Jing-Yi Shi , Chao-Yue Wang , Yifan Xia , Meng-Ya Guo , Wen Yang , Yu-Shun Yang , Hai-Liang Zhu
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引用次数: 0
摘要
本研究开发了一种具有咪唑吡啶-异香兰素子结构的荧光探针ImPI-CE，并将其用于监测结直肠细胞中的羧酸酯酶（CE）水平，以评估中药水煎剂的疗效。ImPI-CE 的优点是反应快速、稳定性高、灵敏度高、选择性高、抗干扰性强。其中最重要的挑战点是基于生物药效的测试和结直肠场景成像，这两点都适合中药水煎剂的国际认可。其中，Impi-CE 实现了对多个中药水煎剂样品的稳定监测。它能对活体大肠细胞在抑制和激活状态下的 CE 水平进行成像。ImPI-CE还实现了对单独中药水煎剂样品和复方中药水煎剂样品进行基于细胞内生物功效的研究。这些研究结果有利于中药的国际认可。
A fluorescent probe for detecting carboxylesterase level on investigating Chinese medicine water decoction efficacy
In this work, a fluorescent probe ImPI-CE with the imidazo-pyridin-isovanillin sub-structure was developed and studied for monitoring the Carboxylesterase (CE) level in colorectal cells on evaluating the Chinese medicine water decoction efficacy. The advantages of ImPI-CE included rapid response, high stability, high sensitivity, high selectivity, and reliable anti-interference. The most significant challenging point here was the biological efficacy-based test and the imaging in colorectal scenario which were both suitable for the international recognition of Chinese medicine water decoction. In particular, ImPI-CE achieved the stable monitoring of multiple Chinese medicine water decoction samples. It was able to imaging the CE level in both the inhibition and activation situations in living colorectal cells. ImPI-CE also realized the intracellular biological efficacy-based investigation of both the separate and combination Chinese medicine water decoction samples. The findings were beneficial for the international recognition of Chinese medicine.
期刊介绍：
Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science.
The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments.
Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate.
Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to:
Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences,
Novel experimental techniques or instrumentation for molecular spectroscopy,
Novel theoretical and computational methods,
Novel applications in photochemistry and photobiology,
Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.
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