Simultaneous Determination of Biologically Active Components of Rhodiola rosea in Extracts of Plant Raw Materials by HILIC–MS/MS

A simple, rapid, and highly sensitive method for the simultaneous determination of the main components of Rhodiola rosea (rosin, salidroside, rosarin, and rosavin) in a wide concentration range by hydrophilic chromatography–tandem mass spectrometry is developed. The conditions for the extraction (extractant type and composition, extraction time) of the main components from Rhodiola rosea root samples, chromatographic separation and detection of these compounds are selected, and the performance characteristics of the proposed approach are evaluated. The limits of detection are 250, 2.4, 2.3, and 5.4 ng/mL, and the linearity ranges are 1–100, 0.01–10, 0.01–10, and 0.01–100 μg/mL for rosin, salidroside, rosarin and rosavin, respectively. The developed approach is tested in an analysis of real samples of the pharmaceutical product and of plant raw material.