuses on extraction of microparticles (MPs) fromEuglena gracilis, a microalgae species characterized by abundant intracellular β-1,3-glucan and flexible cell membrane. We introduce anE. gracilis-derived MP (MPEG) system as a natural carrier for solubilized β-glucan. The MPEG system enhances β-glucan’s solubility and loading efficiency through sequential sonication and cell extrusion. In vitro studies reveal that MPEG utilizes multiple endocytosis pathways, including phagocytosis, clathrin-mediated, and lipid raft-mediated routes, for effective β-glucan delivery into cells. Upon cellular internalization, MPEG components trigger dual activation of the MAPK signaling pathway and glycolysis in macrophages, leading to enhanced production of pro-inflammatory cytokines and lactic acid, ultimately strengthening innate immune responses. This MPEG system offers a promising approach to harnessing the immunostimulatory properties of natural polysaccharides while overcoming their solubility limitations, opening new avenues for targeted cellular delivery in immunomodulation therapies.
期刊介绍：
Langmuir is an interdisciplinary journal publishing articles in the following subject categories:
Colloids: surfactants and self-assembly, dispersions, emulsions, foams
Interfaces: adsorption, reactions, films, forces
Biological Interfaces: biocolloids, biomolecular and biomimetic materials
Materials: nano- and mesostructured materials, polymers, gels, liquid crystals
Electrochemistry: interfacial charge transfer, charge transport, electrocatalysis, electrokinetic phenomena, bioelectrochemistry
Devices and Applications: sensors, fluidics, patterning, catalysis, photonic crystals
However, when high-impact, original work is submitted that does not fit within the above categories, decisions to accept or decline such papers will be based on one criteria: What Would Irving Do?
Langmuir ranks #2 in citations out of 136 journals in the category of Physical Chemistry with 113,157 total citations. The journal received an Impact Factor of 4.384*.
This journal is also indexed in the categories of Materials Science (ranked #1) and Multidisciplinary Chemistry (ranked #5).
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