{"title":"抗独特型处理小鼠抗原特异性免疫诱导的遗传学及单克隆Ab3抗体的衍生","authors":"S.L. Epstein , S.O. Sharrow , D.H. Sachs","doi":"10.1016/0769-2625(88)90057-8","DOIUrl":null,"url":null,"abstract":"<div><p>Antiidiotypic antibodies can be used as a means other than antigen of clone-specific intervention in the immune system. In a number of cases, antiidiotypic antibodies induce potent, antigen-specific responses without exposure of the recipient animals to antigen, and a better understanding of such responses will aid in the application of idiotype manipulation to both immunological and practical problems. The Ia.7 system expresses a major, cross-reactive idiotype on antibodies to an epitope of the mouse I-E antigen. Here, we examine genetic control of the I-E-specific response to anti-idiotype in this model system. Results demonstrate that both heavy chain allotype-linked and non-allotype-linked loci contribute to the control of this response. In addition, monoclonal antibodies representing the non-antigen-specific portion of the Ab3 response were derived. Two idiotype-positive clones were demonstrated to be of IgM isotype, contrasting with previous data showing that the serum Ab3 response is predominantly IgG1.</p></div>","PeriodicalId":77665,"journal":{"name":"Annales de l'Institut Pasteur. Immunology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1988-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0769-2625(88)90057-8","citationCount":"2","resultStr":"{\"title\":\"Genetics of the induction of antigen-specific immunity in anti-idiotype-treated mice and derivation of monoclonal Ab3 antibodies\",\"authors\":\"S.L. Epstein , S.O. Sharrow , D.H. Sachs\",\"doi\":\"10.1016/0769-2625(88)90057-8\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Antiidiotypic antibodies can be used as a means other than antigen of clone-specific intervention in the immune system. In a number of cases, antiidiotypic antibodies induce potent, antigen-specific responses without exposure of the recipient animals to antigen, and a better understanding of such responses will aid in the application of idiotype manipulation to both immunological and practical problems. The Ia.7 system expresses a major, cross-reactive idiotype on antibodies to an epitope of the mouse I-E antigen. Here, we examine genetic control of the I-E-specific response to anti-idiotype in this model system. Results demonstrate that both heavy chain allotype-linked and non-allotype-linked loci contribute to the control of this response. In addition, monoclonal antibodies representing the non-antigen-specific portion of the Ab3 response were derived. Two idiotype-positive clones were demonstrated to be of IgM isotype, contrasting with previous data showing that the serum Ab3 response is predominantly IgG1.</p></div>\",\"PeriodicalId\":77665,\"journal\":{\"name\":\"Annales de l'Institut Pasteur. Immunology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1988-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0769-2625(88)90057-8\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Annales de l'Institut Pasteur. Immunology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0769262588900578\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Annales de l'Institut Pasteur. Immunology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0769262588900578","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Genetics of the induction of antigen-specific immunity in anti-idiotype-treated mice and derivation of monoclonal Ab3 antibodies
Antiidiotypic antibodies can be used as a means other than antigen of clone-specific intervention in the immune system. In a number of cases, antiidiotypic antibodies induce potent, antigen-specific responses without exposure of the recipient animals to antigen, and a better understanding of such responses will aid in the application of idiotype manipulation to both immunological and practical problems. The Ia.7 system expresses a major, cross-reactive idiotype on antibodies to an epitope of the mouse I-E antigen. Here, we examine genetic control of the I-E-specific response to anti-idiotype in this model system. Results demonstrate that both heavy chain allotype-linked and non-allotype-linked loci contribute to the control of this response. In addition, monoclonal antibodies representing the non-antigen-specific portion of the Ab3 response were derived. Two idiotype-positive clones were demonstrated to be of IgM isotype, contrasting with previous data showing that the serum Ab3 response is predominantly IgG1.