Microbial cell factories for bioconversion of lignin to vanillin — Challenges and opportunities: A review

The bioconversion of lignin into vanillin via microbial cell factories offers a promising and sustainable route for producing high-value aromatic compounds from the abundant and underutilized byproducts of plant biomass. This review comprehensively explores the synthesis, structural characteristics, and diverse industrial applications of lignin, while addressing the inherent challenges posed by its complex structure in bioconversion processes. It examines the potential of microbial cell factories for lignin degradation, emphasizing the latest advancements in genetic engineering and metabolic optimization strategies that enhance microbial efficiency in lignin degradation and vanillin biosynthesis. It further assesses the economic feasibility of lignin-to-vanillin conversion by discussing key factors influencing cost-effectiveness and scalability, highlighting the transformative potential for producing high-value aromatic compounds in an environmentally sustainable manner. The review also highlights ongoing research efforts to develop robust microbial strains and optimize metabolic pathways for improved vanillin yield. By integrating multidisciplinary approaches, this review highlights the transformative potential of microbial cell factories to valorize lignin, offering a sustainable pathway for the production of vanillin and related aromatic compounds.