首次鉴定了宿主肠杆菌IncFII质粒携带blaNDM-1的4个重复区

IF 4.1 2区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES iScience Pub Date : 2025-04-08 DOI:10.1016/j.isci.2025.112369

Yi Liu , Xiaojing Liu , Ruishan Liu , Hao Xu , Mantao Chen , Jiajie Qian , Beiwen Zheng

{"title":"首次鉴定了宿主肠杆菌IncFII质粒携带blaNDM-1的4个重复区","authors":"Yi Liu , Xiaojing Liu , Ruishan Liu , Hao Xu , Mantao Chen , Jiajie Qian , Beiwen Zheng","doi":"10.1016/j.isci.2025.112369","DOIUrl":null,"url":null,"abstract":"<div><div>Carbapenem-resistant Enterobacteriaceae (CRE) have emerged as a critical threat to global health, and multicopy resistance genes are a potential contributor to increased resistance. This study investigates a novel arrangement of four blaNDM-1 genes in tandem on an IncFII plasmid from Enterobacter hormaechei. Whole-genome sequencing (WGS) of E. hormaechei isolates L710hy identified a four-tandem repeat of the blaNDM-1 gene, with each copy’s environment nearly identical (ISCR1-aph(3′)-VI-ISAba125-blaNDM-1-ble-iso-tat-dsbD-cutA-sul1-ISCR1). The study suggests a correlation between the blaNDM-1 copy number and minimum inhibitory concentration (MIC) values for carbapenems, though further research is needed to confirm this correlation. The stability of these four blaNDM-1 tandem repeat structures was also investigated. This is the first documented case of blaNDM-1 tandem repeats in E. hormaechei, and the ISCR1 and other insertion sequence may play a role in this arrangement.</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"28 5","pages":"Article 112369"},"PeriodicalIF":4.1000,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"First characterization of four repeat regions with the blaNDM-1 carried on an IncFII plasmid in Enterobacter hormaechei\",\"authors\":\"Yi Liu , Xiaojing Liu , Ruishan Liu , Hao Xu , Mantao Chen , Jiajie Qian , Beiwen Zheng\",\"doi\":\"10.1016/j.isci.2025.112369\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Carbapenem-resistant Enterobacteriaceae (CRE) have emerged as a critical threat to global health, and multicopy resistance genes are a potential contributor to increased resistance. This study investigates a novel arrangement of four blaNDM-1 genes in tandem on an IncFII plasmid from Enterobacter hormaechei. Whole-genome sequencing (WGS) of E. hormaechei isolates L710hy identified a four-tandem repeat of the blaNDM-1 gene, with each copy’s environment nearly identical (ISCR1-aph(3′)-VI-ISAba125-blaNDM-1-ble-iso-tat-dsbD-cutA-sul1-ISCR1). The study suggests a correlation between the blaNDM-1 copy number and minimum inhibitory concentration (MIC) values for carbapenems, though further research is needed to confirm this correlation. The stability of these four blaNDM-1 tandem repeat structures was also investigated. This is the first documented case of blaNDM-1 tandem repeats in E. hormaechei, and the ISCR1 and other insertion sequence may play a role in this arrangement.</div></div>\",\"PeriodicalId\":342,\"journal\":{\"name\":\"iScience\",\"volume\":\"28 5\",\"pages\":\"Article 112369\"},\"PeriodicalIF\":4.1000,\"publicationDate\":\"2025-04-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"iScience\",\"FirstCategoryId\":\"103\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2589004225006303\",\"RegionNum\":2,\"RegionCategory\":\"综合性期刊\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"MULTIDISCIPLINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"iScience","FirstCategoryId":"103","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2589004225006303","RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MULTIDISCIPLINARY SCIENCES","Score":null,"Total":0}

引用次数: 0

摘要

碳青霉烯耐药肠杆菌科（CRE）已成为对全球健康的严重威胁，多拷贝耐药基因是增加耐药性的潜在因素。本研究研究了四种blaNDM-1基因在激素肠杆菌IncFII质粒上串联的新排列。全基因组测序（WGS）鉴定出了一个blaNDM-1基因的四串联重复序列，每个拷贝的环境几乎相同（ISCR1-aph(3 ')- vi - isaba125 -blaNDM-1-ble-iso- stat - dsbd - cuta -sul1- iscr1）。该研究提示blaNDM-1拷贝数与碳青霉烯类的最低抑制浓度（MIC）值之间存在相关性，但需要进一步的研究来证实这种相关性。并对这4个blaNDM-1串联重复结构的稳定性进行了研究。这是首次在荷氏肠杆菌中发现blaNDM-1串联重复序列，ISCR1和其他插入序列可能在这种排列中起作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

摘要图片

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

First characterization of four repeat regions with the blaNDM-1 carried on an IncFII plasmid in Enterobacter hormaechei

Carbapenem-resistant Enterobacteriaceae (CRE) have emerged as a critical threat to global health, and multicopy resistance genes are a potential contributor to increased resistance. This study investigates a novel arrangement of four bla_NDM-1 genes in tandem on an IncFII plasmid from Enterobacter hormaechei. Whole-genome sequencing (WGS) of E. hormaechei isolates L710hy identified a four-tandem repeat of the bla_NDM-1 gene, with each copy’s environment nearly identical (ISCR1-aph(3′)-VI-ISAba125-bla_NDM-1-ble-iso-tat-dsbD-cutA-sul1-ISCR1). The study suggests a correlation between the bla_NDM-1 copy number and minimum inhibitory concentration (MIC) values for carbapenems, though further research is needed to confirm this correlation. The stability of these four bla_NDM-1 tandem repeat structures was also investigated. This is the first documented case of bla_NDM-1 tandem repeats in E. hormaechei, and the ISCR1 and other insertion sequence may play a role in this arrangement.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

iScience Multidisciplinary-Multidisciplinary

CiteScore

7.20

自引率

1.70%

发文量

1972

审稿时长

6 weeks

期刊介绍： Science has many big remaining questions. To address them, we will need to work collaboratively and across disciplines. The goal of iScience is to help fuel that type of interdisciplinary thinking. iScience is a new open-access journal from Cell Press that provides a platform for original research in the life, physical, and earth sciences. The primary criterion for publication in iScience is a significant contribution to a relevant field combined with robust results and underlying methodology. The advances appearing in iScience include both fundamental and applied investigations across this interdisciplinary range of topic areas. To support transparency in scientific investigation, we are happy to consider replication studies and papers that describe negative results. We know you want your work to be published quickly and to be widely visible within your community and beyond. With the strong international reputation of Cell Press behind it, publication in iScience will help your work garner the attention and recognition it merits. Like all Cell Press journals, iScience prioritizes rapid publication. Our editorial team pays special attention to high-quality author service and to efficient, clear-cut decisions based on the information available within the manuscript. iScience taps into the expertise across Cell Press journals and selected partners to inform our editorial decisions and help publish your science in a timely and seamless way.